Recombinant Rat Lymphoid enhancer-binding factor 1 (Lef1)

Code CSB-YP012856RA
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Source Yeast
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Code CSB-EP012856RA
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Source E.coli
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Code CSB-EP012856RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP012856RA
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Source Baculovirus
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Code CSB-MP012856RA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Lef1
Uniprot No.
Alternative Names
Lef1; Lymphoid enhancer-binding factor 1; LEF-1
Species
Rattus norvegicus (Rat)
Expression Region
1-397
Target Protein Sequence
MPQLSGGGGG GDPELCATDE MIPFKDEGDP QKEKIFAEIS HPEEEGDLAD IKSSLVNESE IIPASNGHEV VGQTQSSQEP YHDKAREHPD DGKHPDGGLY NKGPSYSSYS GYIMMPNMNS DPYMSNGSLS PPIPRTSNKV PVVQPSHAVH PLTPLITYSD EHFSPGSHPS HIPSEVNPKQ GMSRHPPAPE MPTFYPLSPG GVGQITPPLG WQGQPVYPIT GGFRQAYPSS LSGDTSMSRF SHHMIPGPPG PHTTGIPHPA IVTPQVKQEH PHTDSDLMHV KPEHEQRKEQ EPKRPHIKKP LNAFMLYMKE MRANVVAECT LKESAAINQI LGRRWHALSR EEQAKYYELA RKERQLHMQL YPGWSARDNY GKKKKRKREK LQESTSGTGP RMTAAYI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor that binds DNA in a sequence-specific manner. Participates in the Wnt signaling pathway. Activates transcription of target genes in the presence of CTNNB1 and EP300. PIASG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Regulates T-cell receptor alpha enhancer function. Required for IL17A expressing gamma-delta T-cell maturation and development, via binding to regulator loci of BLK to modulate expression. May play a role in hair cell differentiation and follicle morphogenesis.
Gene References into Functions
  1. our results demonstrate that Lef1 plays an important role in bulge stem cell differentiation, promoting beta-catenin translocation into the nucleus, activating downstream signaling molecules PMID: 23630438
  2. investigation of biological roles of Lef1 in regulating odontoblast differentiation: overexpression/knockdown of Lef1 appears to control expression of dentin sialoprotein, osteocalcin, and alkaline phosphatase PMID: 20425127
  3. TGF-beta signaling enhances TCF-4 and lymphoid enhancing factor-1 mRNA expression and increases TCF-4 transcriptional activity. PMID: 20093419
  4. Wnt-1 induction of cytosolic beta-catenin and Tcf/Lef transcriptional activation correlate with enhanced proliferation, survival, and post-confluent growth. PMID: 14641328
Subcellular Location
Nucleus.
Protein Families
TCF/LEF family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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