Recombinant Nitrobacter hamburgensis (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB(miaB)

Product Type Recombinant Protein
Uniprot No. Q1QS74
Abbreviation miaB
Species Nitrobacter hamburgensis (strain DSM 10229 / NCIMB 13809 / X14)
Gene Names miaB
Protein Names Recommended name: (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB EC= 2.-.-.- Alternative name(s): tRNA-i(6)A37 methylthiotransferase
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Catalyzes the methylthiolation of N6-(dimethylallyl)adenosine (i(6)A), leading to the formation of 2-methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine.
Subcellular Location Cytoplasm
Protein Families Methylthiotransferase family, MiaB subfamily
Database Links

KEGG: nha:Nham_0021

STRING: 323097.Nham_0021

Product Details for Different Expression Systems

Product Name Recombinant Nitrobacter hamburgensis (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB(miaB),Yeast
Code CSB-YP629985NAAF
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MTPPRKLHIK SYGCQMNVYD AQRMVDTLAA EGFVETASAE EADLVILNTC HIREKASEKV YSELGRLRAA KDQAARDGRE MSVVVAGCVA QAEGDEIIRR APVVDVVVGP QSYHHLPQLL ARAKADGRAL ETEFPVEDKF GFLPQPSRQA IRARGISAFV TVQEGCDKFC TFCVVPYTRG AEVSRPVAKI VEDVQRLADN GVREITLIGQ NVNAYHGDGP DGRPWPFGKL LHRLADIPGI VRLRYSTSHP RDVEDSLIEA HRDLGALMPF VHLPVQSGSD RILEAMNRRH TADDYRRVVD RFRHVRQDIA FSSDFIVGFP GETEQDFTAT LALVTQIGYA GAYSFKYSPR PGTPAAEMKE TVSTADMDER LVRLQNLIDS QQSAFNRAAV GTTVDVLFER AARNPGQIVG RTAYLQPAHV VASADIIGQI LPVTVASLER YSLLGSLASA PASRASADDA PPVGASSPAI MGV
Expression Region 1-473
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
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Product Name Recombinant Nitrobacter hamburgensis (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB(miaB),E.coli
Code CSB-EP629985NAAF
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MTPPRKLHIK SYGCQMNVYD AQRMVDTLAA EGFVETASAE EADLVILNTC HIREKASEKV YSELGRLRAA KDQAARDGRE MSVVVAGCVA QAEGDEIIRR APVVDVVVGP QSYHHLPQLL ARAKADGRAL ETEFPVEDKF GFLPQPSRQA IRARGISAFV TVQEGCDKFC TFCVVPYTRG AEVSRPVAKI VEDVQRLADN GVREITLIGQ NVNAYHGDGP DGRPWPFGKL LHRLADIPGI VRLRYSTSHP RDVEDSLIEA HRDLGALMPF VHLPVQSGSD RILEAMNRRH TADDYRRVVD RFRHVRQDIA FSSDFIVGFP GETEQDFTAT LALVTQIGYA GAYSFKYSPR PGTPAAEMKE TVSTADMDER LVRLQNLIDS QQSAFNRAAV GTTVDVLFER AARNPGQIVG RTAYLQPAHV VASADIIGQI LPVTVASLER YSLLGSLASA PASRASADDA PPVGASSPAI MGV
Expression Region 1-473
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Nitrobacter hamburgensis (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB(miaB),Baculovirus
Code CSB-BP629985NAAF
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MTPPRKLHIK SYGCQMNVYD AQRMVDTLAA EGFVETASAE EADLVILNTC HIREKASEKV YSELGRLRAA KDQAARDGRE MSVVVAGCVA QAEGDEIIRR APVVDVVVGP QSYHHLPQLL ARAKADGRAL ETEFPVEDKF GFLPQPSRQA IRARGISAFV TVQEGCDKFC TFCVVPYTRG AEVSRPVAKI VEDVQRLADN GVREITLIGQ NVNAYHGDGP DGRPWPFGKL LHRLADIPGI VRLRYSTSHP RDVEDSLIEA HRDLGALMPF VHLPVQSGSD RILEAMNRRH TADDYRRVVD RFRHVRQDIA FSSDFIVGFP GETEQDFTAT LALVTQIGYA GAYSFKYSPR PGTPAAEMKE TVSTADMDER LVRLQNLIDS QQSAFNRAAV GTTVDVLFER AARNPGQIVG RTAYLQPAHV VASADIIGQI LPVTVASLER YSLLGSLASA PASRASADDA PPVGASSPAI MGV
Expression Region 1-473
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Nitrobacter hamburgensis (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB(miaB),Mammalian cell
Code CSB-MP629985NAAF
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MTPPRKLHIK SYGCQMNVYD AQRMVDTLAA EGFVETASAE EADLVILNTC HIREKASEKV YSELGRLRAA KDQAARDGRE MSVVVAGCVA QAEGDEIIRR APVVDVVVGP QSYHHLPQLL ARAKADGRAL ETEFPVEDKF GFLPQPSRQA IRARGISAFV TVQEGCDKFC TFCVVPYTRG AEVSRPVAKI VEDVQRLADN GVREITLIGQ NVNAYHGDGP DGRPWPFGKL LHRLADIPGI VRLRYSTSHP RDVEDSLIEA HRDLGALMPF VHLPVQSGSD RILEAMNRRH TADDYRRVVD RFRHVRQDIA FSSDFIVGFP GETEQDFTAT LALVTQIGYA GAYSFKYSPR PGTPAAEMKE TVSTADMDER LVRLQNLIDS QQSAFNRAAV GTTVDVLFER AARNPGQIVG RTAYLQPAHV VASADIIGQI LPVTVASLER YSLLGSLASA PASRASADDA PPVGASSPAI MGV
Expression Region 1-473
Protein Description full length protein
Storage Buffer Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Still Have Questions? Leave a Message or Start an on-line Chat

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