Recombinant Rat Protein polyglycylase TTLL10 (Ttll10)

Code CSB-YP719869RA
MSDS
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Source Yeast
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Code CSB-EP719869RA
MSDS
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Source E.coli
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Code CSB-EP719869RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP719869RA
MSDS
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Source Baculovirus
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Code CSB-MP719869RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Ttll10
Uniprot No.
Alternative Names
Ttll10; Protein polyglycylase TTLL10; EC 6.3.2.-; Tubulin--tyrosine ligase-like protein 10
Species
Rattus norvegicus (Rat)
Expression Region
1-679
Target Protein Sequence
MPLHPPARRP HGHRRNGSEA QTEATTQDTG RLSCPRRVGA AVCPIQGLGH RAARRPRRGV GTTSASRAPR PGALMPATRN RPRFIHYRVQ PPRTHVSFKR PKRSRTHQSH TKVPGWTHEK RMGSSVEEGL RPELSQLEQD ADNLGEEEAA RLPVTSLNGL LMEGDRHPNP GQGPFFYIGG TNGASIISNY CESKGWQRTQ DSRCEDYKLK WCEIKCRDNY CSFREGQQLL FQLPNNKLLT TKIGLLSALR EHARTLSKAR LMPSTQAKVL KMDEFFPETY RLDIRDERQA FFTLFDETQM WICKPTASNQ GKGIFLIRSQ EEAAALQAKT QSIEDDPIYR KMPFRAPQAR VVQRYVQNPL LLDGKKFDVR SYMLIACAMP YMVFFGHGYA RLTLSLYNPH SSDLSGHLTN QFMQKKSPLY MLLKDSTVWS MEHLNRYIND KFRKSKGLPR DWVFTTFTKR MQQIMSHCFL AVKSKLECKL GYFDLIGCDF LIDENFKVWL LEMNSNPALH TNCEVLKEVI PGVVMETLDL ALETCQKSLH SQKMLPLQSQ RRFVLLYNGE TTDLWPRLGS SRPPIRLPYA NSNHARSTCE ISSSSLTSTR VTIADRPAAR KSMSSRTAPI CASRKSRLSD SGGVSIEESE TSVCSGLPEG SRDAAREPSL GPTEEEREEE QRSTSHRGS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Polyglycylase which modifies both tubulin and non-tubulin proteins, generating polyglycine side chains of variable lengths on the gamma-carboxyl groups of specific glutamate residues of target proteins. Involved in the elongation step rather than the initiation step of the polyglycylation reaction. Polyglycylates alpha-tubulin and beta-tubulin. Polyglycylates non-tubulin proteins such as nucleosome assembly protein NAP1.
Subcellular Location
Cytoplasm, cytoskeleton. Cell projection, cilium. Cytoplasm, cytoskeleton, cilium axoneme.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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