Recombinant Mouse Zinc finger protein 42 (Zfp42)

Code CSB-YP026453MO
MSDS
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Source Yeast
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Code CSB-EP026453MO
MSDS
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Source E.coli
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Code CSB-EP026453MO-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP026453MO
MSDS
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Source Baculovirus
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Code CSB-MP026453MO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Zfp42
Uniprot No.
Alternative Names
Zfp42; Rex-1; Rex1Zinc finger protein 42; Zfp-42; Reduced expression protein 1; REX-1; mREX-1
Species
Mus musculus (Mouse)
Expression Region
1-288
Target Protein Sequence
MNEQKMNEQM KKTAKTSGQK GPGGRALDRL TLKQDEARPV QNTRVEAPRV TYTIRDESEI SPETEEDGFP DGYLECIIRG EFSEPILEED FLFKSFESLE EVEQNLSRQV LEASSLLESS LEYMTKGTKQ EKTEVTQETP PLRVGASSLL AGGPAEKPEG GVYCGVLSML ECPQAGCKKK LRGKTALRKH MLVHGPRRHV CAECGKAFTE SSKLKRHFLV HTGEKPYQCT FEGCGKRFSL DFNLRTHIRI HTGERRFVCP FDGCEKSFIQ SNNQKIHILT HAKAGKKC
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency. Required for efficient elongation of TSIX, a non-coding RNA antisense to XIST. Binds DXPas34 enhancer within the TSIX promoter. Involved in ES cell self-renewal.
Gene References into Functions
  1. Data show that zinc finger protein 42 (Rex1) and zinc finger and SCAN domain containing 4D/4C (Zscan4D/4C) correlate with the cell-cycle length under different mechanisms. PMID: 26997646
  2. Results show that a novel pluripotency regulator, REX1, is essential for pluripotency and reprogramming. PMID: 23939908
  3. Rex1 plays a role during preimplantation development and alters expression levels of Zscan4. PMID: 22897771
  4. Results show that Rex1 regulates endogenous retroviral element expression in mouse embryonic stem cells and during pre-implantation development and suggest that Rex1 and its relatives have evolved as regulators of endogenous retroviral transcription. PMID: 22844087
  5. RNF12 causes REX1 breakdown through dose-dependent catalysis, thereby representing an important pathway to initiate X-chromosome inactivation PMID: 22596162
  6. the involvement of Rex-1 in control of Polycomb target genes during pluripotency or differentiation. PMID: 21530438
  7. loss of Rex1 leads to impaired testicular function. PMID: 21641340
  8. the functional connection of Rex1 to genomic imprinting represents another case where newly made genes have co-evolved with lineage-specific phenomena. PMID: 21233130
  9. Rex-1 transcription factor regulates the differentiation of F9 stem cells along several distinct cell lineages found in the early embryo. PMID: 12354678
  10. Embryonic stem cell marker Rex-1 and the pluripotent stem cell marker Oct-4 are spontaneously expressed by untreated mesenchymal stem cells. PMID: 16793732
  11. Rex-1 marker was assessed using flow cytometry and gene expression profiling. PMID: 17518637
  12. Rex1 should be regarded just as a marker of pluripotency without functional significance PMID: 18433507
  13. The disruption of the Rex1 gene enhanced the expression of ectoderm, mesoderm, and endoderm markers as compared to wild-type embryonic stem cells PMID: 19618472

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Subcellular Location
Nucleus.
Protein Families
Krueppel C2H2-type zinc-finger protein family
Tissue Specificity
Restricted to testis, to germ cells in the early stages of spermatogenesis. Not expressed in spermatids, nor spermatozoa. Expressed in embryonic stem (ES) cells.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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