Recombinant Protein Wnt-1 (cwn-1)

Code CSB-YP339509CXY
MSDS
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Source Yeast
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Code CSB-EP339509CXY
MSDS
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Source E.coli
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Code CSB-EP339509CXY-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP339509CXY
MSDS
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Source Baculovirus
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Code CSB-MP339509CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
cwn-1
Uniprot No.
Alternative Names
cwn-1; wnt-1; K10B4.6; Protein Wnt-1
Species
Caenorhabditis elegans
Expression Region
30-372
Target Protein Sequence
A NRFDRDKPGT SCKSLKGLTR RQMRFCKKNI DLMESVRSGS LAAHAECQFQ FHKRRWNCTL IDPVTHEVIP DVFLYENTRE SAFVHAISSA AVAYKVTRDC ARGISERCGC DYSKNDHSGK SQFQYQGCSD NVKFGIGVSK EFVDSAQRRV LMMKDDNGTS LLGPSQLSAD GMHMINLHNN QAGRQVLEKS LRRECKCHGM SGSCEMRTCW DSLPNFRHIG MAIKDKFDGA AEVKVVKEDG IEKPRIVMKN SQFKRHTNAD LVYMTPSPDF CESDPLRGIL GTKGRQCTLA PNAIDDCSLL CCGRGYEKKV QIVEEKCNCK FIYCCEVRCE PCQKRIEKYL CL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Binds receptor tyrosine kinase cam-1. Together with Wnt ligand cwn-2, regulates the migration of CAN, ALM, BDU and HSN neurons during embryogenesis, the migration of QL and QR neuroblast descendants during larval development, and polarity of ALM neurons. Also acts with the Wnt ligand egl-20 to direct HSN neuron migration. Acts through the Wnt receptor cfz-2 to direct ALM migration. Also plays a role in axon growth and guidance in HSN and male CP neurons. In addition, together with Wnt ligand cwn-2, negatively regulates developmental neurite pruning of AIM neurons probably by acting as a ligand for receptor tyrosine kinase cam-1. Probably by activating the Wnt/Frizzled pathway, may regulate vulva development. May act redundantly with other Wnt ligands such as cwn-2 and mom-2 to control seam cell polarity.
Gene References into Functions
  1. Here, too, we found that four of five Wnt receptors can influence P7.p orientation, suggesting that a surprising amount of functional redundancy exists in Wnt signaling during C. elegans vulval induction. PMID: 16930586
  2. CAM-1 interacts with and inhibits Egl-20, CWN-1 and MOM-2 during vulva morphogenesis. PMID: 17942487
  3. C. elegans Wnt homolog, cwn-1, functions to regulate asymmetric division of the ABpl/rpppa blast cell. Cwn-1 does not act alone in this process, and it functions with another redundant ligand that appears not to be a Wnt. PMID: 19389360
Subcellular Location
Secreted, extracellular space, extracellular matrix. Cytoplasm. Cell membrane; Peripheral membrane protein.
Protein Families
Wnt family
Tissue Specificity
Expressed in intestine, some head neurons and ventral nerve cord and pharyngeal neurons. Expressed in the tail and weakly expressed in the vulva and body wall muscles. Expressed highly in posterior dorsal and ventral muscle cells.
Database Links

KEGG: cel:CELE_K10B4.6

STRING: 6239.K10B4.6a

UniGene: Cel.18277

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