Recombinant Mouse Zona pellucida sperm-binding protein 2 (Zp2), partial

Code CSB-YP027120MO1
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Source Yeast
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Code CSB-EP027120MO1
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Source E.coli
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Code CSB-EP027120MO1-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP027120MO1
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Source Baculovirus
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Code CSB-MP027120MO1
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Zp2
Uniprot No.
Alternative Names
Zp2; Zp-2; Zpa; Zona pellucida sperm-binding protein 2; Zona pellucida glycoprotein 2; Zona pellucida protein A
Species
Mus musculus (Mouse)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of the zona pellucida, an extracellular matrix surrounding oocytes which mediates sperm binding, induction of the acrosome reaction and prevents post-fertilization polyspermy. The zona pellucida is composed of 3 to 4 glycoproteins, ZP1, ZP2, ZP3, and ZP4. ZP2 may act as a secondary sperm receptor.
Gene References into Functions
  1. The central peptide of ZP2 (190-505) is immunogenic and contains zona pellucida-specific epitopes. PMID: 27193969
  2. ZP2(51-149) sperm-binding domain is necessary for mouse gamete recognition and penetration through the zona pellucida. PMID: 24934154
  3. Studies indicate that eggs from mZP2-/- and mZP3-/- females lack a zona pellucida (ZP), and the mice are completely infertile due to a severely reduced number of ovulated eggs in their oviducts. PMID: 23417405
  4. Sperm arylsulfatase A binds to mZP2 and mZP3 glycoproteins in a nonenzymatic manner. PMID: 22685254
  5. Recombinant ovastacin cleaved ZP2 in native zonae pellucidae, documenting that ZP2 was a direct substrate of this metalloendoprotease. PMID: 22472438
  6. Cytoplasmic tails are necessary to prevent intracellular oligomerization while ensuring incorporation of processed ZP2 and ZP3 into the zona pellucida. PMID: 21378311
  7. The granulosa cells contribute more to the mZP2 mRNA synthesis than oocyte does. PMID: 20979237
  8. data indicate that sperm-egg recognition depends on the cleavage status of ZP2 and that binding at the surface of the zona is not sufficient to induce sperm acrosome exocytosis PMID: 20616279
  9. Results indicate that ZP2 and ZP3 traffic independently through the oocyte prior to assembly into the zona pellucida. PMID: 17047254

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Subcellular Location
[Processed zona pellucida sperm-binding protein 2]: Zona pellucida.; Cell membrane; Single-pass type I membrane protein.
Protein Families
ZP domain family, ZPA subfamily
Tissue Specificity
Expressed in oocytes (at protein level).
Database Links
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