Recombinant Mouse Wiskott-Aldrich syndrome protein family member 2 (Wasf2)

Code CSB-YP806368MO
MSDS
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Source Yeast
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Code CSB-EP806368MO
MSDS
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Source E.coli
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Code CSB-EP806368MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP806368MO
MSDS
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Source Baculovirus
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Code CSB-MP806368MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Wasf2
Uniprot No.
Alternative Names
Wasf2; Wave2; Wiskott-Aldrich syndrome protein family member 2; WASP family protein member 2; Protein WAVE-2
Species
Mus musculus (Mouse)
Expression Region
1-497
Target Protein Sequence
MPLVTRNIEP RHLCRQTLPS DTSELECRTN ITLANVIRQL GSLSKYAEDI FGEICTQASA FASRVNSLAE RVDRVQVKVT QLDPKEEEVS LQGINTRKAF RSSTTQDQKL FDRNSLPVPV LETYNSCDAP PPLNNLSPYR DDGKEALKFY TNPSYFFDLW KEKMLQDTKD IMKEKRKHRK EKKDNPNRGN VNPRKIKTRK EEWEKMKMGQ EFVESKERLG PSGYSSTLVY QNGSIGSVEN VDAASYPPPP QSDSASSPSP SFSEDNLPPP PAEFSYPADN QRGSVLAGPK RTSMVSPSHP PPAPPLSSPP GPKPGFAPPP APPPPPPMSV PPPLPSMGFG SPGTPPPPSP PSFPPHPDFA APPPPPPPPA ADYPMPPPPL SQPSGGAPPP PPPPPPPGPP PLPFSGADGQ PAAPPPPPPS EATKPKSSLP AVSDARSDLL SAIRQGFQLR RVEEQREQEK RDVVGNDVAT ILSRRIAVEY SDSEDDSSEF DEDDWSD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Downstream effector molecule involved in the transmission of signals from tyrosine kinase receptors and small GTPases to the actin cytoskeleton. Promotes formation of actin filaments. Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex.
Gene References into Functions
  1. WAVE2/c-Abl survival signal required at the hematopoietic stem cell (HSC) fetal liver to marrow transition that confers fitness to the HSC for marrow hematopoiesis PMID: 29915352
  2. TrkC signals to the podocyte actin cytoskeleton to induce migration by phosphorylating WAVE2 Erk dependently. This signaling mechanism may be important for TrkC-mediated cytoskeletal dynamics in podocyte disease. PMID: 29162704
  3. Rho family GTPases use the I-BAR proteins, IRSp53 (also known as BAIAP2), IRTKS and Pinkbar, as a central mechanism to modulate cell morphology. PMID: 27278019
  4. Data show that the primary effect of costimulation blockade was to decrease recruitment of the activator of actin nucleation WAVE2 (Wiskott-Aldrich syndrome protein family member protein 2) and cofilin (actin-severing protein) to F-actin. PMID: 27095595
  5. The expression of NESH/Abi-3 caused degradation of endogenous Abi-1, which led to the formation of a NESH/Abi-3-based WAVE2 complex. PMID: 26428302
  6. This study demonstrated that WAVE2-Abi2 complex, which controls growth cone activity, is crucial for completing transition from a multipolar to bipolar shape. PMID: 22617848
  7. Results of the present study show that the nucleation-promoting factors JMY and WAVE2 are critical for cytokinesis during development of mouse embryos. PMID: 23552571
  8. These results identify Wasf2 as a novel target of intermittent PTH administration via the Wnt and phosphoinositide-dependent protein kinase signaling pathways. PMID: 23525371
  9. The protein and messenger RNA levels of WAVE2 were decreased in aged oocytes, but the levels were normal in caffeine-treated aged oocytes. PMID: 22306711
  10. WAVE2 regulates oocyte polarization by regulating meiotic spindle, peripheral positioning, probably via an actin-mediated pathway, and is involved in polar body emission during mouse oocyte meiotic maturation. PMID: 21543895
  11. sential role for Abi1 in embryonic survival and WAVE2 complex integrity PMID: 21482783
  12. WAVE2 works as an A-kinase-anchoring protein that recruits PKA at membrane protrusions and plays a role in the enlargement of membrane protrusions induced by PKA activation PMID: 21119216
  13. WAVE2 colocalised with IRSp53 at the tips of protruding lamellipodia and filopodia, and the IRSp53 signal in filopodia decreased before that of WAVE2 during retraction. PMID: 12734400
  14. WAVE2 deficiency reveals distinct roles in embryogenesis and Rac-mediated actin-based motility. PMID: 12853475
  15. WAVE2-regulated actin reorganization might be required for proper cell movement and that a lack of functional WAVE2 impairs angiogenesis in vivo PMID: 12879075
  16. Results suggest that phosphatidylinositol (3,4,5)-triphosphate recruits WAVE2 to the polarized membrane and that this recruitment is essential for lamellipodium formation at the leading edge. PMID: 15107862
  17. WAVE2 acts as the primary effector downstream of Rac to achieve invasion and metastasis, suggesting that suppression of WAVE2 activity holds a promise for preventing cancer invasion and metastasis. PMID: 15608687
  18. Altogether these data identify a WAVE2-Abi1 complex crucial for the normal actin cytoskeleton reorganization and migration of macrophages in response to CSF-1. PMID: 16280551
  19. c-Abl activates WAVE2 via tyrosine phosphorylation to promote actin remodeling in vivo; Abi-1 forms the crucial link between these two factors PMID: 16899465
  20. in vitro WAVE2 is directly phosphorylated by extracellular signal-regulated kinase (ERK) 2. The proline-rich region and the verprolin, cofilin and acidic (VCA) region of WAVE2 were phosphorylated. PMID: 17202194
  21. The WAVE2/Abi1 complex differentially regulates megakaryocyte development and spreading. PMID: 17664349
  22. Phosphorylation of WAVE2 by MAP kinases regulates persistent cell migration and polarity. PMID: 18032787
  23. WAVE2-mediated actin polymerization, potentially downstream of Rac1, plays an important role in intramarrow mobilization and proliferation of hematopoietic stem cells. PMID: 19415782

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Subcellular Location
Cytoplasm, cytoskeleton. Cell projection, lamellipodium. Basolateral cell membrane.
Protein Families
SCAR/WAVE family
Database Links
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