Recombinant Rat Mitogen-activated protein kinase 3(Mapk3)

Code CSB-YP013456RA
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Source Yeast
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Code CSB-EP013456RA
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Source E.coli
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Code CSB-EP013456RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP013456RA
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Source Baculovirus
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Code CSB-MP013456RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Mapk3
Uniprot No. P21708
Alternative Names Mapk3; Erk1; Prkm3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; EC 2.7.11.24; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; MNK1; Microtubule-associated protein 2 kinase; p44-ERK1
Species Rattus norvegicus (Rat)
Expression Region 2-380
Target Protein Sequence AAAAAAPGG GGGEPRGTAG VVPVVPGEVE VVKGQPFDVG PRYTQLQYIG EGAYGMVSSA YDHVRKTRVA IKKISPFEHQ TYCQRTLREI QILLRFRHEN VIGIRDILRA PTLEAMRDVY IVQDLMETDL YKLLKSQQLS NDHICYFLYQ ILRGLKYIHS ANVLHRDLKP SNLLINTTCD LKICDFGLAR IADPEHDHTG FLTEYVATRW YRAPEIMLNS KGYTKSIDIW SVGCILAEML SNRPIFPGKH YLDQLNHILG ILGSPSQEDL NCIINMKARN YLQSLPSKTK VAWAKLFPKS DSKALDLLDR MLTFNPNKRI TVEEALAHPY LEQYYDPTDE PVAEEPFTFD MELDDLPKER LKELIFQETA RFQPGAPEAP
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade.
Gene References into Functions
  1. Retinoic acid was also found to rapidly downregulate extracellular signal-regulated kinase (ERK) 1/2 phosphorylation. PMID: 29520716
  2. Angiostrongylus cantonensis-conditioned culture medium induced suppression of the Erk1/2-NF-kappaB signaling pathway leading to reduced MBP production in RSC96 Schwann cells. PMID: 29742816
  3. Physical exercise promotes proliferation and differentiation of endogenous neural stem cells via ERK in rats with cerebral infarction. PMID: 29901080
  4. Signal communication between neurons and satellite glial cells expands the duration of neuronal ERK1/2 phosphorylation and pain hypersensitivity at 1 h after tissue injury. PMID: 29592783
  5. The experimental results demonstrated that there was a coexistence of GRs and pERK 1/2 in the spinal cord dorsal horn by double immunofluorescence staining. The GR antagonist RU38486 attenuated the morphine analgesia tolerance by inhibiting the expression of GR and increasing the expression of pERK. PMID: 29845273
  6. miRNA-15b deteriorates cardiomyocyte apoptosis by post-transcriptionally downregulating the expression of Bcl-2 and MAPK3. PMID: 28814571
  7. ERK1/2 and Akt phosphorylation were essential for mechano-growth factor E peptide regulating cell morphology and mobility but not proangiogenic capacity of bone marrow-derived mesenchymal stem cells under severe hypoxia PMID: 29441602
  8. Study shows that the mitogen-activated protein kinase pathway is involved in promoting the sodium appetite after low-sodium diet consumption. PMID: 28836382
  9. Homocysteine up-regulates ETA receptor expression via the Sirt1/ERK1/2 signaling pathway in vascular smooth muscle cells. PMID: 28579512
  10. Study demonstrated that blockade of audiogenic seizure (AGS) by inactivation of ERK1/2 inhibits Synapsin I activity and glutamate release in the striatum, decreases the activity of dopaminergic neurons, and leads to upregulation of GABA synthesis in the substantia nigra; suggests that nigrastriatal system may be involved in or affected by the AGS expression in Krushinsky-Molodkina rats. PMID: 28738742
  11. Chemical Constituents from Hericium erinaceus Promote Neuronal Survival and Potentiate Neurite Outgrowth via the TrkA/Erk1/2 Pathway. PMID: 28758954
  12. Phosphorylated extracellular signal-regulated kinase 1/2 blockade in the Locus Coeruleus relieved anxiety and counteracted altered Locus Coeruleus function; authors propose that phosphorylated extracellular signal-regulated kinase 1/2 activation in the Locus Coeruleus plays a crucial role in pain-related anxiety. PMID: 28158734
