Recombinant Rat Phospholipase D1(Pld1) ,partial

Code CSB-YP018144RA
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Source Yeast
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Code CSB-EP018144RA
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Source E.coli
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Code CSB-EP018144RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018144RA
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Source Baculovirus
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Code CSB-MP018144RA
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names Pld1
Uniprot No. P70496
Alternative Names Pld1; Phospholipase D1; PLD 1; rPLD1; EC 3.1.4.4; Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1
Species Rattus norvegicus (Rat)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic (By similarity).
Gene References into Functions
  1. These results suggest that PLD1 activity regulates COPII vesicle transport from the endoplasmic reticulum (ER) to the Golgi apparatus by regulating Sec13/31 recruitment from the cytosol to the ER membrane during COPII vesicle formation. PMID: 28648601
  2. During neuronal differentiation of neural stem cells, Pld1 is activated. PMID: 24986006
  3. PLD plays an important role in the development and progression of rat liver fibrosis PMID: 24728967
  4. Phospholipase D1 (PLD1)-mediated signalling, that may involve 5-HT2A receptor (5-HT2AR) activation, at both levels of the nervous system, is reported. PMID: 23932932
  5. These results suggest that PLD1 is an important regulator of bFGF-induced NT-3 expression and neurite outgrowth. PMID: 22544632
  6. these results suggest that RhoA-PLD1- phosphatidic acid may represent a novel signaling pathway in the restriction of dendritic branching and may thus provide insight into the mechanisms of dendritic morphogenesis PMID: 22674271
  7. This study evaluated whether overexpressed Bcl-2 influences neurite outgrowth in immortalized hippocampal progenitor cells. PMID: 21916846
  8. new insights in the intricate way PLD and mTOR cooperate to control myogenesis PMID: 21525000
  9. These results indicate that inhibition of PLD activity by CHS-111 is attributable to the blockade of RhoA activation via multiple steps in the signalling cascade. PMID: 20965153
  10. the peak level of PLD1 was detected in the cerebrum at 21 days postnatally and declined progressively thereafter. PMID: 19010519
  11. mechanism for the gating of the ubiquitous TRPC3 channel and a key role for phospholipase D1 in the generation of the slow excitatory postsynaptic current in cerebellar Purkinje cells PMID: 19741172
  12. Expression and characterization of rat brain phospholipase D1 PMID: 11665609
  13. Mechanisms of regulation of phospholipase D1 and D2 by the heterotrimeric G proteins G13 and Gq PMID: 11812783
  14. activation of PLD1b results from phosphatidylinositol 3-kinase-dependent stimulation of Rac1, ARF6, and PKCalpha. PMID: 11950936
  15. stimulated cell protrusion in V-Src transformed cells PMID: 12054584
  16. gene expression and stability in primary astrocytes PMID: 12270129
  17. Data show that phospholipases D1 and D2 (PLD1 and PLD2) are expressed in mast cells; PLD1 localized to intracellular vesicles and remained in this location after stimulation with antigen PMID: 12388770
  18. Data show that epidermal growth factor (EGF)-induced phospholipase D1 (PLD1) phosphorylation and activation occur in the caveolin-enriched membrane (CEM) and that localization of PLD1 to the CEM via palmitoylation is critical for EGF signaling. PMID: 12429840
  19. PLD1 expression is regulated by protein kinase C alpha and requires both the regulatory and catalytic domains of PKCa PMID: 12431982
  20. PLD1 in macrophages and Schwann cells plays an important role in the activation of these cells in the pathogenesis of experimental autoimmune neuritis, an animal model of human peripheral demyelinating disease. PMID: 12472177
  21. elevated PLD activity generates survival signals allowing cells to overcome default apoptosis programs PMID: 12615079
  22. PC12 cells require PLD1 to attenuate glutamate-induced cell death. PMID: 12642902
  23. Pld1 localization and function involve regulated and continual cycling through a succession of subcellular sites, mediated by successive combinations of membrane association interactions. PMID: 12876278
  24. PLD has a role in preventing vascular smooth muscle cell apoptosis PMID: 14660552
  25. LPA activates protein translation through the action of PLD1-generated phosphatidic acid on mTOR and the PI3K/Akt pathway whereas PDGF acts through P13K/Akt independent of PLD1 PMID: 14769825
  26. PLD1 is essential for the regulated secretion of insulin from pancreatic beta-cells. PMID: 15087463
  27. interaction of PKCalpha with PLD1 in the perinuclear region is required for PLD1 activation PMID: 15187091
  28. survival signals generated by PLD1 suppress DNA damage-induced increases in p53 stabilization and cause increased expression of MDM2 and increased turnover of p53 PMID: 15199126
  29. phospholipase D isozymes mediate EGCG-induced COX-2 expression through PKC and p38 in immortalized astroglial line and normal astrocyte cells PMID: 15210717
  30. results suggest that interaction of the phospholipase D1(PLD1) PX domain with phosphatidylinositol 3,4,5-trisphosphate and/or phosphatidic acid (or phosphatidylserine) may be an important factor in the spatiotemporal regulation and activation of PLD1 PMID: 15475361
  31. differential changes in PLD isozymes may contribute to the pathophysiology of congestive heart failure PMID: 15601581
  32. PLD participates in myogenesis through phosphatidic acid- and PIP2-dependent actin fiber formation PMID: 15616193
  33. In conclusion, this is the first evidence to show that PLD1 acts as an important regulator of neurite outgrowth in neural stem cell by promoting neuronal differentiation via increase of synapsin I expression. PMID: 15752728
  34. RalA controls exocytosis of large dense core secretory granules by interacting with ARF6-dependent phospholipase D1. PMID: 15980073
  35. phospholipase D1 is allosterically modulated by monomeric GTPases, protein kinase C, and polyphosphoinositides PMID: 16339153
  36. active state of cell division cycle 42 is important in upregulation of PLD activity which is responsible for the increase of neurite outgrowth PMID: 16842757
  37. Pld1 activation through Src and Ras is involved in bFGF-induced neurite outgrowth of hippocampus neurons. PMID: 17146759
  38. PLD1 short interference RNA-mediated inhibition of exocytosis can be rescued by exogenous provision of a lipid that favors the transition of opposed bi-layer membranes to hemifused membranes. PMID: 17540765
  39. disruption of the PED/PEA-15-PLD1 molecular interaction enhances insulin sensitivity in skeletal muscle cells PMID: 18541525
  40. RSK2 is an essential modulator of PLD1 activity in calcium-regulated exocytosis. PMID: 18550821
  41. RSK2 is a critical upstream signaling element in the activation of PLD1 to produce the lipids required for exocytosis PMID: 19161391
  42. betaPix is identified as the guanine nucleotide-exchange factor integrating Rac1 activation to PLD1 and the exocytotic process. PMID: 19261846
  43. these findings suggest that PLD1 and PLD2 play an important role in promoting IgE-dependent signaling events within lipid microdomains in mast cells. PMID: 19794068

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Subcellular Location Cytoplasm, perinuclear region, Endoplasmic reticulum membrane, Lipid-anchor, Golgi apparatus membrane, Lipid-anchor, Late endosome membrane, Lipid-anchor
Protein Families Phospholipase D family
Database Links

KEGG: rno:25096

STRING: 10116.ENSRNOP00000034466

UniGene: Rn.11130

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