Recombinant Mouse V (D)J recombination-activating protein 1, partial

1. this study determined that the depletion of uterine innate lymphoid cells in Rag1-/- mice resulted in impaired uterine spiral artery remodeling and reduced fetal viability PMID: 28915450
2. Describe spontaneous S. xylosus infection in a genetically modified murine model. S. xylosus infection in Rag1-/-Tpl2-/- mice correlated with disseminated bacteria and elevated numbers of circulating monocytes. PMID: 28830581
3. This study demonstrated that the lymphocyte-deficient Rag1 animals demonstrated a significantly lower rate of aneurysm formation and rupture. PMID: 27416931
4. a novel RAG1/2-mediated insertion pathway distinct from DNA transposition and trans-V(D)J recombination that destabilizes the genome and shares features with reported oncogenic DNA insertions. PMID: 28179379
5. loss of the BH3-only protein BIM accelerated lymphoma development in p53-deficient mice. This process was negated by concomitant loss of RAG1/2-mediated antigen receptor gene rearrangement. PMID: 27621418
6. Nonamer binding domain (NBD) of RAG1 plays a central role in the recognition of recombination signal sequence. PMID: 26742581
7. Overall, these findings suggest that the V(D)J recombination-activating gene 1, RAG1, may play a role in long-term memory consolidation. PMID: 26843989
8. propose that pre-B cells toggle between pre-BCR signals and a RAG1 DNA double-strand break-dependent checkpoint to maintain genome stability while iteratively assembling Igl chain genes PMID: 26834154
9. Study reports that RAG1 associates with chromatin at thousands of active promoters and enhancers in the genome of developing lymphocytes. The mouse and human genomes appear to have responded by reducing the abundance of "cryptic" recombination signals near RAG1 binding sites. PMID: 26234156
10. The non-core region of RAG1 facilitates chromosomal V(D)J recombination in a ubiquitylation-dependent pathway. PMID: 25572281
11. Based on our results, we propose that interaction of RAG2 with RAG1 induces cooperative interactions of multiple binding sites, induced through conformational changes at the RAG1 interdomain boundary PMID: 25676158
12. 12recombination signal sequences-23recombination signal sequences cooperation (the "12/23 rule") is important not only for regulating RAG-mediated DNA cleavage but also for the efficiency of RAG1 and RAG2 recruitment to chromatin. PMID: 26303526
13. ubiquitination of RAG1, which can be promoted by RAG1's own ubiquitin ligase activity, plays a significant role in governing the level of V(D)J recombination activity PMID: 26124138
14. The complex RAG1/2 binds one copy each of 12 recombination signal sequence and 23 recombination signal sequence DNA. PMID: 25903130
15. SATB1 binds to the ASE and Rag promoters, facilitating inclusion of Rag2 in the chromatin hub and the loading of RNA polymerase II to both the Rag1 and Rag2 promoters. PMID: 25847946
16. Study provides evidence that RAG expression (RAG1, RAG2)in uncommitted hematopoietic progenitors and NK cell precursors marks functionally distinct subsets of NK cells in the periphery, demonstrating both a novel role for RAG in the functional specialization of the NK cell lineage and a physiological role for RAG proteins outside of V(D)J recombination. PMID: 25259923
17. Rag1 plays a role in optic neuropathy as a proapoptotic candidate in p50-deficient mice. PMID: 25312244
18. crystal structure of the mouse RAG1-RAG2 complex at 3.2 A resolution PMID: 25707801
19. Integrated genetic approaches identify the molecular mechanisms of Sox4 in early B-cell development: intricate roles for RAG1/2 and CK1epsilon. PMID: 24786772
20. Fas signals act as a silencing mechanism for CD40-induced RAGs and prevent CD40 translocation to the nucleus. PMID: 24037181
21. noncore RAG1 regions establish a diverse TCR repertoire by overcoming Vbeta RSS inefficiency to promote Vbeta recombination and alphabeta T cell development, and by modulating TCRbeta and TCRalpha gene segment utilization. PMID: 24415779
22. Rag1-deficient NOD thymocytes have T cell defects that can collapse regulatory boundaries at two early T cell checkpoints, which may predispose them to both leukemia and autoimmunity. PMID: 23440410
23. HMGB1 binds more tightly to a RAG1-DNA complex over RAG1 or DNA alone. PMID: 23325855
24. histological analysis of brain sections did not reveal a difference in reactive astrogliosis, tissue destruction, and neuronal cell death in Rag1(-/-) compared to wild-type mice PMID: 22335783
25. The defects of scid HSCs are independent of their inability to perform lymphopoiesis because a similar defect in hematopoietic niche occupancy was not observed with Rag1(-/-) recipients. PMID: 22147896
