Product Details

Purity

>85% (SDS-PAGE)

Target Names

Xbp1

Uniprot No.

O35426

Alternative Names

Xbp1; Treb5; X-box-binding protein 1; XBP-1; Tax-responsive element-binding protein 5; TREB-5) [Cleaved into: X-box-binding protein 1; cytoplasmic form; X-box-binding protein 1; luminal form]

Species

Mus musculus (Mouse)

Expression Region

1-267

Target Protein Sequence

MVVVAAAPSA ATAAPKVLLL SGQPASGGRA LPLMVPGPRA AGSEASGTPQ ARKRQRLTHL SPEEKALRRK LKNRVAAQTA RDRKKARMSE LEQQVVDLEE ENHKLQLENQ LLREKTHGLV VENQELRTRL GMDTLDPDEV PEVEAKGSGV RLVAGSAESA ALRLCAPLQQ VQAQLSPPQN IFPWTLTLLP LQILSLISFW AFWTSWTLSC FSNVLPQSLL VWRNSQRSTQ KDLVPYQPPF LCQWGPHQPS WKPLMNSFVL TMYTPSL

Protein Length

Full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Functions as a transcription factor during endoplasmic reticulum stress by regulating the unfolded protein response (UPR). Required for cardiac myogenesis and hepatogenesis during embryonic development and the development of secretory tissues such as exocrine pancreas and salivary gland. Involved in differentiation of B lymphocytes to plasma cells and production of immunoglobulins. Modulates the cellular response to ER stress in a PIK3R-dependent manner. Binds to the cis-acting X box present in the promoter regions of major histocompatibility complex class II genes. Involved in VEGF-induced endothelial cell (EC) proliferation and retinal blood vessel formation during embryonic development but also for angiogenesis in adult tissues under ischemic conditions. Functions also as a major regulator of the UPR in obesity-induced insulin resistance and type 2 diabetes for the management of obesity and diabetes prevention.; Plays a role in the unconventional cytoplasmic splicing processing of its own mRNA triggered by the endoplasmic reticulum (ER) transmembrane endoribonuclease ENR1: upon ER stress, the emerging XBP1 polypeptide chain, as part of a mRNA-ribosome-nascent chain (R-RNC) complex, cotranslationally recruits its own unprocessed mRNA through transient docking to the ER membrane and translational pausing, therefore facilitating efficient IRE1-mediated XBP1 mRNA isoform 2 production. In endothelial cells (EC), associated with KDR, promotes IRE1-mediated XBP1 mRNA isoform 2 production in a vascular endothelial growth factor (VEGF)-dependent manner, leading to EC proliferation and angiogenesis. Functions as a negative feed-back regulator of the potent transcription factor XBP1 isoform 2 protein levels through proteasome-mediated degradation, thus preventing the constitutive activation of the ER stress response signaling pathway. Inhibits the transactivation activity of XBP1 isoform 2 in myeloma cells. Acts as a weak transcriptional factor. Together with HDAC3, contributes to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI(3)K/mTORC2/Akt-dependent signaling pathway leading to EC survival under disturbed flow/oxidative stress. Binds to the ER stress response element (ERSE) upon ER stress. Binds to the consensus 5'-GATGACGTGFunctions as a stress-inducible potent transcriptional activator during endoplasmic reticulum (ER) stress by inducing unfolded protein response (UPR) target genes via binding to the UPR element (UPRE). Up-regulates target genes encoding ER chaperones and ER-associated degradation (ERAD) components to enhance the capacity of productive folding and degradation mechanism, respectively, in order to maintain the homeostasis of the ER under ER stress. Plays a role in the production of immunoglobulins and interleukin-6 in the presence of stimuli required for plasma cell differentiation, and promotes as well membrane phospholipid biosynthesis necessary for ER expansion. Contributes to the VEGF-induced endothelial cell (EC) growth and proliferation in a Akt/GSK-dependent and/or -independent signaling pathway, respectively, leading to beta-catenin nuclear translocation and E2F2 gene expression. Promotes umbilical vein EC apoptosis and atherosclerotisis development in a caspase-dependent signaling pathway, and contributes to VEGF-induced EC proliferation and angiogenesis in adult tissues under ischemic conditions. Involved in the regulation of endostatin-induced autophagy in EC through BECN1 transcriptional activation. Plays a role as an oncogene by promoting tumor progression: stimulates zinc finger protein SNAI1 transcription to induce epithelial-to-mesenchymal (EMT) transition, cell migration and invasion of breast cancer cells. Involved in adipocyte differentiation by regulating lipogenic gene expression during lactation. Plays a role in the survival of both dopaminergic neurons of the substantia nigra pars compacta (SNpc), by maintaining protein homeostasis and of myeloma cells. Increases insulin sensitivity in the liver as a response to a high carbohydrate diet, resulting in improved glucose tolerance. Improves also glucose homeostasis in an ER stress- and/or insulin-independent manner through both binding and proteasome-induced degradation of the transcription factor FOXO1, hence resulting in suppression of gluconeogenic genes expression and in a reduction of blood glucose levels. Controls the induction of de novo fatty acid synthesis in hepatocytes by regulating the expression of a subset of lipogenic genes in an ER stress- and UPR-independent manner. Binds to the 5'-CCACG-3' motif in the PPARG promoter. Associates preferentially to the HDAC3 gene promoter region in a disturbed flow-dependent manner. Binds to the BECN1 gene promoter region. Binds to the CDH5/VE-cadherin gene promoter region. Binds to the ER stress response element (ERSE) upon ER stress.