  13. Apocynin may act as a novel molecular candidate to protect against vascular smooth muscle cell osteogenic switching/vascular calcification through suppressing ERK1/2 pathway. PMID: 27835878
  14. miR-15b-5p might inhibit cell proliferation and induce apoptosis in PC12 cells by targeting ERK1 expression. PMID: 28609839
  15. Late pregnancy is a critical period for changes in phosphorylated mitogen-activated protein kinase/extracellular signal-regulated kinase 1/2 in oxytocin neurones. Results suggest an important role for the MAPK/ERK pathway during reproductive changes in the supraoptic and paraventricular nucleus. PMID: 27203238
  16. EMF-induced cyclic adenosine monophosphate (cAMP) level increase causes protein kinase A (PKA) and extracellular signal-regulated kinase (ERK)1/2 phosphorylation. PMID: 24634418
  17. ERK1/2 kinases in the inferior colliculus of rats genetically prone to audiogenic seizures during postnatal development PMID: 29101749
  18. DOX induced mitochondrial ROS release to activate ERK1/2-mediated HSF2 nuclear translocation and AT1 R upregulation causing DOX-damaged heart failure in vitro and in vivo. PMID: 28295305
  19. CTRP9 regulates hypoxia-mediated human pulmonary artery smooth muscle cell proliferation, apoptosis and migration via TGF-beta1/ERK1/ERK2 signaling pathway. PMID: 28688765
  20. Inhibiting miR-21 attenuates hepatic fibrosis by suppressing both the ERK1 in hepatic stellate cells and epithelial-mesenchymal transition of hepatocyte. PMID: 27226339
  21. Astrocytes can cause Ca(2+) signaling activation, ERK1/2 phosphorylation, and Id4 expression stimulation in oligodendrocyte progenitor cells inducing proliferation of these cells, mainly through Cx47. PMID: 28278052
  22. Neuroprotective mechanism of sevoflurane pre-conditioning might be associated with increased Erk1/2 phosphorylation in mitochondria via inhibition of mPTP opening in the early reperfusion period PMID: 28017206
  23. Rap guanine nucleotide exchange factors Epac1 and Epac2 induces the phosphorylation of extracellular signal-regulated kinases ERK1/2 in pituitary tumor GH3 cells. PMID: 28216156
  24. Alisol A 24-acetate effectively reverses the phenotypic transformation and inhibits the migration of vascular smooth muscle cells, which may be associated with the suppression of the ERK1/2 signaling pathway. PMID: 27924795
  25. Results demonstrated that uric acid can stimulate p38 MAPK, p44/42 MAPK and PDGFRbeta phosphorylation in a time- and concentration-dependent manner. Furthermore, treatment of VSMCs with the angiotensin II type I receptor (AT1R) inhibitor losartan suppressed p38 MAPK and p44/42 MAPK induction by uric acid. PMID: 27400779
  26. Enriched environment could promote rehabilitation after cerebral ischemia via regulation of p-ERK1/2 expression. PMID: 27068861
  27. Paridis rhizoma sapoinins attenuate CCl4-induced liver fibrosis through the regulation of RASAL1/ERK1/2 signal pathway. PMID: 27396351
  28. Data indicate that mitogen activated protein kinases c-Jun N-terminal kinase, p38, and ERK1/2 were activated but with different distributing patterns in the placenta. PMID: 26491907
  29. Data show that N-acetylcysteine modulates mitogen activated protein kinase s ERK1/2 and NF-kappa B signaling pathways in soleus muscle of heart failure (HF). PMID: 27351177
  30. Bisphenol A may promote proliferation of and collagen production by cardiac fibroblasts via activation ERK1/2 signaling pathway. PMID: 27025157
  31. MEK-ERK1-2 pathway is involved in regulating the anti-oxidant strategies to compensate the oxidative status induced by diesel exhaust particle exposure. PMID: 27091075
  32. Erk1 can be considered a proto-oncogene and that Erk molecules possess unusual autoregulatory properties, some of them independent of TEY phosphorylation. PMID: 26658610
  33. The ERK1 in the insular cortex as a part of pathophysiological process that determines predisposition of Krushinsky-Molodkina rats to audiogenic seizures. PMID: 26923581
  34. Beta1 integrin inhibits apoptosis induced by cyclic stretch in annulus fibrosus cells via ERK1/2 MAPK pathway. PMID: 26467923
