Recombinant Rat Phospholipase D2 (Pld2), partial

Code CSB-YP018145RA
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Source Yeast
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Code CSB-EP018145RA
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Source E.coli
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Code CSB-EP018145RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP018145RA
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Source Baculovirus
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Code CSB-MP018145RA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pld2
Uniprot No.
Alternative Names
Pld2; Phospholipase D2; PLD 2; rPLD2; EC 3.1.4.4; Choline phosphatase 2; PLD1C; Phosphatidylcholine-hydrolyzing phospholipase D2
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Function as phospholipase selective for phosphatidylcholine. May have a role in signal-induced cytoskeletal regulation and/or endocytosis.
Gene References into Functions
  1. Phosphatidic acid produced by PLD2 plays an important role in regulating cell morphology in mast cells. PMID: 22344748
  2. A high level of cell invasiveness of cancer cells can be explained for the first time by combined high JAK3/PLD2 phosphorylation and activity involving PLD2's Y415 residue, which might constitute a novel target to inhibit cancer cell invasion. PMID: 21414324
  3. Data demonstrate that alpha-syn is an inhibitor of PLD2 in vivo. PMID: 20664530
  4. Phospholipase D2-dependent inhibition of the nuclear hormone receptor PPARgamma by cyclic phosphatidic acid. PMID: 20705243
  5. The level of PLD2 in both the cerebrum and hindbrain was minimal compared to that of PLD1. PMID: 19010519
  6. ARF6 protein regulates mu-opioid receptor trafficking and signaling via PLD2 activation and hence affects the development of opioid receptor desensitization and tolerance. PMID: 19666113
  7. PKC delta-dependent direct phosphorylation plays an important role in the regulation of PLD2 activity in PC12 cells. PMID: 11744693
  8. interaction with aldolase via PH domain PMID: 11876650
  9. stimulates cell protrusion in V-Src transformed cells PMID: 12054584
  10. gene expression and stablity in primary astrocytes PMID: 12270129
  11. Data show that phospholipases D1 and D2 (PLD1 and PLD2) are expressed in mast cells and that PLD2 localizes to membrane ruffles of antigen-stimulated mast cells together with endogenous ADP ribosylation factor 6 (ARF6). PMID: 12388770
  12. demonstrate a significant fraction of endogenous PLD2 localized to the perinuclear Golgi region and was also distributed throughout cells in dense cytoplasmic puncta; data are consistent with a model whereby PLD2 plays a role in Golgi vesicular transport PMID: 12429836
  13. PC12 cells require PLD2 to attentuate glutamate-induced cell death. PMID: 12642902
  14. PLD2 has a role in activation of phospholipase C gamma 1 and is important for its activity PMID: 12646582
  15. H2O2 modifies GAPDH on its catalytic cysteine residue not only to inactivate the dehydrogenase activity of GAPDH but also to endow GAPDH with the ability to bind PLD2 and the resulting association is involved in the regulation of PLD2 activity by H2O2 PMID: 12753082
  16. direct evidence for linkages between PLD2, ROS production, and S100B-RAGE-induced enhancement of Ang II-induced cell proliferation and activation of JAK2 in VSMCs. PMID: 12941779
  17. PLD2 functions at the plasma membrane to facilitate endocytosis of the angiotensin II type 1 receptor. PMID: 14718562
  18. PLD2 expression in choroid plexus and ventricular zone of lateral and third ventricles at embryonic day 15 and also in developing cerebral cortex and hippocampus suggests PLD2 involvement in early developmental processes of some neuronal progenitors. PMID: 15036596
  19. Interaction with dynamin may be involved in enzyme activation. PMID: 15581494
  20. These data suggest that the ANG II-induced neointimal growth is mediated by the activation of Akt through a mechanism dependent on cPLA2 and PLD2 activation in balloon-injured rat carotid arteries. PMID: 16024570
  21. Antigen stimulation resulted in increased association and colocalization of Syk with PLD2 on the plasma membrane, an early critical event in the activation of mast cells. PMID: 16861349
  22. First demonstration that PLD2 activation is involved in GAP-43 and synapsin I expression during depolarization-induced neuronal differentiation in PC12 cells. PMID: 18004999

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Subcellular Location
Cell membrane; Lipid-anchor.
Protein Families
Phospholipase D family
Tissue Specificity
Expressed in brain, lung, heart, kidney, stomach, small intestine, colon, and testis, and at a much lower levels in thymus, liver and muscle.
Database Links

KEGG: rno:25097

UniGene: Rn.9798

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