Gene References into Functions

Our findings suggest that the absence of XBP1 accelerates age-related retinal neurodegeneration PMID: 29615095
ur results demonstrate a novel and critical role of hepatic GDF15 activated by IRE1alpha-XBP1s branch in regulating adaptive responses of liver upon starvation stress. PMID: 29482168
Amyloid beta oligomers modulate BACE1 through an XBP-1-dependent pathway involving HRD1. PMID: 27853315
Results show that in beta-cells IRS1KO, there is reduced nuclear accumulation of XBP-1 due to its poor stability and proteasomal degradation. PMID: 27378176
ER stress is induced early in EAE and that modulation of ER stress by inhibition of eIF2alpha-CHOP and activation of XBP-1 in RGC specifically, protects RGC somata and axons and preserves visual function. PMID: 28726788
XBP1s transcriptionally activated Kalirin-7 (Kal7), a protein that controls synaptic plasticity. Authors found reduced levels of Kal7 in primary neurons exposed to Abeta oligomers, transgenic mouse models and human AD brains. PMID: 27646263
XBP1 deficiency in smooth muscle cells caused VSMC dedifferentiation and aggravated aortic aneurysms. XBP1u directly associated with the N terminus of FoxO4. PMID: 29089350
Data show that LPS induces endoplasmic reticulum (ER) stress and P300 activity via the XBP1/IRE1 pathway. PMID: 28743992
Data suggest that activation of GRP78/Ire1/Xbp1 pathway of ER stress-unfolded protein response is involved in mouse decidualization. PMID: 27283502
insulin and aPC converge on a common spliced-X-box binding protein-1 (sXBP1) signaling pathway to maintain endoplasmic reticulum (ER) homeostasis. PMID: 28687614
mTORC2 responds to glutamine catabolite levels to modulate the hexosamine biosynthesis enzyme GFAT1, and is essential for proper expression and nuclear accumulation of the GFAT1 transcriptional regulator, Xbp1s. PMID: 27570073
although feeding LS-Xbp1(-/-) mice cholesterol did not increase CYP7A1 expression, serum C4 levels increased significantly up to levels similar to chow-fed Xbp1(fl/fl) mice and the total bile acid pool normalized. In conclusion, loss of hepatic XBP1 decreased the bile acid pool and CYP7A1 synthetic activity. PMID: 28039331
The findings indicate that IRE1-XBP1 downregulation distinguishes germinal center B-cell-like diffuse large B-cell lymphoma (DLBCL) from other DLBCL subtypes and contributes to tumor growth. PMID: 28167662
However, depletion of XBP1 and ATF6, alone or in combination, prevented autophagy induction and significantly enhanced Japanese encephalitis virus-induced cell death. PMID: 28535855
XBP1 expression regulates the unfolded protein response, acute-phase response, and DDR in hepatocytes. In regenerating livers, XBP1 deficiency leads to endoplasmic reticulum stress and DNA damage. PMID: 28082079
Ire1alpha-Xbp1s and associated molecular targets link ER stress in arcuate Pomc neurons to aspects of normal energy and glucose homeostasis. PMID: 28028078
Overexpression of active XBP1 partially reversed 4mu8C-induced anomalies in tight junctions. PMID: 27701635
XBP1s expression in mouse and human fibroblasts is critical for TiAl6 V4 particle-induced RANKL expression and osteolysis PMID: 26403762
results reveal that XBP1s reduces hepatic lipogenic gene expression and improves hepatosteatosis in mouse models of obesity and insulin resistance, which leads us to conclude that XBP1s has anti-lipogenic properties in the liver. PMID: 27325692
