Recombinant Rat Platelet endothelial cell adhesion molecule (Pecam1), partial

Code CSB-YP668776RA
MSDS
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Source Yeast
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Code CSB-EP668776RA
MSDS
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Source E.coli
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Code CSB-EP668776RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP668776RA
MSDS
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Source Baculovirus
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Code CSB-MP668776RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Pecam1
Uniprot No.
Alternative Names
Pecam1; Pecam; Platelet endothelial cell adhesion molecule; PECAM-1; CD antigen CD31
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-660 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis.
Gene References into Functions
  1. Circulating PECAM-1 is a promising biomarker for the detection and monitoring of ventilator-induced lung injury. PMID: 24588994
  2. Shock wave therapy leads to mobilization of endogenous CD31/CD34-positive endothelial cells. PMID: 23395097
  3. PECAM-1 expression on PMNs is in a converse way between pancreatic microcirculation and peripheral circulation in acute edematous pancreatitis PMID: 14599961
  4. A reverse expression of PECAM-1 on neutrophils was found between peripheral circulation and pancreatic microcirculation, so inhibition of PECAM-1 expression may improve the pathological change of acute edematous pancreatitis. PMID: 15655817
  5. expression pattern of PECAM & VE-cadherin at endothelial cell junctions in adult initial lymphatic network; results suggest that primary valves are associated with discontinuous expression of endothelial junction molecules PMID: 17935476

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Subcellular Location
Cell membrane; Single-pass type I membrane protein. Membrane raft. Cell junction.
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