Recombinant Pig Myosin light chain kinase, smooth muscle(MYLK)

Product Type Recombinant Protein
Uniprot No. P79280
Species Sus scrofa (Pig)
Gene Names MYLK
Protein Names Recommended name: Myosin light chain kinase, smooth muscle Short name= MLCK EC= 2.7.11.18
Storage Store at -20°C, for extended storage, conserve at -20°C or -80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Target Details This gene, a muscle member of the immunoglobulin gene superfamily, encodes myosin light chain kinase which is a calcium/calmodulin dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3 region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.
HGNC 7590
RGD 1310915
MGI 894806
Function Phosphorylates a specific serine in the N-terminus of a myosin light chain. Also regulates actin-myosin interaction through a non-kinase activity (By similarity).
Protein Families Protein kinase superfamily, CAMK Ser/Thr protein kinase family
Database Links

STRING: 9823.ENSSSCP00000012639

UniGene: Ssc.16164

Pathway Calcium signaling pathway
Focal adhesion
Regulation of actin cytoskeleton
Vascular smooth muscle contraction
Apelin signaling pathway
cGMP-PKG signaling pathway

Product Details for Different Expression Systems

Product Name Recombinant Pig Myosin light chain kinase, smooth muscle(MYLK),Yeast
Code CSB-YP015320PI
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence FIVLSQEGSL CSISIEKALP EDRGLYKCVA KNSAGQAECS CQVTVDDAPT GENAKAPEMK ARRPKSSLPP VLGTESDATV KKKPAPKTPP KAAMPPQIIQ FPEDQKVRAG ESVELFGKVA GTQPITCTWM KFRKQIQES
Expression Region 1-139
Protein Description Full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Pig Myosin light chain kinase, smooth muscle(MYLK),E.coli
Code CSB-EP015320PI
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence FIVLSQEGSL CSISIEKALP EDRGLYKCVA KNSAGQAECS CQVTVDDAPT GENAKAPEMK ARRPKSSLPP VLGTESDATV KKKPAPKTPP KAAMPPQIIQ FPEDQKVRAG ESVELFGKVA GTQPITCTWM KFRKQIQES
Expression Region 1-139
Protein Description Full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Pig Myosin light chain kinase, smooth muscle(MYLK),Baculovirus
Code CSB-BP015320PI
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence FIVLSQEGSL CSISIEKALP EDRGLYKCVA KNSAGQAECS CQVTVDDAPT GENAKAPEMK ARRPKSSLPP VLGTESDATV KKKPAPKTPP KAAMPPQIIQ FPEDQKVRAG ESVELFGKVA GTQPITCTWM KFRKQIQES
Expression Region 1-139
Protein Description Full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Pig Myosin light chain kinase, smooth muscle(MYLK),Mammalian cell
Code CSB-MP015320PI
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence FIVLSQEGSL CSISIEKALP EDRGLYKCVA KNSAGQAECS CQVTVDDAPT GENAKAPEMK ARRPKSSLPP VLGTESDATV KKKPAPKTPP KAAMPPQIIQ FPEDQKVRAG ESVELFGKVA GTQPITCTWM KFRKQIQES
Expression Region 1-139
Protein Description Full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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