Study used three experimental approaches to enhance the IKKbeta activity in the liver of obese mice and observed increased XBP1s activity, reduced endoplasmic reticulum stress, and a significant improvement in insulin sensitivity and consequently in glucose homeostasis. PMID: 27814504
Findings indicate that PERK kinase-activating transcription factor 4 (ATF4) pathway affected the efficiency of X-box binding protein 1 (XBP1) mRNA splicing by regulating inositol-requiring enzyme 1alpha (IRE1alpha) expression. PMID: 27052593
Gene expression analysis revealed that XBP1 regulates a group of memory-related genes, highlighting BDNF, as a key component in memory consolidation. PMID: 26854229
Data shsow that X-box binding protein 1 (XBP1) plays a critical role in controlling the oxidative stress in Leishmania amazonensis a-infected cells. PMID: 26678450
Diminished XBP1 and/or HNF4alpha in beta-cells led to impaired endoplasmic reticulum calcium homeostasis. PMID: 26792861
propose that the pathology of MCDS is underpinned by XBP1 independent UPR-induced dysregulation of C/EBP-beta-mediated chondrocyte differentiation PMID: 26372225
Data (including data from studies in knockout/transgenic mice) Xbp1 is required in mammary epithelial cells during lactation for cell proliferation (vs. apoptosis) and for development of elaborate endoplasmic reticulum (ER; without ER stress). PMID: 26562262
data indicate that hepatic XBP1 controls the adaptive response of the UPR and is critical to restoring homeostasis in the liver in response to ER stress. PMID: 26504083
Loss of XBP1 enhances liver injury in mice fed a high fat/sugar diet. PMID: 26472223
findings suggest that the balance between XBP1-mediated adaptive and CHOP-dependent apoptotic UPR is critically important for beta-cell survival during ER stress. PMID: 26135354
The findings reported herein indicate that palmitate-induced cisternal endoplasmic reticulum expansion is dependent on the activation of XBP-1/CCTalpha-mediated phospholipid accumulation in RAW 264.7 cells. PMID: 26174230
Endothelial XBP-1 is activated by TNF and regulates leukocyte-endothelial cell adhesion in vitro as well as neutrophil infiltration and vascular damage in murine vasculitis. PMID: 26245636
These results suggest that aminoglycoside-induced endoplasmic reticulum stress and cell death in spiral ganglion neurons is mitigated by XBP1, masking aminoglycoside neurotoxicity at the organismal level. PMID: 25973683
Defective podocyte insulin signaling through p85-XBP1 promotes ATF6-dependent maladaptive ER-stress response in diabetic nephropathy. PMID: 25754093
Our data indicate that removal of XBP-1 confers a kinetic delay in early stages of MCMV infection and suggest that the late targeting of IRE1 is aimed at inhibiting activities other than the splicing of XBP-1 mRNA PMID: 25333725
XBP1 expression plays an important role in the neointima formation in vascular injuries. PMID: 26315405
Enhanced splicing of XBP-1 was observed in BCL-2 overexpressing cells implicating BCL-2 in the complex regulation of IRE1alpha activity. PMID: 26319553
XBP1 plays a role in regulating chondrocyte proliferation and the timing of mineralization during endochondral ossification. PMID: 25600960