  35. Surviving CA1 pyramidal cells were identified by hematoxylin and eosin staining and the levels of phosphorylated ERK1/2, P38MAPK and JNK were detected by western blotting. PMID: 26549199
  36. CaSR might play significant roles in the induction of hippocampus apoptosis in rat during hypoxia/reoxygenation through phosphorylation of ERK1/2. PMID: 26617793
  37. TGF-beta1 antagonizes TNF-alpha induced up-regulation of matrix metalloproteinase 3 in nucleus pulposus cells: role of the ERK1/2 pathway. PMID: 26075533
  38. The effects of combinative early and delayed ischemic postconditioning on producing brain BDNF in ischemia may be mediated by the activation of ERK1/2 and CREB. PMID: 26398857
  39. it was shown that HCQ is protective via enhanced phosphorylation of the pro-survival kinase ERK1/2. PMID: 26636577
  40. This study showed that sharp increases in phosphorylated or activated ERK1 was observed at postnatal day (PND) 7 in the SN and VTA. In contrast, in the STR, the levels of phosphorylated ERK1 was significantly higher at postnatal 0 day. PMID: 26363522
  41. inhibition of ERK1/2 activation has no therapeutic effect on kidney fibrosis in SNx possibly due to increased compensatory activation of the p38 and JNK signalling pathways with subsequent upregulation of PAI-1. PMID: 26415098
  42. overexpression of ERp29 in cortical neurons after axotomy increased expression of Erk-1 and PI3K while decreasing the expression of caspase-3 expression. PMID: 25204493
  43. in the native GH3 cell system, ERK and RSK are involved in the pathway linking TRH receptor to r-ERG channel inhibition, but additional components must participate to cause such inhibition. PMID: 26022182
  44. The expression of Cx43 contributes to aldosterone-induced mesangial cell proliferation via the ERK1/2 and protein kinase C pathways. PMID: 26138882
  45. Data show that the simultaneous presence of G-protein-coupled receptor 30 (GPR30) and estrogen receptor alpha (ERalpha) is required for the action of peptide ERalpha17p activation of ERK1/2 (extracellular-signal-regulated kinase 1/2). PMID: 26371374
  46. Enhanced NOX4 expression results in cardiac fibrosis through reactive oxygen species-ERK1/2-MAP kinase-dependent mechanisms in diabetic cardiomyopathy. PMID: 26088607
  47. ERK1/ERK2 activity mainly in the glutamategic neurons of the hippocampus and the temporal cortex is a positive regulator of glutamate release that results in the initiation of auditory seizures. PMID: 26827493
  48. The expression of DUSP6 and DUSP7 is mediated by ERK1/ERK2 activity in microglia. PMID: 26435497
  49. both AKT and ERK1/2 pathways are crucial for the carotid body intracellular signaling process in response to hypoxia. PMID: 26303491
  50. The results are indicative of a novel role of ERK1/2 in glucose-regulated POMC expression and offer new mechanistic insights into hypothalamic glucose sensing. PMID: 25624461
  51. miR-155 plays an important role in regulating the pathological network involving EMT process and ERK1 pathway during hepatic stellate cell activation. PMID: 25142507
  52. ERK1/2 pathway is involved in renal gluconeogenesis inhibition under conditions of lowered NADPH oxidase activity. PMID: 25601753
  53. The present findings support the concept that the pERK1/2 immunofluorescence signal can be used as a quantitative marker for sensitization or inhibition in the pain pathway at spinal and hypothalamic level. PMID: 25497384
  54. Ras, ERK, and Akt are key effectors in the glucocorticoid-induced differentiation of growth hormone-secreting cells. PMID: 24553818
  55. MR-1 attenuated hypoxia/reoxygenation-induced apoptosis by inhibiting the PERK/Nrf2 pathway in neonatal rat cardiomyocytes. PMID: 25542256
  56. Ginsenoside protects against acute renal failure via reduction of renal oxidative stress and enhanced expression of ChAT in the proximal convoluted tubule and ERK1/2 in the paraventricular nuclei. PMID: 24908085
