Product Details

Purity

>85% (SDS-PAGE)

Target Names

Camk2d

Uniprot No.

P15791

Alternative Names

Camk2dCalcium/calmodulin-dependent protein kinase type II subunit delta; CaM kinase II subunit delta; CaMK-II subunit delta; EC 2.7.11.17

Species

Rattus norvegicus (Rat)

Expression Region

2-533

Target Protein Sequence

ASTTTCTRF TDEYQLFEEL GKGAFSVVRR CMKIPTGQEY AAKIINTKKL SARDHQKLER EARICRLLKH PNIVRLHDSI SEEGFHYLVF DLVTGGELFE DIVAREYYSE ADASHCIQQI LESVNHCHLN GIVHRDLKPE NLLLASKSKG AAVKLADFGL AIEVQGDQQA WFGFAGTPGY LSPEVLRKDP YGKPVDMWAC GVILYILLVG YPPFWDEDQH RLYQQIKAGA YDFPSPEWDT VTPEAKDLIN KMLTINPAKR ITASEALKHP WICQRSTVAS MMHRQETVDC LKKFNARRKL KGAILTTMLA TRNFSAAKSL LKKPDGVKIN NKANVVTSPK ENIPTPALEP QTTVIHNPDG NKESTESSNT TIEDEDVKAR KQEIIKVTEQ LIEAINNGDF EAYTKICDPG LTAFEPEALG NLVEGMDFHR FYFENALPKI NKPIHTIILN PHVHLVGDDA ACIAYIRLTQ YMDGNGMPKT MQSEETRVWH RRDGKWQNIH FHRSGSPTVP IKPPCIPNGK ENFSGGTSLW QNI

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca(2+) homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca(2+) influx into the myocyte, Ca(2+) release from the sarcoplasmic reticulum (SR), SR Ca(2+) uptake and Na(+) and K(+) channel transport. Targets also transcription factors and signaling molecules to regulate heart function. In its activated form, is involved in the pathogenesis of dilated cardiomyopathy and heart failure. Contributes to cardiac decompensation and heart failure by regulating SR Ca(2+) release via direct phosphorylation of RYR2 Ca(2+) channel on 'Ser-2808'. In the nucleus, phosphorylates the MEF2 repressor HDAC4, promoting its nuclear export and binding to 14-3-3 protein, and expression of MEF2 and genes involved in the hypertrophic program. Is essential for left ventricular remodeling responses to myocardial infarction. In pathological myocardial remodeling acts downstream of the beta adrenergic receptor signaling cascade to regulate key proteins involved in ECC. Regulates Ca(2+) influx to myocytes by binding and phosphorylating the L-type Ca(2+) channel subunit beta-2 CACNB2. In addition to Ca(2+) channels, can target and regulate the cardiac sarcolemmal Na(+) channel Nav1.5/SCN5A and the K+ channel Kv4.3/KCND3, which contribute to arrhythmogenesis in heart failure. Phosphorylates phospholamban (PLN/PLB), an endogenous inhibitor of SERCA2A/ATP2A2, contributing to the enhancement of SR Ca(2+) uptake that may be important in frequency-dependent acceleration of relaxation (FDAR) and maintenance of contractile function during acidosis. May participate in the modulation of skeletal muscle function in response to exercise, by regulating SR Ca(2+) transport through phosphorylation of PLN/PLB and triadin, a ryanodine receptor-coupling factor.

