Recombinant Rat Ectonucleoside triphosphate diphosphohydrolase 2 (Entpd2), partial

Code CSB-YP007691RA1
MSDS
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Source Yeast
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Code CSB-EP007691RA1
MSDS
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Source E.coli
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Code CSB-EP007691RA1-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007691RA1
MSDS
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Source Baculovirus
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Code CSB-MP007691RA1
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Entpd2
Uniprot No.
Alternative Names
Entpd2; Cd39l1; Ectonucleoside triphosphate diphosphohydrolase 2; NTPDase 2; CD39 antigen-like 1
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Hydrolyzes ADP only to a marginal extent.
Gene References into Functions
  1. Apo and complex structures of the rat NTPDase2 extracellular domain and Legionella pneumophila NTPDase1. PMID: 23830739
  2. two ENTPD isoforms, ENTPD-1 and -2, are expressed in adult rat ventricular CFs PMID: 23677997
  3. NTPDase2 overexpression modulates pro-inflammatory cytokine production and platelet reactivity. PMID: 22038661
  4. ATP acts in an autocrine manner to promote its own release and that of dopamine, an action that is controlled by the level of E-NTPDase1/CD39 expression PMID: 21325440
  5. NTPDase2 was expressed in the periportal region surrounding intrahepatic bile ducts PMID: 12395323
  6. novel inducible release of full length particulate CD39, and propose its role in the physiological context of pancreatic secretion. PMID: 12832497
  7. NTPDase2 is associated with nonmyelinating Schwann cells of the rat sciatic nerve. It was associated with the satellite glial cells in dorsal root ganglia and sympathetic ganglia, and with the enteric ganglion glia. PMID: 14730706
  8. hepatic stellate cells differentially regulate nucleoside triphosphate diphosphohydrolase expression after activation PMID: 14764443
  9. the amplitude of the long-lasting phase of the Ca(2+) signal depended on the balance between agonist degradation by ectonucleotidases and agonist transport, by diffusion and convection, from bulk solution to the EC surface PMID: 15004436
  10. alternative coding of the intracellular C-terminal domain contributes distinctive phenotypic variation with respect to extracellular nucleotide specificity, hydrolysis kinetics, protein kinase-dependent intracellular regulation and protein trafficking PMID: 15362980
  11. down-regulation in biliary cirrhosis PMID: 15651265
  12. NTPDase2 has a role in portal fibroblast regulation of bile duct epithelium proliferation PMID: 15799977
  13. NTPDase2 may modulate inflammatory reactions within the central nervous system and could represent a useful therapeutic target in human disease. PMID: 16414200
  14. NTPdase2 expression profile was studied in the Walker 256 carcinoma. PMID: 17574764
  15. NTPDase2 ectodomain refolded from bacterial inclusion bodies was characterized. PMID: 17910474
  16. Data showed that Entpd2 as the enzyme with the highest expression in left ventricle. PMID: 18201730
  17. IL-6 transcriptionally downregulates NTPDase2 expression by portal fibroblasts via actions at specific promoter elements independently of myofibroblastic differentiation PMID: 18202114
  18. The structure of the extracellular domain of Rattus norvegicus NTPDase2 in an active state at resolutions between 1.7 A and 2.1 A in four different forms, is presented. PMID: 18458329
  19. Selective NTPDase2 expression modulates in vivo rat glioma growth. PMID: 19558578

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Subcellular Location
[Isoform 1]: Cell membrane; Multi-pass membrane protein.
Protein Families
GDA1/CD39 NTPase family
Tissue Specificity
Expressed in brain, heart, vas deferens, kidney, skeletal muscle, thymus, lung and spleen. Weak expression in liver.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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