Product Details

Purity

>85% (SDS-PAGE)

Target Names

Wwox

Uniprot No.

Q91WL8

Alternative Names

Wwox; Wox1; WW domain-containing oxidoreductase; EC 1.1.1.-

Species

Mus musculus (Mouse)

Expression Region

1-414

Target Protein Sequence

MAALRYAGLD DTDSEDELPP GWEERTTKDG WVYYANHTEE KTQWEHPKTG KRKRVAGDLP YGWEQETDEN GQVFFVDHIN KRTTYLDPRL AFTVDDNPTK PTTRQRYDGS TTAMEILQGR DFTGKVVLVT GANSGIGFET AKSFALHGAH VILACRNLSR ASEAVSRILE EWHKAKVEAM TLDLAVLRSV QHFAEAFKAK NVSLHVLVCN AGTFALPWGL TKDGLETTFQ VNHLGHFYLV QLLQDVLCRS SPARVIVVSS ESHRFTDIND SSGKLDLSRL SPPRSDYWAM LAYNRSKLCN ILFSNELHRR LSPRGVTSNA VHPGNMMYSA IHRNSWVYKL LFTLARPFTK SMQQGAATTV YCAVAPELEG LGGMYFNNCC RCLPSEEAQS EETARALWEL SERLIQDRLG SPSS

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Required for normal bone development.

Gene References into Functions

Knockdown of lung WWOX expression in mice was observed to cause neutrophil influx and was accompanied by a corresponding vascular leak and inflammatory cytokine production. In cultured human alveolar epithelial cells, loss of WWOX expression resulted in increased c-Jun- and IL-8-dependent neutrophil chemotaxis toward cell monolayers. PMID: 28283473
Brca1-Wwox interaction supports non-homologous end-joining as the dominant DSB repair pathway in Wwox-sufficient cells PMID: 27869163
Both Wwox null and Wwoxhep-/- mice demonstrated reductions in ApoA-I and Abca1 levels, critical components in reverse cholesterol transport and generation of nascent HDL particles. PMID: 24871327
Aberrant WWOX expression contributes to the altered metabolism in cancer. PMID: 25595186
WWOX plays a critical role in normal central nervous system development and disease. PMID: 25416187
WWOX has many functions, and its deletion affects cellular metabolism and neoplastic progression. PMID: 25491415
Wwox-deficient mice develop normally but succumb to a lethal hypoglycemia early in life. WWOX as a tumor suppressor with emerging role in regulation of aerobic glycolysis. PMID: 25012504
These findings identify a novel role for the tumor suppressor WWOX and show that loss of WWOX expression may drive genomic instability PMID: 25331887
Modeling WWOX inactivation revealed a complex phenotype including postnatal lethality, defects in bone metabolism and steroidogenesis and tumor suppressor function resulting in osteosarcomas. PMID: 23254685
WWOX ablation leads to impaired mammary ductal growth. Moreover, targeted deletion of WWOX is associated with increased levels of fibronectin, a component of the extracellular matrix. PMID: 23254778
the WWOX tumor suppressor gene has in preventing tumorigenesis in breast cancer PMID: 21499303
analysis of the effect of the Wwox tumor suppressor gene in a knockout model PMID: 19936220
analysis of WWOX genomics, partners, and functions [review] PMID: 19708029
The intron/exon boundaries of Wox1 have been identified; the genomic organization has been found to be almost identical to that of human WWOX. PMID: 11979549
phosphorylation of JNK1 and WOX1 is necessary for their physical interaction and functional antagonism PMID: 12514174
WW domain-containing oxidoreductase downregulation induces Tau phosphorylation in vitro PMID: 15126504
sex steroid hormone-induced activation of WOX1 and WOX2 is independent of estrogen receptor and androgen receptor PMID: 15580310
Activated WOX1 is likely to exert apoptosis of neuronal cells in the outer retina during the light-induced injury and in mice with an inherited retinal defect. PMID: 15664696
UV-induced alterations of the FHIT and WWOX fragile site gene expression are involved at least partially in the checkpoint function of DNA damage PMID: 15798093
WOX1 plays a critical role in conferring cellular sensitivity to apoptotic stress and Tyr33 phosphorylation in WOX1 is essential for binding and stabilizing Ser46-phosphorylated p53 PMID: 16219768
analysis in mice shows that WWOX has a tumor suppressor function PMID: 17360458
In response to TNF, Zfra is upregulated and modulates TNF-mediated cell death via interacting with TRADD, FADD and RIP (death-inducing signaling complex) at the receptor level, and downstream effectors NF-kappaB, p53, WOX1, and JNK1. PMID: 17567906
Wwox hypomorphs had an increased incidence of B-cell lymphomas supports a role of Wwox as a tumor suppressor PMID: 17823927
WWOX tumor suppressor is essential for postnatal survival and normal bone metabolism PMID: 18487609
Essential requirement for the Wwox tumor suppressor in proper steroidogenesis. PMID: 18974271
WOX1 possesses a tetrad NSYK motif in the C-terminal short-chain alcohol dehydrogenase/reductase (SDR) domain, which may bind estrogen and androgen. Apoptotic and stress stimuli activates WOX1 via Tyr33 phosphorylation and nuclear translocation. PMID: 15580310

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Subcellular Location

Cytoplasm. Nucleus. Mitochondrion.

Protein Families

Short-chain dehydrogenases/reductases (SDR) family

Tissue Specificity

Ubiquitous. In the brain, expressed in cortex, striatum, hippocampus and cerebellum (at protein level). Detected in embryonic skeleton, in cranofacial bones, vertebrae and limb bones. Detected in chondrocytes and osteoblasts.

Database Links

KEGG: mmu:80707

STRING: 10090.ENSMUSP00000004756

UniGene: Mm.440420

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Recombinant Mouse WW domain-containing oxidoreductase (Wwox)

Product Details

Customer Reviews and Q&A

Target Background

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