Recombinant Musca domestica Polycomb protein esc (esc)

Code CSB-YP519413MVC
MSDS
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Source Yeast
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Code CSB-EP519413MVC
MSDS
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Source E.coli
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Code CSB-EP519413MVC-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP519413MVC
MSDS
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Source Baculovirus
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Code CSB-MP519413MVC
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
esc
Uniprot No.
Alternative Names
escPolycomb protein esc; Protein extra sex combs
Species
Musca domestica (House fly)
Expression Region
1-428
Target Protein Sequence
MSKVKADKQS PKENTKAENS DNESIDDTAS VATSTTSRSK SPGSRGRRQS RGHSKSKAKR PAYKYDCHLK EDHGQAIFGV SFNHLLGKDQ SMVFATAGSN RCNIYECPRK GGLKLIMCYA DPDPDEVFYT CSWSYDLKTS APLLATAGYR GVIRVIDIHR NESVGNYVGH GQAINELKFH PRQANLLLSG SKDHAIRLWN IQTHVCIAIF GGVEGHRDEV LSIDFDLRGE RIMSSGMDHS LKLWRIDTPE FKDKIEMSRT FNPNKSQLPF PTIMQHFPEF STRDIHRNYV DCVQWFGDFV LSKSCENSIV CWKPGQLHQT LSQLKPNDPS CTIISEFNYD ECEIWFVRFG FNPWHKIVAL GNQYGKVYVW ELDPSDPRHT HSSTLNNIRC TSIVRQTAFS RDATVLVWVC DDGTVWRWNR RNAEVHTP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Polycomb group (PcG) protein. In contrast to other PcG protein, it is specifically required during the first 6 hours of embryogenesis to establish PcG silencing. PcG proteins act by forming multiprotein complexes, which are required to maintain the transcriptionally repressive state of homeotic genes throughout development. PcG proteins are not required to initiate repression, but to maintain it during later stages of development. They probably act via a methylation of histones, rendering chromatin heritably changed in its expressibility. Component of the Esc/E(z) complex, which methylates 'Lys-9' and 'Lys-27' residues of histone H3. The Esc/E(z) complex is necessary but not sufficient to recruit a functional PcG repressive complex that represses target genes, suggesting that the recruitment of the distinct PRC1 complex is also required to allow a subsequent repression.
Subcellular Location
Nucleus.
Protein Families
WD repeat ESC family
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