Recombinant Rat DNA-binding protein inhibitor ID-2 (Id2)

Code CSB-YP010967RA
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Source Yeast
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Code CSB-EP010967RA
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Source E.coli
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Code CSB-EP010967RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP010967RA
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Source Baculovirus
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Code CSB-MP010967RA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Id2
Uniprot No.
Alternative Names
Id2; Id-2; DNA-binding protein inhibitor ID-2; Inhibitor of DNA binding 2; Inhibitor of differentiation 2
Species
Rattus norvegicus (Rat)
Expression Region
1-134
Target Protein Sequence
MKAFSPVRSV RKNSLSDHSL GISRSKTPVD DPMSLLYNMN DCYSKLKELV PSIPQNKKVT KMEILQHVID YILDLQIALD SHPTIVSLHH QRPGQNQTSR TPLTTLNTDI SILSLQASEF PSELMSNDSK VLCG
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional regulator (lacking a basic DNA binding domain) which negatively regulates the basic helix-loop-helix (bHLH) transcription factors by forming heterodimers and inhibiting their DNA binding and transcriptional activity. Implicated in regulating a variety of cellular processes, including cellular growth, senescence, differentiation, apoptosis, angiogenesis, and neoplastic transformation. Inhibits skeletal muscle and cardiac myocyte differentiation. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer. Restricts the CLOCK and ARNTL/BMAL1 localization to the cytoplasm. Plays a role in both the input and output pathways of the circadian clock: in the input component, is involved in modulating the magnitude of photic entrainment and in the output component, contributes to the regulation of a variety of liver clock-controlled genes involved in lipid metabolism.
Gene References into Functions
  1. Here, the authors discovered that, in the developing neuron from rat, Akt signaling regulates axon growth and growth cone formation through phosphorylation of serine 14 (S14) on Inhibitor of DNA binding 2 (Id2). PMID: 27938661
  2. Knockdown of Id2 alleviates neuropathic pain by inhibiting the NF-kappaB activation. PMID: 26768262
  3. The data support the implication of inhibitor of DNA binding/differentiation 2 in the modulation of hypoxia/ischemia-induced neuronal apoptosis. PMID: 26187693
  4. Hypoxia/ischemia (H/I) up-regulates Id2 expression in neuronal cells, and Id2 might play an important role in H/I-induced neuronal apoptosis. PMID: 23994058
  5. NF-kappaB p65 can facilitate early fracture healing of rat radius by elevating PGE2 production and regulating BMP-7 and Id2 expression. PMID: 21675116
  6. CpG-rich islands within the Id2 and Id4 genes were bound by PRMT5 and were hypomethylated in PRMT5-deficient cells, suggesting that PRMT5 plays a role in gene silencing during glial cell PMID: 22041901
  7. A novel transcriptional-dependent mechanism of Id2 regulation by GSH and oxidative stress induced by acetaminophen-overdose through the indirect modulation of the proteasome pathway. PMID: 20815767
  8. Id2 protein is integrated into the cell cycle regulatory cascade that results in SMC proliferation following vascular injury and suggest that this effect is at least in part via a cdk2-dependent inhibition of p21 gene expression. PMID: 11706002
  9. induction of Id2 and resultant inhibition of basic helix-loop-helix transcription factor-regulated genes in Sertoli cells may contribute to the regulation of spermatogenesis PMID: 14761970
  10. Id2 & p53 were elevated in cytosolic fraction of suspended muscle in young & aged animals but not in the nuclear protein fraction; findings are consistent with the hypothesis that Id2 & p53 are responsive to unloading-induced muscle atrophy PMID: 16282427
  11. The results indicated that Id2 mRNA is expression in these regions include the amygdaloid complex, caudate putamen, globus pallidus, substantia nigra pars reticulata, suprachiasmatic nucleus, and the anterior part of the subventricular zone. PMID: 16443197
  12. Hypoxia did inhibit the downregulation of the trophoblast stem cell marker Id2. PMID: 16481593
  13. A control role for Id2 in hepatocyte priming, through a p130 dissociation-independent regulation of c-myc, in regenerating rat liver is reported. PMID: 16776654
  14. These observations suggest that Id2 may be mainly involved in terminal maturation of oligodendrocytes and myelination. PMID: 18029094
  15. TSH regulates Id2 expression, but that Id2 does not alter the expression of thyroid-specific genes, such as Tg, in FRTL-5 thyroid cells PMID: 18437010

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Subcellular Location
Cytoplasm. Nucleus.
Database Links
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