Recombinant Rat Pancreatic secretory trypsin inhibitor (Spink1)

Code CSB-YP022575RA
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Source Yeast
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Code CSB-EP022575RA
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Source E.coli
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Code CSB-EP022575RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP022575RA
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Source Baculovirus
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Code CSB-MP022575RA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Spink1
Uniprot No.
Alternative Names
Spink1; Serine protease inhibitor Kazal-type 1; Cholecystokinin-releasing peptide; Monitor peptide; Pancreatic secretory trypsin inhibitor; PSTI-I
Species
Rattus norvegicus (Rat)
Expression Region
19-79
Target Protein Sequence
GN PPAEVNGKTP NCPKQIMGCP RIYDPVCGTN GITYPSECSL CFENRKFGTS IHIQRRGTC
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Serine protease inhibitor which exhibits anti-trypsin activity. In the pancreas, protects against trypsin-catalyzed premature activation of zymogens.; In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production.
Gene References into Functions
  1. This study created a novel mouse model of mtDNA depletion by expressing relatively low levels of mito-PstI in CNS neurons, which leads to a chronic OXPHOS deficiency. PMID: 21734281
  2. severity of chronic pancreatitis and pancreatic fibrosis is significantly reduced in mice expressing rat PSTI-I; propose that endogenous pancreatic trypsin inhibitors play a protective role in the pancreatic response to repeated injurious events PMID: 19904222
  3. dual function: monitoring protein in gut due to its 'moderate' affinity for trypsin and a protective role in pancreas PMID: 12771515
  4. Partially folded bovine pancreatic trypsin inhibitor analogues attain fully native structures when co-crystallized with S195A rat trypsin. PMID: 18054043
  5. Caltrin protein modulates the acrosomal exocytosis during sperm capacitation. PMID: 18550793

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Subcellular Location
Secreted.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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