Recombinant Pseudomonas aeruginosa HTH-type transcriptional regulator nfxB (nfxB)

Code CSB-YP341300EZX
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Source Yeast
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Code CSB-EP341300EZX
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Source E.coli
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Code CSB-EP341300EZX-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP341300EZX
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Source Baculovirus
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Code CSB-MP341300EZX
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
nfxB
Uniprot No.
Alternative Names
nfxB; PA4600; HTH-type transcriptional regulator NfxB
Species
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Expression Region
1-187
Target Protein Sequence
MTLISHDERL IKALAVAIVD RPRATLKELA EAAGVSKATL HRFCGTRDNL VQMLEDHGET VLNQIIQACD LEHAEPLEAL QRLIKEHLTH RELLVFLVFQ YRPDFLDPHG EGARWQSYLE ALDAFFLRGQ QKGVFRIDIT AAVFTELFIT LVYGMVDAER RGRAASSNSA HTLEQMFLHG ASNPARS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Confers resistance to guinolones. May negatively regulate the expression of genes that are associated with cell permeability to drugs.
Gene References into Functions
  1. A rapid development of resistance to subinhibitory concentrations of ciprofloxacin due to nfxB mutations was observed by live confocal laser scanning microscopy imaging of the flow cell biofilms. PMID: 27993856
  2. These results suggest that NfxB operates as a tetramer (dimer of dimers) and that the C terminus of the protein serves as a tetramerization domain. PMID: 23924707
  3. Data suggest that many of the nfxB mutant phenotypes are not caused by the direct effects of the NfxB regulator, but instead by inappropriate mexCD-oprJ expression. PMID: 20373734
Database Links

KEGG: pae:PA4600

STRING: 208964.PA4600

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