Recombinant Rat Anionic trypsin-2 (Prss2)

Code CSB-EP018814RA
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Source E.coli
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Code CSB-EP018814RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018814RA
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Source Baculovirus
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Code CSB-MP018814RA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Prss2
Uniprot No.
Alternative Names
Prss2; Try2; Anionic trypsin-2; EC 3.4.21.4; Anionic trypsin II; Pretrypsinogen II; Serine protease 2
Species
Rattus norvegicus (Rat)
Expression Region
-
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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 Q&A
Q:

I have a question regarding Recombinant Rat Anionic trypsin-2(Prss2) # CSB-EP018814RA, CSB-YP018814RA that we purchased for research use.
Could you provide us the amino acid sequence of this product?
Is this protein active or proform?

A:
Very nice to receive your inquiry. Recombinant Rat Anionic trypsin-2(Prss2) # CSB-EP018814RA, CSB-YP018814RA
Pls check the product information as follows:
Expression Region: 24-246aa; Full length of the mature protein.
Tag information: EP, BP, MP: N-terminal 10xHis-tagged and C-terminal Myc-tagged; YP: N-terminal 6xHis-tagged
Target protein Sequence:(Not include the tag sequence)

IVGGYTCQENSVPYQVSLNSGYHFCGGSLINDQWVVSAAHCYKSRIQVRLGEHNINVLEGNEQFVNAAKIIKHPNFDRKTLNNDIMLIKLSSPVKLNARVATVALPSSCAPAGTQCLISGWGNTLSSGVNEPDLLQCLDAPLLPQADCEASYPGKITDNMVCVGFLEGGKDSCQGDSGGPVVCNGELQGIVSWGYGCALPDNPGVYTKVCNYVDWIQDTIAAN


Regarding the activity, sorry, we haven't tested the activity of these proteins for the time being, however, as what we provide is the full length of the mature protein,
and they are purified under very mild environment, in theory, it should be active. We recommend you to purchase a small size to have a try.
If you don't know how to choose the most suitable expression system to express the protein, Pls refer to this information "Which expression system suits your experiment most ?" on our website.
https://www.cusabio.com/protein_service/
Or you can tell us your application, we can analyze for you and recommend the most suitable product to you.

Target Background

Gene References into Functions
  1. activated microglia increase neurogenesis through secretion of PRSS2. PMID: 23650361
  2. Nicotine impairs secretion rather than production of pancreatic trypsinogen 2. PMID: 18815551
Subcellular Location
Secreted, extracellular space.
Protein Families
Peptidase S1 family
Database Links
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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