Recombinant Mycobacterium smegmatis FAD-containing monooxygenase EthA (ethA)

Code CSB-YP368062MVX
MSDS
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Source Yeast
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Code CSB-EP368062MVX
MSDS
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Source E.coli
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Code CSB-EP368062MVX-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP368062MVX
MSDS
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Source Baculovirus
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Code CSB-MP368062MVX
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ethA
Uniprot No.
Alternative Names
ethA; MSMEG_6440; MSMEI_6272FAD-containing monooxygenase EthA; EC 1.14.13.-; Baeyer-Villiger monooxygenase; BVMO; Prodrug activator EtaA
Species
Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Expression Region
1-492
Target Protein Sequence
MTEHFDVVIV GAGISGISTA WHLQDRCPTK SYVILERRAN IGGTWDLFKY PGIRSDSDMF TLGFRFKPWT SAKSIADGPS IWNYINEAAQ ENGIDKHIRT NHRVLGADWS DAENRWTITV EADGEQKQIT ASFLSVCSGY YNYDQGYSPE FPGADDFAGQ IIHPQHWPED LDYAGKKIVV IGSGATAVTL IPSLVNGGAA HVTMLQRSPT YIGSLPLVDP VAEKTNKYLP KNLAHFVNRW KAIAFSTAQY QLARKFPNYM RKTLMTMAQR RLPEGFDVQK HFGPRYNPWD ERLCLAPNGD LFKTIRAGKA DVVTDTIAKF TETGIKLTSG EELTADIIIT ATGLNMQLFG GASLTRNGQE VDLTETMTYK GLMLSGVPNM AITFGYTNAS WTLKADLVSE FICRVLNYMD DNGFDRVEPQ HPGDAVDALP FMDFNPGYFR RAMDSLPKSG SRAPWRLKQN YFFDLRMIRY DKVDEESLHF TKHRAAVSAS SS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Monooxygenase able to convert a wide range of ketones to the corresponding esters or lactones via a Baeyer-Villiger oxidation reaction. Can act on long-chain aliphatic ketones (2-hexanone to 2-dodecanone) and on aromatic ketones (phenylacetone and benzylacetone). Is also able to catalyze enantioselective sulfoxidation of methyl-p-tolylsulfide. In vivo, likely functions as a BVMO, but the exact nature of the physiological substrate(s) remains to be established.; Is responsible for the activation of several thiocarbamide-containing pro-drugs, such as ethionamide (ETH), isoxyl (ISO) and thiacetazone (TAC), into reactive species.
Subcellular Location
Cell membrane.
Protein Families
FAD-binding monooxygenase family
Database Links
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