Recombinant Mouse Vesicle transport through interaction with t-SNAREs homolog 1A (Vti1a), partial

Code CSB-YP025942MO1
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Source Yeast
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Code CSB-EP025942MO1
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Source E.coli
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Code CSB-EP025942MO1-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP025942MO1
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Source Baculovirus
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Code CSB-MP025942MO1
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Vti1a
Uniprot No.
Alternative Names
Vti1a; Vti1; Vti1l2; Vesicle transport through interaction with t-SNAREs homolog 1A; Vesicle transport v-SNARE protein Vti1-like 2; Vti1-rp2
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
V-SNARE that mediates vesicle transport pathways through interactions with t-SNAREs on the target membrane. These interactions are proposed to mediate aspects of the specificity of vesicle trafficking and to promote fusion of the lipid bilayers. Involved in vesicular transport from the late endosomes to the trans-Golgi network. Along with VAMP7, involved in an non-conventional RAB1-dependent traffic route to the cell surface used by KCNIP1 and KCND2. May be concerned with increased secretion of cytokines associated with cellular senescence.
Gene References into Functions
  1. In adrenal chromaffin cells, vti1a localizes to a compartment near the trans-Golgi-network, but is absent from secretory granules.Vti1a is not required for final fusion of dense-core vesicles, but is needed for their generation. PMID: 24902738
  2. VTI1A, a vesicular transport associated factor is a novel regulator of hepatitis C virus release but not replication. PMID: 22646850
  3. Lack of the endosomal SNAREs vti1a and vti1b led to significant impairments in neuronal development PMID: 21262811
Subcellular Location
Golgi apparatus membrane; Single-pass type IV membrane protein.
Protein Families
VTI1 family
Tissue Specificity
Widely expressed.
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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