Recombinant Rat Growth factor receptor-bound protein 14 (Grb14)

Code CSB-YP009888RA
MSDS
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Source Yeast
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Code CSB-EP009888RA
MSDS
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Source E.coli
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Code CSB-EP009888RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009888RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP009888RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Grb14
Uniprot No.
Alternative Names
Grb14Growth factor receptor-bound protein 14; GRB14 adapter protein
Species
Rattus norvegicus (Rat)
Expression Region
2-538
Target Protein Sequence
TTSLQDGQS AAGRAGAQDS PLAVQVCRVA QGKGDAQDPA QVPGLHALSP ASDATRRGAM DRRKAKDLEV QETPSIPNPF PELCCSPLTS VLSAGLFPRS NSRKKQVIKV YSEDETSRAL EVPSDVTARD VCQLLILKNH YVDDNSWTLF EHLSHTGVER TVEDHELLTE VLSHWVMEED NKLYLRKNYA KYEFFKNPMY FFPEHMVSFA TEMNGDRSLT QIPQVFLSSN TYPEIHGFLH AKEQGKKSWK KAYFFLRRSG LYFSTKGTSK EPRHLQFFSE FSTSNVYMSL AGKKKHGAPT PYGFCFKPTK AGGPRDLKML CAEEDQSRMC WVTAIRLLKY GMQLYQNYMH PSQARSACSS QSVSPMRSVS ENSLVAMDFS GQKTRVIDNP TEALSVAVEE GLAWRKKGCL RLGNHGSPTA PSQSSAVNMA LHRSQPWFHH RISRDEAQQL ITRQGPVDGV FLVRDSQSNP RTFVLSMSHG QKIKHFQIIP VEDDGEVFHT LDDGHTKFTD LIQLVEFYQL NKGVLPCKLK HYCARMAV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Adapter protein which modulates coupling of cell surface receptor kinases with specific signaling pathways. Binds to, and suppresses signals from, the activated insulin receptor (INSR). Potent inhibitor of insulin-stimulated MAPK3 phosphorylation. Plays a critical role regulating PDPK1 membrane translocation in response to insulin stimulation and serves as an adapter protein to recruit PDPK1 to activated insulin receptor, thus promoting PKB/AKT1 phosphorylation and transduction of the insulin signal.
Gene References into Functions
  1. These results show that Chfr and Grb14 collaborate in a negative feedback loop controlling insulin-stimulated cell division. PMID: 25578860
  2. The phosphorylation status of the BPS region of Grb14 determines the positive or negative role it will play in insulin receptor signaling. PMID: 24350791
  3. Grb14 is a normal physiological modulator of CNG channel function in vivo. PMID: 20890309
  4. endogenous Grb14 exerts a negative feedback control on insulin receptor catalytic activity. This study thus demonstrates that Grb14 is a physiological regulator of liver insulin signaling PMID: 18657188
Subcellular Location
Cytoplasm. Endosome membrane; Peripheral membrane protein.
Protein Families
GRB7/10/14 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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