Recombinant Pseudomonas aeruginosa Outer membrane protein oprM (oprM)

Code CSB-YP690397EZX
MSDS
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Source Yeast
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Code CSB-EP690397EZX
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Source E.coli
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Code CSB-EP690397EZX-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP690397EZX
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Source Baculovirus
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Code CSB-MP690397EZX
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
oprM
Uniprot No.
Alternative Names
oprM; oprK; PA0427; Outer membrane protein OprM
Species
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Expression Region
18-485
Target Protein Sequence
CSL IPDYQRPEAP VAAAYPQGQA YGQNTGAAAV PAADIGWREF FRDPQLQQLI GVALENNRDL RVAALNVEAF RAQYRIQRAD LFPRIGVDGS GTRQRLPGDL STTGSPAISS QYGVTLGTTA WELDLFGRLR SLRDQALEQY LATEQAQRSA QTTLVASVAT AYLTLKADQA QLQLTKDTLG TYQKSFDLTQ RSYDVGVASA LDLRQAQTAV EGARATLAQY TRLVAQDQNA LVLLLGSGIP ANLPQGLGLD QTLLTEVPAG LPSDLLQRRP DILEAEHQLM AANASIGAAR AAFFPSISLT ANAGTMSRQL SGLFDAGSGS WLFQPSINLP IFTAGSLRAS LDYAKIQKDI NVAQYEKAIQ TAFQEVADGL AARGTFTEQL QAQRDLVKAS DEYYQLADKR YRTGVDNYLT LLDAQRSLFT AQQQLITDRL NQLTSEVNLY KALGGGWNQQ TVTQQQTAKK EDPQA
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
The outer membrane component of the MexAB-OprM efflux system that confers multidrug resistance. Also functions as the major efflux pump for n-hexane and p-xylene efflux. Over-expression of the pump increases antibiotic and solvent efflux capacities. Can replace the OprJ outer membrane component of the MexCD-OprJ pump; the antibiotics exported are those exported by the intact MexCD pump, showing that efflux substrate specificity is not conferred by this component. Serves as the outer membrane component for the MexXY efflux system. Implicated in the secretion of the siderophore pyoverdine. OprM is probably involved in the efflux of the siderophore across the outer membrane.; The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the MexB subunit is a proton antiporter.
Gene References into Functions
  1. Polymyxin susceptibility in Pseudomonas aeruginosa is linked to the MexXY-OprM multidrug efflux system. PMID: 26369970
  2. MexR termination has a role in overexpression of MexAB-OprM efflux pump in Pseudomonas aeruginosa PMID: 26866484
  3. The mechanism of development from sensitivity to resistance of P. aeruginosa to carbapenem during carbapenem treatment is due to porin oprD2 loss and an increased expression level of MexXY-OprM. PMID: 26125881
  4. Efflux pump MexAB-OprM plays an important role in antibiotic resistance in P. aeruginosa, because of acting the efflux pumps on antibiotics in a non-specific manner PMID: 25644798
  5. The results demonstrate a new quorum sensing regulation mechanism via the efflux system MexAB-OprM in Pseudomonas aeruginosa. PMID: 22574700
  6. Pentachlorophenol induction of the Pseudomonas aeruginosa mexAB-oprM efflux operon involves repressors NalC and MexR and the antirepressor ArmR PMID: 22393435
  7. Opening of the OprM channel is accompanied by the simultaneous exposure of a protein structure resembling a six-bladed cogwheel, which intermeshes with the complementary cogwheel structure in the MexA hexamer. PMID: 22308040
  8. Chlorinated phenols control the expression of the MexA, MexB and OprM by interacting with NalC. PMID: 21231970
  9. Data show that the protein layers were composed of MexA-OprM complexes self-assembled into regular arrays into proteoliposomes. PMID: 20599691
  10. MexB acts independently of its cognitive partners, MexA and OprM. PMID: 20583998
  11. analysis of the opening mechanism of P. aeruginosa OprM channel PMID: 20399187
  12. Data show that loss of PA3719 but not of PA3720 expression in a spontaneous nalC mutant reduced MexAB-OprM expression to wild-type levels and compromised multidrug resistance. PMID: 15387820
  13. crystal structure solution of OprM offers possible mechanisms of pore opening and pump assembly PMID: 15507433
  14. These data strongly suggest that when derepressed, MexAB-OprM provides P. aeruginosa with a resistance that may be sufficient to impair the efficacy of single therapy with highly potent fluoroquinolones. PMID: 15722391
  15. Two-dimensional (2D) crystallization of OprM into lipid bilayers and the determination of its 2D projected structure by cryo-electron crystallography. PMID: 15797729
  16. Crystal diffracted to 2.56 A resolution using 0.9 A X-rays from the synchrotron-radiation source. A heavy-atom derivative for isomorphous replacement phasing was obtained using iridium chloride. PMID: 16508113
  17. OprM and OprN belong to the outer membrane factor family proteins which have been expressed in Escherichia coli and purified. PMID: 16511029
  18. These data highlight the importance of MexA and interactions with this adapter in promoting MexAB-OprM pump assembly and in stabilizing the pump complex. PMID: 17586626
  19. Using a series of efflux mutants of Pseudomonas aeruginosa, the MexAB-OprM pump was identified as contributing to this organism's tolerance to the antimicrobial agent tea tree (Melaleuca alternifolia) oil and its monoterpene components. PMID: 18192403
  20. Andrographolide recovered P. aeruginosa susceptibility to antibiotics and reduced the MexAB-OprM efflux pump expression level. PMID: 18304418
  21. Data demonstrate that the subpopulation of metabolically active cells is able to adapt to colistin by inducing an unspecific adaptation mechanism mediated by the mexAB-oprM genes. PMID: 18312276

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Subcellular Location
Cell outer membrane; Lipid-anchor.
Protein Families
Outer membrane factor (OMF) (TC 1.B.17) family
Database Links

KEGG: pae:PA0427

STRING: 208964.PA0427

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