results demonstrate that XBP1 mRNA splicing plays an important role in maintaining the function of bone marrow-derived macrophages and provide new insight into the study and treatment of atherosclerosis PMID: 26026678
The present study thus reveals an intracellular pathway that integrates the UPR and osteoclast differentiation through activation of the IRE1alpha/XBP1 pathway. PMID: 26193638
Data indicate that the transcription factor XBP1 is indispensable for eosinophil production. PMID: 26147683
Increased endoplasmic reticulum stress stress contributes to neointima formation after vascular injury, while unfolded protein response signaling downstream of XBP1 plays a suppressive role. PMID: 25373918
These results indicate that IRE1alpha can promote beta-cell proliferation through, at least in part, XBP1s upregulation of cyclin D1, which is known to drive cells from the G1 into the S-phase of the cell cycle. PMID: 24797433
SEC63 function regulates IRE1alpha/XBP1 activation. SEC63 and XBP1 are required for GPS cleavage and maturation of PC1. Activation of XBP1 can protect against polycystic disease in the setting of impaired biogenesis of PC1. PMID: 25844898
these findings suggest that miR-214 is an important regulator of angiogenesis in heart in vitro and in vivo, likely via regulating the expression of XBP1 PMID: 25656649
The loss of Xbp1 induces a terminal unfolded protein response which blocks proliferation and differentiation during mammary gland development. PMID: 25713103
Endoplasmic reticulum stress-induced IRE1alpha activation mediates cross-talk of GSK-3beta and XBP-1 to regulate inflammatory cytokine production. PMID: 25821218
The IRE1alpha-XBP1 pathway of the unfolded protein response could directly activate the transcriptional expression of Fgf21. PMID: 25170079
that XBP1 is an important gene involved in regulation of the anti-oxidant defense in the RPE, and that impaired activation of XBP1 may contribute to RPE dysfunction and cell death during retinal degeneration and AMD. PMID: 22715395
mTOR has a role in plasma cell differentiation and bypasses XBP-1 for immunoglobulin secretion PMID: 25332234
Positive regulation of PPARG2 is a principal mechanism of XBP1s-mediated adipogenesis in 3T3-L1 cells. PMID: 25223794

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Subcellular Location

Endoplasmic reticulum.; [Isoform 1]: Nucleus. Cytoplasm. Endoplasmic reticulum membrane; Single-pass type II membrane protein. Endoplasmic reticulum membrane; Peripheral membrane protein. Membrane; Peripheral membrane protein.; [Isoform 2]: Nucleus. Cytoplasm.; [X-box-binding protein 1, cytoplasmic form]: Cytoplasm. Nucleus.

Protein Families

BZIP family

Tissue Specificity

Isoform 1 and isoform 2 are expressed at higher level in branch curves of vessel walls and in atherosclerotic plaques relative to healthy segments of the same aortas (at protein level). Expressed in skeletal muscles, plasma cells and pancreatic beta cells

Database Links

KEGG: mmu:22433

STRING: 10090.ENSMUSP00000054852

UniGene: Mm.469937

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Code	CSB-YP026183MO
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Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-MP026183MO
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Recombinant Mouse X-box-binding protein 1 (Xbp1)

Product Details

Customer Reviews and Q&A

Target Background

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