Recombinant Rat Class A basic helix-loop-helix protein 15 (Bhlha15)

Code CSB-YP002684RA
MSDS
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Source Yeast
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Code CSB-EP002684RA
MSDS
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Source E.coli
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Code CSB-EP002684RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002684RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP002684RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Bhlha15
Uniprot No.
Alternative Names
Bhlha15; Bhlhb8; Mist1Class A basic helix-loop-helix protein 15; bHLHa15; Class B basic helix-loop-helix protein 8; bHLHb8; Muscle; intestine and stomach expression 1; MIST-1
Species
Rattus norvegicus (Rat)
Expression Region
1-197
Target Protein Sequence
MKTKNRPPRR RTPMQDAEAT PGEQTPDRSQ SGSGASEVTK GLRSRTARAS GTRAEVSRRR QGSSSRRENS VQRRLESNER ERQRMHKLNN AFQALREVIP HVRADKKLSK IETLTLAKNY IKSLTATILT MSSSRLPGLE APGPAPGPKL YQHYHHQQQQ QQQQQQVAGA VLGVTEDQPQ GHLQRYSTQI HSFREGS
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a role in controlling the transcriptional activity of MyoD, ensuring that expanding myoblast populations remain undifferentiated. Repression may occur through muscle-specific E-box occupancy by homodimers. May also negatively regulate bHLH-mediated transcription through an N-terminal repressor domain. Serves as a key regulator of acinar cell function, stability, and identity. Also required for normal organelle localization in exocrine cells and for mitochondrial calcium ion transport. May function as a unique regulator of gene expression in several different embryonic and postnatal cell lineages. Binds to the E-box consensus sequence 5'-CANNTG-3'.
Gene References into Functions
  1. bHLH regulate ABP in differentiated Sertoli cells. PMID: 15112319
  2. Data suggest that Mist1 activates exocrine-specific gene transcription through an acetylation mechanism. PMID: 17605298
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in liver, spleen and olfactory epithelium. Weaker expression is seen in skeletal muscle, cardiac muscle, eye and brain tissue.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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