Recombinant Rat Nuclear pore complex protein Nup54 (Nup54)

Code CSB-YP016203RA
MSDS
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Source Yeast
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Code CSB-EP016203RA
MSDS
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Source E.coli
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Code CSB-EP016203RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP016203RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP016203RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nup54
Uniprot No.
Alternative Names
Nup54; Nuclear pore complex protein Nup54; 54 kDa nucleoporin; Nucleoporin Nup54
Species
Rattus norvegicus (Rat)
Expression Region
1-510
Target Protein Sequence
MAFNFGAPSG TSGTSTATAA PAGGFGGFGT TTTTAGSAFS FSAPTNTGST GLLGGTQNKG FGFGTGFGTS TGTGTGLGTG LGTGLGFGGF NTQQQQQQQQ TSLGGLFSQP AQAPAQSNQL INTASALSAP TLLGDERDAI LAKWNQLQAF WGTGKGYFNN NIPPVEFTQE NPFCRFKAVG YSCMPNNKDE DGLVVLIFNK KETDIRSQQQ QLVESLHKVL GGNQTLTVNV EGIKTLPDDQ TEVVIYIVER SPNGTSRRVP ATTLYAHFEQ ANIKTQLQQL GVTLSMTRTE LSPAQIKQLL QNPPAGVDPI IWEQAKVDNP DSEKLIPVPM VGFKELLRRL KVQDQMTKQH QTRLDIISED ISELQKNQTT TMAKIAQYKR KLMDLSHRTL QVLIKQEIQR KSGYAIQAEE EQLRVQLDTI QGELNAPTQF KGRLNELMSQ IRMQNHFGAV KSEEKYYIDA DLLREIKQHL KQQQEGLSHL ISIIKDDLED IKLVEHGLNE TIHSRGGVFS
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the nuclear pore complex, a complex required for the trafficking across the nuclear membrane.
Gene References into Functions
  1. we carried out solution analysis utilizing the entire ordered regions of Nup62, Nup54, and Nup58, and demonstrate that they form a dynamic "triple complex" PMID: 26025361
  2. A 1:1:1 stoichiometry of the Nup62 complex, where Nup54 is central with direct binding to Nup62 and Nup58. PMID: 24574455
  3. Study reports the crystal structures of the Nup54-Nup58 and Nup62-Nup54 interacting domains and reconstruct the molecular architecture of the NPC transport channel. PMID: 22036567
Subcellular Location
Nucleus, nuclear pore complex. Nucleus membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus membrane; Peripheral membrane protein; Nucleoplasmic side. Note=Biased towards cytoplasmic side. Central region of the nuclear pore complex, within the transporter.
Protein Families
NUP54 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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