26. multi-subunit cullin RING E3 ligase complex VprBP/DDB1/Cul4A/Roc1 associates with full-length RAG1 through VprBP PMID: 22157821
27. In the present study, the authors report that heteroduplex DNA containing a bubble region can be cleaved efficiently when present along with a recombination signal sequence (RSS) in cis or trans configuration. PMID: 22119487
28. Kinetic analysis using the first highly active full-length purified Rag1/Rag2 complexes has now allowed us to define the important catalytic features of this complex. PMID: 22064481
29. central non-core domain of RAG1 functions in nuclear localization, zinc coordination, and interactions with nucleic acid PMID: 21599978
30. combined loss of p19Arf and Rag1 results in B-cell precursor leukemia in mice PMID: 21622646
31. phosphorylation of RAG1 or RAG2 by ATM or DNA-PKcs at SQ/TQ consensus sites is dispensable for the joining step of V(D)J recombination. PMID: 21742970
32. data trace the origin of the impairment in social recognition memory in RAG1-deficient mice to the RAG1 gene locus and implicate RAG1 in memory formation PMID: 21354115
33. sequences of the endogenous TCR transcripts suggested that gene recombination could occur, albeit quite inefficiently, in the RAG-deficient mice PMID: 20454452
34. Data show that negative regulatory function of the noncore regions of RAG1/2 limits the RAG endonuclease activity in the absence of an activating methylated histone tail bound to the complex. PMID: 21149691
35. Data show that promoters and transcription direct RAG1 binding locally, and that RAG1 binding can be targeted in the absence of RAG2. PMID: 21115692
36. Abcc6 and Rag1 have roles in ectopic mineralization in a murine model of pseudoxanthoma elasticum PMID: 20185580
37. Siglec-G-deficient B1a lymphocytes survive longer in vitro and become the dominant population when injected in competition with wild-type B1a cells into Rag1-deficient mice. PMID: 20729333
38. The stochastic generation of an autoreactive B cell repertoire is an important aspect of immunodeficiencies associated with hypomorphic RAG mutations. PMID: 20547827
39. Liver natural killer cells may represent an independent pool of cells from those developing out of the bone marrow, and RAG-1 itself may have a significant role in NK cell development. PMID: 19949422
40. In V(D)J recombination, while RAG1 binding was detected only at regions containing recombination signal sequences, RAG2 binds at thousands of sites in the genome containing histone 3 trimethylated at lysine 4. PMID: 20398922
41. Essential events in B lymphopoiesis are not tightly synchronized; some progenitors with increased probability of becoming lymphocytes express RAG-1 while still part of the lineage marker-negative Sca-1+c-Kit(high) fraction. PMID: 20007571
42. These results further underscore the similarities between RAG1/2 and integrase and suggest that certain integrase inhibitors may have the potential to interfere with aspects of B and T cell development PMID: 11756686
43. increased accumulation of hybrid V(D)J joins in cells expressing truncated versus full-length RAGs PMID: 11779512
44. The peripheral t-cells of Rag1KO/sf/Y OVA mice do not tolerize normally, even when twice the dose that causes tolerance in control mice is administered. PMID: 11900414
45. RAG-mediated V(D)J recombination is not essential for tumorigenesis in Atm-deficient mice. PMID: 11940674
46. Mutational analysis of RAG-1 revealed several amino acids outside of the canonical nonamer-binding domain that are critical for DNA binding, step arrest mutants with defects in nicking or hairpin formation, and four RAG-1 mutants defective for joining. PMID: 11971977
47. Signal ends are more abundant than coding ends. Signal joints form only after RAG expression is downregulated. Signal joints are not inert; they are cleaved in vivo and in vitro by a nick-nick mechanism to form a substrate for RAG-mediated transposition. PMID: 11983177
48. bcl-xL and RAG genes are induced and the response to IL-2 enhanced in EmuEBNA-1 transgenic mouse lymphocytes PMID: 12140768
49. A RAG1/2 multimer that can cleave both RSS assembles on an isolated RSS. Complementary RSS enters this complex as naked DNA. When RAG1/2 binds 12 & 23 RSSs separately prior to their mixing, synaptic complex assembly & cleavage activity are reduced. PMID: 12145216
50. RAG1 mutants proficient in DNA cleavage but defective in interaction with coding ends after cleavage & in capturing target DNA for transposition have defects in hybrid joint formation, hairpin coding end opening, transposition, & in V(D)J recombination. PMID: 12154124

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KEGG: mmu:19373

STRING: 10090.ENSMUSP00000077584

UniGene: Mm.828