  57. IGF-1 transgene facilitated the differentiation of spinal cord-derived neural stem cells into oligodendrocytes intended for spinal cord injury via the ERK1/2 pathway. PMID: 25162639
  58. Extracellular superoxide dismutase ameliorates streptozotocin-induced rat diabetic nephropathy via inhibiting the ROS/ERK1/2 signaling. PMID: 26006040
  59. Activation of the ERK1/2 signalling pathway, increased phosphorylation of c-myc and significantly increased expression of cyclin D2 protein, are demonstrated. PMID: 25450615
  60. Sevoflurane post-conditioning protects isolated rat hearts against myocardial I/R injury and inhibits cell oncosis and apoptosis via activation of the ERK1/2 pathway. PMID: 25345742
  61. Ang-(1-7) abolished advanced glycated end product-induced activation of the MAP kinase ERK1/2. PMID: 25246357
  62. The necroptotic cell death mechanism is executed by an ERK1/ERK2-RIP3 pathway in the retinal ganglion cells in early stages after ischemia-reperfusion injury. PMID: 25352744
  63. Data showw that blocking of AT2 receptors and inhibition of either endothelial nitric oxide synthase (eNOS) or extracellular signal-regulated protein kinases 1 and 2 (ERK1/2) restored Ang II-induced contraction in obese rats. PMID: 25170617
  64. activation of ERK1/2 is a key event in the induction of luteal cell autophagy during corpus luteum regression which is not associated with mTOR regulation. PMID: 25107837
  65. ERK activation is sufficient to reduce SERCA2 mRNA. PMID: 25008120
  66. Hypoxia-induced endothelial proliferation and migration, an important underlying mechanism contributing to hypoxic pulmonary vascular remodelling, appears to be mediated by 15-PGDH and 15-KETE, via the ERK1/2 signalling pathway PMID: 24467360
  67. Down-regulation of HDAC1 and ERK1/ERK2 accelerates senescence of transformed rat embryo fibroblasts. PMID: 25697003
  68. Our results demonstrated that the activation of spinal chemokine receptor CXCR3 mediated bone cancer pain through Akt and ERK 1/2 kinase PMID: 25281485
  69. Shock wave treatment induced ATP release, increased Erk1/2 and p38 MAPK activation, and enhanced proliferation in three different cell types PMID: 25118288
  70. These data indicate that dentin matrix proteins promote the dentinogenic/osteogenic differentiation of bone marrow stromal stem cells via the ERK/p38 MAPK pathways. PMID: 24562313
  71. these findings suggested that p-Cdk5(S159) regulated by ERK pathway activity may be a critical mechanism involved in the activation of Cdk5 in nociceptive spinal neurons contributes to peripheral inflammatory pain hypersensitivity. PMID: 24498195
  72. Acute and subchronic stress increases phospho (p)-ERK, p-CREB, and c-fos levels in the mesocorticolimbic system. PMID: 24292370
  73. In cortical neurons hypoxia differentially upregulated the phosphorylation activation states of MAPK(p38) and MAPK(ERK-1/2 (p44/p42). PMID: 24555431
  74. Reductions in GluN2A and GluN2B subunits of NMDA receptors coupled with altered MAPK signaling, in response to inflammatory stimuli may be related to the cognitive deficits observed in Alzheimer's disease. PMID: 24761931
  75. PHO-ERK1/2 interaction with mitochondria regulates the permeability transition pore in cardioprotective signaling PMID: 24835217
  76. PERK pathway of the unfolded protein response (UPR) is involved in glucose deprivation (GD)-induced apoptotic neuronal death through the activation of caspase-12, rather than the mitochondrial-dependent caspase pathway. PMID: 24185830
  77. Isosexual social interaction induces rapidly pERK expression in the main olfactory epithelial related brain. PMID: 24001592
  78. OPG regulated the proliferation of chondrocytes via MEK/ERK signaling, and directly affected chondrocytes by influencing the expression profile of ADAMTS-5 and TIMP-4. PMID: 24126801
  79. C3G acts as a pro-survival molecule in H9C2 cardiomyocytes by activation of phosphorylated-ERK1/2. PMID: 23686869
  80. It is an apoptotic protein and may play partial role in mediating anti apoptotic effect of genistein. PMID: 24117073
