Recombinant Polynucleobacter sp. UPF0173 metal-dependent hydrolase Pnuc_1524 (Pnuc_1524)

Code CSB-YP391864PYU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP391864PYU
MSDS
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Source E.coli
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Code CSB-EP391864PYU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP391864PYU
MSDS
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Source Baculovirus
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Code CSB-MP391864PYU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pnuc_1524
Uniprot No.
Alternative Names
Pnuc_1524; UPF0173 metal-dependent hydrolase Pnuc_1524
Species
Polynucleobacter asymbioticus (strain DSM 18221 / CIP 109841 / QLW-P1DMWA-1) (Polynucleobacter necessarius subsp. asymbioticus)
Expression Region
1-285
Target Protein Sequence
MVNILKKRAF GGAILAALLL ASSAYVGAQS APAAGAQGKT ELLWFGQAGF RIKTPQGKMI LIDPWITGGP KTPPMYKNDL AAIGPIDLLL VTHAHVDHLG DAPTIAKTNN TKLYGPADMV TPLTTLGVLP AELGYRFNKT GQVTPLPGIK VTAVQAEHSS LLVWKNPATD KLESHPAGEP MGYIIELENG FKIWHMGDTG LFSDMKFISE HYKPDLVLIP IGGNFTMAPD DAAYALRTWV KPKMVIPMHY NSNPMTKGTL AEFQAAMKGS NIKVIPMTEG ETVQF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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