Recombinant Mycobacterium smegmatis Holliday junction ATP-dependent DNA helicase RuvA(ruvA)

Product Type Recombinant Protein
Uniprot No. A0QWH5
Species Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
Gene Names ruvA
Protein Names Recommended name: Holliday junction ATP-dependent DNA helicase RuvA EC= 3.6.4.12
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function The RuvA-RuvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is a helicase that mediates the Holliday junction migration by localized denaturation and reannealing. RuvA stimulates, in the presence of DNA, the weak ATPase activity of RuvB.
Protein Families RuvA family
Database Links

KEGG: msb:LJ00_14650

STRING: 246196.MSMEG_2944

Product Details for Different Expression Systems

Product Name Recombinant Mycobacterium smegmatis Holliday junction ATP-dependent DNA helicase RuvA(ruvA),Yeast
Code CSB-YP368002MVX
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MIASVRGEVI DIALDHAVIE AAGVGYKVMA TPSTLATLRR GTEARLITAM IVREDSMTLY GFVDGDARDL FLTLLGVSGV GPKIALATLA VYDPQALRQA LADGDVTALT RVPGIGKRGA ERMVLELRDK IGPVSAGGGA AVGGHAIRGP VVEALVGLGF AAKQAEEATD KVLANDPEAT TSSALRAALS MLGKK
Expression Region 1-195
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
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Product Name Recombinant Mycobacterium smegmatis Holliday junction ATP-dependent DNA helicase RuvA(ruvA),E.coli
Code CSB-EP368002MVX
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MIASVRGEVI DIALDHAVIE AAGVGYKVMA TPSTLATLRR GTEARLITAM IVREDSMTLY GFVDGDARDL FLTLLGVSGV GPKIALATLA VYDPQALRQA LADGDVTALT RVPGIGKRGA ERMVLELRDK IGPVSAGGGA AVGGHAIRGP VVEALVGLGF AAKQAEEATD KVLANDPEAT TSSALRAALS MLGKK
Expression Region 1-195
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Mycobacterium smegmatis Holliday junction ATP-dependent DNA helicase RuvA(ruvA),Baculovirus
Code CSB-BP368002MVX
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MIASVRGEVI DIALDHAVIE AAGVGYKVMA TPSTLATLRR GTEARLITAM IVREDSMTLY GFVDGDARDL FLTLLGVSGV GPKIALATLA VYDPQALRQA LADGDVTALT RVPGIGKRGA ERMVLELRDK IGPVSAGGGA AVGGHAIRGP VVEALVGLGF AAKQAEEATD KVLANDPEAT TSSALRAALS MLGKK
Expression Region 1-195
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Mycobacterium smegmatis Holliday junction ATP-dependent DNA helicase RuvA(ruvA),Mammalian cell
Code CSB-MP368002MVX
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MIASVRGEVI DIALDHAVIE AAGVGYKVMA TPSTLATLRR GTEARLITAM IVREDSMTLY GFVDGDARDL FLTLLGVSGV GPKIALATLA VYDPQALRQA LADGDVTALT RVPGIGKRGA ERMVLELRDK IGPVSAGGGA AVGGHAIRGP VVEALVGLGF AAKQAEEATD KVLANDPEAT TSSALRAALS MLGKK
Expression Region 1-195
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat

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