Recombinant Rat Myosin light chain kinase 2, skeletal/cardiac muscle (Mylk2)

Code CSB-YP321833RA
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP321833RA
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP321833RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP321833RA
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP321833RA
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
Mylk2
Uniprot No.
Alternative Names
Mylk2; Myosin light chain kinase 2; skeletal/cardiac muscle; MLCK2; EC 2.7.11.18
Species
Rattus norvegicus (Rat)
Expression Region
2-610
Target Protein Sequence
ATENGAVEL GTQSLSTDHP PTDAAGDGSP ASEKEPSLPD TEKDLGPTNT KKDPGAPDPK KNPDPPSLKK TPEAPGPEKK GDSAPASASN QGPSGEGDGG GGPAEGGTGP PAVLPQPTAT ADASIQKLDA TQAPSGNQES GEAKAGKKAA ECREAGRRGS PAFLHSPSCP AIISCSEKTL AMKPLSETTE LIFAGVSETP DPQDPGPAKD EGGTNTLADG KEEAEAGQAE QAKVQGDTSQ RIGFQAVPSE RAEVGQALCL TAKEEDCFQI LDDCPPPPAP FPHRIVELRT GNVSSEFSMN SKEALGGGKF GAVCTCTERS TGLKLAAKVI KKQTPKDKEM VLLEIEVMNQ LNHRNLIQLY SAIETSHEII LFMEYIEGGE LFERIVDEDY QLTEVDTMVF VRQICDGILF MHKMRVLHLD LKPENILCVN TTGHLVKIID FGLARRYNPN EKLKVNFGTP EFLSPEVVNY DQISDKTDMW SLGVITYMLL SGLSPFLGDD DTETLNNVLS ANWYFDEETF EAVSDEAKDF VSNLITKDQS ARMSAEQCLA HPWLNNLAEK AKRCNRRLKS QILLKKYLMK RRWKKNFIAV SAANRFKKIS SSGALMALGV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Implicated in the level of global muscle contraction and cardiac function. Phosphorylates a specific serine in the N-terminus of a myosin light chain.
Gene References into Functions
  1. MLCK regulates Schwann cell cytoskeletal organization, cell differentiation and myelination. PMID: 22100921
  2. Rho-kinase and MLC kinase play a role in migratory defect in FAK null cells. PMID: 12105199
  3. in rat skeletal muscles an increase of MLC2 phosphorylation is associated with the slow-to-fast transition regardless of whether hypertrophy or atrophy develops. PMID: 12748068
  4. role of myosin phosphorylation by myosin light chain kinase (MLCK) in regulating the invasiveness of metastatic cancer cells PMID: 12970723
  5. Elk-1 regulates telokin expression PMID: 16260603
  6. These data provide novel insight into the regulation of smooth muscle myosin light chain kinase (smMLCK) expression at the molecular level and demonstrate the importance of serum response factor (SRF) in regulating smMLCK promoter activity. PMID: 16822834
  7. ML-7 and apocynin blocked hypoxia-dependent phosphorylation of MLC. Our data demonstrate that hypoxia causes a breakdown of the BBB in vitro and in vivo involving ROS and the contractile machinery. PMID: 17419808
  8. AG repeats in the spontaneously hypertensive rats promoter increase H-DNA structures, histone modifications, and promoter activity of the smooth muscle myosin light chain kinase, perhaps contributing to vascular disorders in hypertension PMID: 17991897
  9. The regulation of smMLCK expression by angiotensin II via Ras signaling is important in the regulation of vascular remodeling and blood pressure PMID: 18511912
  10. PKC and MLCK pathways important in coronary endothelial dysfunction elicited by ischemia/reperfusion injury and cytokine release PMID: 18621396

Show More

Hide All

Subcellular Location
Cytoplasm.
Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family
Database Links
icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1