Recombinant Pseudomonas aeruginosa Acyl-homoserine lactone acylase quiP (quiP), partial

Code CSB-YP862280EZX
MSDS
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Source Yeast
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Code CSB-EP862280EZX
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Source E.coli
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Code CSB-EP862280EZX-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP862280EZX
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Source Baculovirus
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Code CSB-MP862280EZX
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
quiP
Uniprot No.
Alternative Names
quiP; PA1032; Acyl-homoserine lactone acylase QuiP; AHL acylase QuiP; Acyl-HSL acylase QuiP; EC 3.5.1.97; Quorum signal utilization and inactivation protein) [Cleaved into: Acyl-homoserine lactone acylase QuiP subunit alpha; Acyl-HSL acylase QuiP subunit alpha); Acyl-homoserine lactone acylase QuiP subunit beta; Acyl-HSL acylase QuiP subunit beta)]
Species
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the deacylation of acyl-homoserine lactone (AHL or acyl-HSL), releasing homoserine lactone (HSL) and the corresponding fatty acid. Possesses a specificity for the degradation of long-chain acyl-HSLs (side chains of seven or more carbons in length). Appears to be the acyl-HSL acylase that underlies the ability of P.aeruginosa to degrade and utilize certain acyl-HSLs as growth nutrients, including one of its own quorum signals, 3-oxo-C12-HSL. Is thought to have a role in quorum quenching.
Gene References into Functions
  1. identification of QuiP, the product of gene PA1032, as a second acyl-homoserine lactone acylase PMID: 16461666
Subcellular Location
Periplasm.
Protein Families
Peptidase S45 family
Database Links

KEGG: pae:PA1032

STRING: 208964.PA1032

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