Recombinant Mycobacterium smegmatis Glutamate--cysteine ligase GshA(gshA)

Product Type Recombinant Protein
Uniprot No. A0R5N1
Species Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Gene Names egtA
Protein Names Recommended name: Glutamate--cysteine ligase GshA EC= 6.3.2.2 Alternative name(s): Gamma-ECS Short name= GCS Gamma-glutamylcysteine synthetase
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Catalyzes the synthesis of gamma-glutamylcysteine (gamma-GC) (By similarity). This compound is used as substrate for the biosynthesis of the low-molecular thiol compound ergothioneine
Protein Families Glutamate--cysteine ligase type 2 family, EgtA subfamily
Database Links

KEGG: msb:LJ00_30905

STRING: 246196.MSMEG_6250

Product Details for Different Expression Systems

Product Name Recombinant Mycobacterium smegmatis Glutamate--cysteine ligase GshA(gshA),Yeast
Code CSB-YP368057MVX
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MALPARSDSG CAVPVEFTSA EQAAAHIGAN SLQDGPIGRV GLEIEAHCFD LSNPTRRPSW DELSAVIADV PPLPGGSRIT VEPGGAVELS GPPYDGPLAA VAALQADRAV LRAEFARRNL GLVLLGTDPL RPTRRVNPGA RYSAMEQFFT ASGTAEAGAA MMTATASVQV NLDAGPRDGW AERVRLAHAL GPTMIAITAN SPMLGGQFTG WCSTRQRVWG QLDSARCGPV LGVDGDDPAS EWARYALRAP VMLVNSPDAV PVTNWVPFAD WADGRAVLGG RRPTEADLDY HLTTLFPPVR PRRWLEIRYL DSVPDALWPA AVFTLTTLLD DPVAAESAAE ATRPVATAWD RAARMGLTDR HLHTAALTCV RLAAERAPAE LEESMTLLMR SVQQRRSPAD DFSDRVVARG IAAAVRELAK GEL
Expression Region 1-423
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Mycobacterium smegmatis Glutamate--cysteine ligase GshA(gshA),E.coli
Code CSB-EP368057MVX
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MALPARSDSG CAVPVEFTSA EQAAAHIGAN SLQDGPIGRV GLEIEAHCFD LSNPTRRPSW DELSAVIADV PPLPGGSRIT VEPGGAVELS GPPYDGPLAA VAALQADRAV LRAEFARRNL GLVLLGTDPL RPTRRVNPGA RYSAMEQFFT ASGTAEAGAA MMTATASVQV NLDAGPRDGW AERVRLAHAL GPTMIAITAN SPMLGGQFTG WCSTRQRVWG QLDSARCGPV LGVDGDDPAS EWARYALRAP VMLVNSPDAV PVTNWVPFAD WADGRAVLGG RRPTEADLDY HLTTLFPPVR PRRWLEIRYL DSVPDALWPA AVFTLTTLLD DPVAAESAAE ATRPVATAWD RAARMGLTDR HLHTAALTCV RLAAERAPAE LEESMTLLMR SVQQRRSPAD DFSDRVVARG IAAAVRELAK GEL
Expression Region 1-423
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Mycobacterium smegmatis Glutamate--cysteine ligase GshA(gshA),Baculovirus
Code CSB-BP368057MVX
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MALPARSDSG CAVPVEFTSA EQAAAHIGAN SLQDGPIGRV GLEIEAHCFD LSNPTRRPSW DELSAVIADV PPLPGGSRIT VEPGGAVELS GPPYDGPLAA VAALQADRAV LRAEFARRNL GLVLLGTDPL RPTRRVNPGA RYSAMEQFFT ASGTAEAGAA MMTATASVQV NLDAGPRDGW AERVRLAHAL GPTMIAITAN SPMLGGQFTG WCSTRQRVWG QLDSARCGPV LGVDGDDPAS EWARYALRAP VMLVNSPDAV PVTNWVPFAD WADGRAVLGG RRPTEADLDY HLTTLFPPVR PRRWLEIRYL DSVPDALWPA AVFTLTTLLD DPVAAESAAE ATRPVATAWD RAARMGLTDR HLHTAALTCV RLAAERAPAE LEESMTLLMR SVQQRRSPAD DFSDRVVARG IAAAVRELAK GEL
Expression Region 1-423
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Mycobacterium smegmatis Glutamate--cysteine ligase GshA(gshA),Mammalian cell
Code CSB-MP368057MVX
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MALPARSDSG CAVPVEFTSA EQAAAHIGAN SLQDGPIGRV GLEIEAHCFD LSNPTRRPSW DELSAVIADV PPLPGGSRIT VEPGGAVELS GPPYDGPLAA VAALQADRAV LRAEFARRNL GLVLLGTDPL RPTRRVNPGA RYSAMEQFFT ASGTAEAGAA MMTATASVQV NLDAGPRDGW AERVRLAHAL GPTMIAITAN SPMLGGQFTG WCSTRQRVWG QLDSARCGPV LGVDGDDPAS EWARYALRAP VMLVNSPDAV PVTNWVPFAD WADGRAVLGG RRPTEADLDY HLTTLFPPVR PRRWLEIRYL DSVPDALWPA AVFTLTTLLD DPVAAESAAE ATRPVATAWD RAARMGLTDR HLHTAALTCV RLAAERAPAE LEESMTLLMR SVQQRRSPAD DFSDRVVARG IAAAVRELAK GEL
Expression Region 1-423
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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