  81. ERK1/2 activation in dorsal horn nociceptive neurons may be linked to the development of hyperalgesia. PMID: 23880531
  82. IGF-1 can protect primary cultured retinal ganglion cells against hypoxia-induced apoptosis via the Erk-1/2 and Akt signaling pathways. PMID: 24049436
  83. blocking ERK1/2 phosphorylation in the glials blocked Nav 1.7 up-regulation in the neurons and correspondingly attenuated the hyperalgesia of the inflamed temporomandibular joint PMID: 23242737
  84. Data suggest that ERK1/2 inhibition could provide some protection against the adverse effects of IL-1. PMID: 23680829
  85. Caveolin-1 and IQGAP1 are required for phosphorylation of the actin associated pool of extracellular signal regulated kinase 1 and 2 (ERK1/2) in response to protein kinase C activation. PMID: 23987506
  86. results identify STYX as an important regulator of ERK1/2 signaling critical for cell migration PMID: 23847209
  87. The present study indicates that the transient activation of spinal ERK1/2 contributes to the initiation of pain hypersensitivity following surgical incision. PMID: 23450427
  88. TGFbeta induces signaling involving PI3kinase-dependent Mnk-1-mediated phosphorylation of eIF4E at Ser-209 to facilitate mesangial cell hypertrophy. A role for dissociation of 4EBP-1-eIF4E complex for Mnk-1-mediated phosphorylation of eIF4E. PMID: 23359369
  89. Findings suggest a mechanism in which NMDAR-dependent phosphorylation of ERK1/2 modulates satiation signals via synapsin I phosphorylation in vagal afferent endings. PMID: 23715865
  90. Data suggest that ERK1/2 has a signaling role in the regulation of proliferation of 8 cell types during liver regeneration process. PMID: 23546948
  91. Candidate ERK1/2 substrates are involved in diverse cellular processes including transcriptional regulation, chromatin remodeling, RNA splicing, cytoskeleton dynamics, cellular junctions and cell signaling. PMID: 23712012
  92. The cardioprotection afforded by sabiporide is attributed in part to inhibition of ERK1/2 phosphorylation and suppression of iNOS expression. PMID: 23484128
  93. Data show that pinocembrin inhibits AngII-induced aortic ring contraction by suppression of the increase in [Ca(2+)]i and ERK1/2 activation via blocking AT1R. PMID: 23611777
  94. Data indicate that class I HDACs function as signal-dependent repressors of cardiac hypertrophy via inhibition of the gene encoding dual-specificity phosphatase 5 (DUSP5) DUSP5, a nuclear phosphatase that negatively regulates signaling by ERK1/2. PMID: 23720316
  95. PACAP promotes both survival and neuritogenesis in PC12 cells by activating NF-kappaB pathway, most likely via classical and alternative signaling cascades involving ERK1/2 kinases, Ca(2+), and c-Rel/p52 dimers. PMID: 23564451
  96. these results indicate that p44/42 MAPK is required for AngII-induced sodium appetite but not thirst or neurohypophysial secretion. PMID: 22913624
  97. mGluR4 promotes the proliferation of rat neural progenitor cells and cyclin D1 expression through activation of ERK1/2 signaling pathways in vitro. PMID: 23374450
  98. desipramine-increased HO-1 expression is mediated by Nrf2 activation through the ERK and JNK signaling pathways PMID: 23209658
  99. ERK1/2 are differentially activated in the spinal cord and in selected brainstem nuclei implicated in nociception, in response to an acute noxious stimulus and/or to a neuropathic pain condition. PMID: 23055268
  100. Tyrosine 321 of GIT1 is a critical phosphorylation site for GIT1 interaction with ERK1/2, regulation of ERK1/2 activation, VEGF expression and angiogenesis at the fracture site. PMID: 22797318

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Subcellular Location Cytoplasm, Nucleus, Membrane, caveola
Protein Families Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
Tissue Specificity Highest levels within the nervous system, expressed in different tissues, mostly in intestine, placenta and lung.
Database Links

KEGG: rno:50689

STRING: 10116.ENSRNOP00000026627

UniGene: Rn.2592

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