Recombinant Prosthecochloris vibrioformis Chaperone protein DnaJ(dnaJ)

Code CSB-YP392611PZJ
Size Pls inquire other sizes
Source Yeast
Code CSB-EP392611PZJ
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Source E.coli
Code CSB-EP392611PZJ-B
Size Pls inquire other sizes
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Code CSB-BP392611PZJ
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Source Baculovirus
Code CSB-MP392611PZJ
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Source Mammalian cell

Product Details

Purity >85% (SDS-PAGE)
Target Names dnaJ
Uniprot No. A4SFR5
Species Chlorobium phaeovibrioides (strain DSM 265 / 1930) (Prosthecochloris vibrioformis (strain DSM 265))
Expression Region 1-396
Target Protein Sequence MKKDYYEILG VGRSADKDEI KKAYRKLALK YHPDKNPDNK DAEDHFKEVN EAYEVLSNDD KRRRYDQFGH AGVGSSAASG GGAGAYGAGG ADFSDIFSAF NDMFGGGGGG RGRGGFEDAF GGGQRRSRAS AGIQGTDLKI RLKLSLEEIA KGVEKTLKIK KQVPCKECNG SGSKTGATET CPTCHGTGEV RQASKTMFGQ FVNIAACPTC GGEGRIVKDR CPACYGEGIK QGEVTVKVTV PAGVQDNNYL TLRGQGNSGP RGGASGDLIV VIEEKPHEFF VRNGDDVIYS LAVSFPDLVL GTKVEVPTLD GAIKMTIPPS TQPETMLRIP GHGIGHLRGS GKGDQYVRVN VFVPKDLSAT DKELLKGLRK SPGISPEAHG TGTEKSFFEK MKDIIG
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Lyophilized Form Lyophilized powder
Buffer before Lyophilization Lyophilized from Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.
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Target Data

Function Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins.
Subcellular Location Cytoplasm
Protein Families DnaJ family
Database Links

KEGG: pvi:Cvib_1312

STRING: 290318.Cvib_1312

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