Product Details

Purity

>85% (SDS-PAGE)

Target Names

Kcnj2

Uniprot No.

Q64273

Alternative Names

Kcnj2; Irk1; Inward rectifier potassium channel 2; Inward rectifier K(+ channel Kir2.1; IRK-1; RBL-IRK1; Potassium channel, inwardly rectifying subfamily J member 2

Species

Rattus norvegicus (Rat)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

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Target Background

Function

Probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium.

Gene References into Functions

Report coupling of cardiac Kir2.1 and Kir2.2 subunits with the plasma membrane-bound AKAPs 15 and 79. Cardiac membrane-associated AKAPs are a functionally essential part of the regulatory cascade determining IK1 current function. PMID: 28331977
Moreover, the in vivo study showed that rat-VSMC switched to proliferative phenotypes and that knockdown of Kir2.1 significantly inhibited neointimal formation after rat carotid injury. Kir2.1 may be a potential therapeutic target in the treatment of cardiovascular diseases, such as atherosclerosis and restenosis following percutaneous coronary intervention. PMID: 27387235
Th1 response regulates Kir2.1 expression in infarcted hearts and valsartan regulates Th1 response to improve cardiac ion channel remodeling following myocardial infarction. PMID: 26566151
Kir2 channels composed of Kir2.1-2.3 subunits are expressed in neuronal and glial cells in the DRG and spinal cord, contributing to sensory transduction and motor control. PMID: 26854211
Immunohistochemical analysis reveals the expression of Kir2.1, Kir2.2, and Kir2.3 within spontaneously bursting neurons that regulate the level of pacemaker activity within the developing superficial dorsal horn. PMID: 23426663
The inward rectifier potassium current was clearly inhibited after the inhibition of KCNJ2 gene expression by RNA interference. PMID: 22426100
Vascular smooth muscle Kir2.1 channels are involved in the nitroprusside-induced dilation of rat-tail small arteries. PMID: 14993195
In PCR analyses using isolated juxtaglomerular cells, the mRNA for Kir2.1 (Kcnj2) and Kir2.2 (Kcnj12) was detected. Kir2.1 is the dominant component of the channel. PMID: 15284349
The expression of Kir4.1 mRNA was reduced by 55% after ischemia while the expression of Kir2.1 mRNA was not altered. PMID: 16330144
Immunochemical staining verified that both pericytes and endothelial cells of descending vasa recta express K(IR)2.1, K(IR)2.2, and K(IR)2.3 subunits. PMID: 17670900
In addition, LV-mediated overexpression of Kir2.1 opens up the prospect of studying the functional role of inward rectifier K(+) current in cardiac arrhythmias. PMID: 17675572
Biophysical properties of K(IR) (comprised K(IR)2.1 and 2.2 subunits) have a substantive role in enabling agonists to alter the electrical state of a multilayered artery. PMID: 18063660
Experiments demonstrated surface expression of Kir2.1-containing channels in astrocytes and in populations of pyramidal and nonpyramidal cells. Findings for astrocytes were not consistent with predictions for spatial buffering over substantial distance. PMID: 18076085

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Subcellular Location

Membrane; Multi-pass membrane protein. Membrane; Lipid-anchor.

Protein Families

Inward rectifier-type potassium channel (TC 1.A.2.1) family, KCNJ2 subfamily

Tissue Specificity

Prominently expressed in the central nervous system. Also found in other excitable tissues such as heart and skeletal muscle.

Database Links

KEGG: rno:29712

STRING: 10116.ENSRNOP00000006254

UniGene: Rn.44415

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Recombinant Rat Inward rectifier potassium channel 2 (Kcnj2), partial

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