Recombinant Pig Dehydrogenase/reductase SDR family member 4 (DHRS4)

Code CSB-YP840964PI
MSDS
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Source Yeast
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Code CSB-EP840964PI
MSDS
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Source E.coli
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Code CSB-EP840964PI-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP840964PI
MSDS
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Source Baculovirus
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Code CSB-MP840964PI
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DHRS4
Uniprot No.
Alternative Names
DHRS4Dehydrogenase/reductase SDR family member 4; EC 1.1.1.184; NADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase; CR; PHCR; NADPH-dependent retinol dehydrogenase/reductase; NDRD; Peroxisomal short-chain alcohol dehydrogenase; PSCD
Species
Sus scrofa (Pig)
Expression Region
1-279
Target Protein Sequence
MRAAGQLLRA CSQTWKSVRM ASTGVERRKP LENKVALVTA STDGIGLAIA RRLAQDGAHV VVSSRKQENV DRTVATLQGE GLSVTGTVCH VGKAEDRERL VAMAVNLHGG VDILVSNAAV NPFFGNIIDA TEEVWDKILH VNVKATVLMT KAVVPEMEKR GGGSVLIVSS VGAYHPFPNL GPYNVSKTAL LGLTKNLAVE LAPRNIRVNC LAPGLIKTNF SQVLWMDKAR KEYMKESLRI RRLGNPEDCA GIVSFLCSED ASYITGETVV VGGGTASRL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Reduces all-trans-retinal and 9-cis retinal. Can also catalyze the oxidation of all-trans-retinol with NADP as co-factor, but with much lower efficiency. Reduces alkyl phenyl ketones and alpha-dicarbonyl compounds with aromatic rings, such as pyrimidine-4-aldehyde, 3-benzoylpyridine, 4-benzoylpyridine, menadione and 4-hexanoylpyridine. Has no activity towards aliphatic aldehydes and ketones.
Gene References into Functions
  1. Studied the association of postmortem pH with SNPs in the fourth member of the short-chain dehydrogenase/reductase family (DHRS4), the first member of serpin peptidase inhibitor, clade G (complement inhibitor) (SERPING1), and the apolipoprotein R precursor (APOR) genes in Berkshire pigs. Results suggest that DHRS4, SERPING1, and APOR snps may be potential genetic markers for meat quality. PMID: 28489967
  2. Sus scrofa DHRS4 gene is ortholog of human DHRS4 gene. These two genes have identical splicing phases, exon-intron structure and their coded proteins have identical catalytic amino-acid residues, Rossman fold consensus, Peroxisomal Targeting Signal consensus and the three amino-acid residues typical of vertebrate DHRS4 enzymes PMID: 23036705
  3. Peroxisomal localization of tetrameric carbonyl reductase. PMID: 18334214
Subcellular Location
Peroxisome.
Protein Families
Short-chain dehydrogenases/reductases (SDR) family
Tissue Specificity
Detected in heart, kidney, liver and small intestine. Detected at lower levels in brain, lung, stomach and spleen.
Database Links

KEGG: ssc:397082

STRING: 9823.ENSSSCP00000002200

UniGene: Ssc.86

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