Recombinant Mouse WW domain-binding protein 11 (Wbp11)

Code CSB-YP852865MO
MSDS
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Source Yeast
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Code CSB-EP852865MO
MSDS
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Source E.coli
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Code CSB-EP852865MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP852865MO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP852865MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Wbp11
Uniprot No.
Alternative Names
Wbp11; Sipp1; WW domain-binding protein 11; WBP-11; Splicing factor that interacts with PQBP-1 and PP1
Species
Mus musculus (Mouse)
Expression Region
1-641
Target Protein Sequence
MGRRSTSSTK SGKFMNPTDQ ARKEARKREL KKNKKQRMMV RAAVLKMKDP KQIIRDMEKL DEMEFNPVQQ PQLNEKVLKD KRKKLRETFE RILRLYEKEN PDIYKELRKL EVEYEQKRAQ LSQYFDAVKN AQHVEVESIP LPDMPHAPSN ILIQDIPLPG AQPPSILKKT SAYGPPARAV SILPLLGHGV PRLPPGRKPP GPPPGPPPPQ VLQMYGRKVG FALDLPPRRR DEDMLYSPEL AQRGHDDDMS STSEDDGYPE DMDQDKHDDS TEDSDTDRSD AESDGDEFGH REDSERDNTE EKKSGLSVRF ADMPGKSRKK KKNMKELTPL QAMMLRMAGQ EIPEEGREVE EFSEEEDADD SDDSEAEKQS QKQHKDDGHS DSTAAASSQQ QAPPQSAPAS QIQAPPMPGP PPLGPPPAPP LRPPGPPTGL PPGPPPGAPP FLRPPGMPGI RGPLPRLLPP GPPPGRPPGP PPGPPPGLPP GPPPRGPPPR LPPPAPPGIP PPRPGMMRPP LVPPLGPAPP GLFPPAPLPN PGVLSAPPSL IQRPKADDAS AATIEKKATA TISAKPQITN PKAEVTRFVP TALRVRRENK GATAVPQRRS EDDSAVPVAK AAPRSGPSVA VSVQTKDDVY EAFMKEMEGL L
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Activates pre-mRNA splicing. May inhibit PP1 phosphatase activity.
Gene References into Functions
  1. Identification of SIPP1 as a pre-mRNA splicing factor and a binding partner of protein phosphatase-1. PMID: 14640981
  2. SIPP1 is a positive regulator of pre-mRNA splicing that is regulated by nucleocytoplasmic shuttling PMID: 16162498
Subcellular Location
Nucleus. Cytoplasm. Note=Predominantly located in the nucleus with granular heterogeneous distribution. Excluded from nucleoli in interphase cells, distributed throughout cytoplasm in dividing cells. Colocalized with SC35 and U2B in the nucleus. In the cytoplasm, associates with the intermediate filament protein vimentin.
Tissue Specificity
Ubiquitously expressed, with highest levels in testis.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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