Gene References into Functions

In vitro, overexpression of miR-30c or miR-30e inhibited CaMKIIdelta2 protein expression by ~50% in cultured rat aortic vascular smooth muscle cells, and inhibited vascular smooth muscle cell proliferation and migration. PMID: 27199283
up-regulation of CaMKIIdelta may contribute to the phenylephrine-induced myocardial hypertrophy through the activation of store-operated Ca2+ entry expressed in the cardiomyocytes PMID: 27940402
Enhanced activation of Dyrk1A-ASF-CaMKIIdelta signaling pathway may underlie the mechanisms of HF after MI, and Dyrk1A inhibition may contribute to inactivation of this pathway and thereby retard the progression of MI-induced HF. PMID: 26067684
CaMKIIdelta splice variants are selectively susceptible to autophosphorylation and this is associated with arrhythmia suppression in the female heart. PMID: 25539453
CaMKII inhibition may mitigate cardiac contractile dysfunction, at least partially, by limiting the contents of not only apoptotic, but also necroptotic proteins. PMID: 24347176
The expression of CaMKII delta, but not beta or gamma, is reduced in septic muscle. PMID: 23238742
Thrombin activated a PLC/Ca(2+)/CaMKII/PKCalpha/p42/p44 MAPK and JNK1/2 pathway, which in turn triggered AP-1 activation and ultimately induced MMP-9 expression. PMID: 23585120
The expression of miR-30b-5p is down-regulated in cardiac hypertrophy, and restoration of its function inhibits the expression of CaMKIIdelta. PMID: 23360843
negative regulation of CREB activity by endogenous CaMKIIdelta-dependent CREB-Ser(142) phosphorylation and suggest a potential mechanism for CaMKIIdelta/CREB signaling in modulating proliferation and migration in VSM cells. PMID: 24106266
Data indicate a mechanism by which CaMKIIdelta2 and Fyn may coordinately regulate vascular smooth muscle cell motility. PMID: 24003228
Phosphorylation at specific PEVK/titin N2B-unique sequence sites was amplified in transgenic mice bearing rat CAMK2d versus WT hearts. PMID: 23283722
CaMKIIdelta may be implicated in the apoptosis of neurons and the recovery of neurological outcomes after brain injury. PMID: 22048920
It was concluded that cytoskeletal effects of Cd in mesangial cells are partially mediated by Cd-dependent activation of CaMK-IIdelta, binding of CaMK-IIdelta and gelsolin to actin filaments, and cleavage of gelsolin. PMID: 22553113
CaMKIIdelta2 mediates MEF2-dependent gene transcription in VSMCs through regulation of HDAC4 and HDAC5. PMID: 22360269
These results revealed the important role of CaMKIIdelta in Ca(2+) disorder caused by electrical field stimulation. PMID: 21575598
cardiac hypertrophy pathological changes were largely blunted by silencing the CaMKIIdeltaA gene and an underlying mechanism indicated selective interference with the HDAC4-MEF2 signaling pathway. PMID: 21554860
Polymorphisms in calcium/calmodulin dependent protein kinase II delta (Camk2d) are associated with seizure susceptibility in Sprague-Dawley rat models. PMID: 20638246
oxidation-dependent facilitation of L-type Ca(2+) channels is mediated by oxidation-dependent CaMKII activation, in which local Ca(2+) increases induced by SR Ca(2+) release is required PMID: 19883656
Calcium/Calmodulin-dependent protein kinase II delta 6 (CaMKIIdelta6) and RhoA is involved in thrombin-induced endothelial barrier dysfunction PMID: 20442409
interaction of RAGE and its ligand S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53 signaling. PMID: 19910575
Calcium/calmodulin-dependent protein kinase IIdelta 2 and gamma isoforms regulate potassium currents of rat brain capillary endothelial cells under hypoxic conditions PMID: 11925434
mechanism of CaM kinase IIdeltaC silencing of MEF-2-dependent genes is by phosphorylation of 14-3-3beta, which allows HDAC to return to the nucleus to reform a complex with MEF2, thereby silencing MADS box-dependent gene induction in smooth muscle PMID: 12619878
Expression of the constitutively active CaMKII delta(2) mutant inhibited PDGF-stimulated cell migration. PMID: 14761894
These results suggest that increased phosphorylation of CREB protein may contribute to central sensitization following acute peripheral noxious stimuli, and the effect may be regulated through the activation of CaM kinase cascades. PMID: 15631885
CaMKIIdelta(C) negatively regulates RyR2 activity and spontaneous sarcoplasmic reticulum Ca(2+) release, thereby affording a negative feedback that stabilizes local and global Ca(2+)-induced Ca(2+) release in the heart. PMID: 17234969
The role fo CaMKIIdelta in the regualtion of vascular smooth muscle cell proliferation is reported. PMID: 17267544
CaMKIIdelta(2) may be an important regulator of inducible nitric oxide synthase trafficking and activity in vascular smooth muscle cells. PMID: 17293490
CaMKIIdelta2 is specifically induced in VSM and adventitial fibroblasts during the response of an artery to injury and is a positive regulator of proliferation and migration in the vessel wall contributing to neointima formation and vascular remodeling. PMID: 18096823
CaM kinase II delta2-dependent regulation of vascular smooth muscle cell polarization and migration PMID: 18385282
Results suggest that the inhibitory effect of kappa-opioid receptor stimulation on beta(1)-adrenoceptor stimulation may involve CaMKIIdelta. PMID: 18721804
Oestrogen confers cardioprotection at least partly by suppressing CaMKIIdelta. PMID: 19422373
CaMKIIdeltaB plays an essential role in cardiomyocyte survival PMID: 19602725

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Subcellular Location

Cell membrane, sarcolemma; Peripheral membrane protein; Cytoplasmic side. Sarcoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.; [Isoform Delta 1]: Nucleus.

Protein Families

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, CaMK subfamily

Tissue Specificity

Isoform Delta 1 is the predominant form in the brain, isoform Delta 2 and isoform Delta 3 predominate in the aorta and isoform Delta 4 in skeletal muscle.

Database Links

KEGG: rno:24246

STRING: 10116.ENSRNOP00000016026

UniGene: Rn.87208

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Recombinant Rat Calcium/calmodulin-dependent protein kinase type II subunit delta (Camk2d)

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