Product Details

Purity

>85% (SDS-PAGE)

Target Names

Rest

Uniprot No.

O54963

Alternative Names

Rest; Nrsf; RE1-silencing transcription factor; Neural-restrictive silencer factor

Species

Rattus norvegicus (Rat)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, SIN3A and RCOR1, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. Transcriptional repression by REST-CDYL via the recruitment of histone methyltransferase EHMT2 may be important in transformation suppression. Represses the expression of SRRM4 in non-neural cells to prevent the activation of neural-specific splicing events and to prevent production of REST isoform 6. Repressor activity may be inhibited by forming heterodimers with isoform 6, thereby preventing binding to NRSE or binding to corepressors and leading to derepression of target genes. Also maintains repression of neuronal genes in neural stem cells, and allows transcription and differentiation into neurons by dissociation from RE1/NRSE sites of target genes. Thereby is involved in maintaining the quiescent state of adult hippocampal neural stem cells and preventing premature differentiation into mature neurons. Plays a role in the developmental switch in synaptic NMDA receptor composition during postnatal development, by repressing GRIN2B expression and thereby altering NMDA receptor properties from containing primarily GRIN2B to primarily GRIN2A subunits. Acts as a regulator of osteoblast differentiation. Key repressor of gene expression in hypoxia; represses genes in hypoxia by direct binding to an RE1/NRSE site on their promoter regions. May also function in stress resistance in the brain during aging; possibly by regulating expression of genes involved in cell death and in the stress response. Repressor of gene expression in the hippocampus after ischemia by directly binding to RE1/NRSE sites and recruiting SIN3A and RCOR1 to promoters of target genes, thereby promoting changes in chromatin modifications and ischemia-induced cell death. After ischemia, might play a role in repression of miR-132 expression in hippocampal neurons, thereby leading to neuronal cell death.; Binds to the 3' region of the neuron-restrictive silencer element (NRSE), with lower affinity than full-length REST isoform 1. Exhibits weaker repressor activity compared to isoform 1. May negatively regulate the repressor activity of isoform 1 by binding to isoform 1, thereby preventing its binding to NRSE and leading to derepression of target genes. However, in another study, does not appear to be implicated in repressor activity of a NRSE motif-containing reporter construct nor in inhibitory activity on the isoform 1 transcriptional repressor activity. Post-transcriptional inactivation of REST by SRRM4-dependent alternative splicing into isoform 6 is required in mechanosensory hair cells in the inner ear for derepression of neuronal genes and hearing.

Gene References into Functions

Authors demonstrate for the first time novel neuroprotective function of REST in prion diseases and hypothesise that the LRP6-Wnt-beta-catenin/REST signaling plays critical and collaborative roles in neuroprotection. PMID: 26919115
miR-20, Rest and Wnt signaling are suggested to be involved in a regulatory circuit that can modulate the neural differention of neural progenitor cells. [mir-20] PMID: 26996236
These effects of FosDT in part are due to its interactions with corepressors of the transcription factor REST and subsequent derepression of REST-downstream genes PMID: 26674869
The transcription factor NRSF contributes to epileptogenesis by selective repression of a subset of target genes. PMID: 25117540
Findings indicate a role for RE1-silencing transcription factor REST-dependent repression of miR-132 in the neuronal death associated with global ischemia. PMID: 25108103
REST is essential for experience-dependent fine-tuning of genes involved in synaptic plasticity. PMID: 22960932
NRSF may be the key gene during the differentiation from bone marrow mesenchymal stem cells to neurons. PMID: 20078938
Data show that Egr1-induced activation of the Ca(V)3.2 promoter was effectively counteracted by the repressor element 1-silencing transcription factor (REST). PMID: 22431737
Data show that neuronal insults trigger activation of REST and CoREST in a clinically relevant model of ischemic stroke and that REST binds a subset of transcriptionally responsive genes. PMID: 22371606
REST/NRSF governs the expression of dense-core vesicle gliosecretion in astrocytes PMID: 21536750
REST is required for the differentiation of oligodendrocyte precursor cells (OPC) into oligodendrocytes and may play important roles in regulating OPC development and plasticity. PMID: 21368059
In wild-type PC12 transfected with REST constructs, expression of genes encoding proteins of dense-core and synaptic-like vesicles is decreased. PMID: 21046448
The expression of enlargeosomes, their exocytosis at growth cones and their form of neurite outgrowth are positively governed by the RE-1 silencing transcription factor. PMID: 20604903
The predominant expression of REST4, HuB/C/D and nPTB in post-mitotic fiber cells, together with miR-124 expression in vertebrate lenses, was shown. PMID: 21139978
These results suggest that the activation of REST4-mediated gene regulation in the medial prefrontal cortex during postnatal development is involved in stress vulnerability. PMID: 21068306
differential repression of Ca2+ signalling in pheochromocytoma PC12 cells PMID: 20171735
silencing of neuronal-specific genes by REST requires the recruitment of an associated corepressor, CoREST. PMID: 12399542
Global ischemia triggers REST mRNA and protein expression; REST suppresses GluR2 promoter activity and gene expression in neurons destined to die PMID: 12657670
the transcriptional repressor NRSF/REST regulates connexin36 PMID: 14565956
These results suggest that ethanol-induced potentiation of NRSF binding activity underlies the mechanism of ethanol inhibition of neuronal differentiation and decreased neurogenesis. PMID: 15959844
Data suggest that REST/NRSF contains a NLS around ZFD-5, while the putative NLS at residues 512-522 is non-functional. PMID: 16442230
REST and REST4 alter the expression of proconvulsant genes and may regulate the progression of epileptogenesis PMID: 16828291
Many repressed genes recruit recombinant REST protein to repressor element 1 sites within their promoters, and abrogation of REST function leads to reactivation of these transcripts. PMID: 16945103
Our work identifies one of the mechanisms underlying the phenotype of neurosecretory-deficient neuroendocrine cells, and begins to define the critical components that determine a key aspect of the neuroendocrine phenotype. PMID: 17064356
Data show that continued REST expression prevents increases in the levels of the BNP (Nppb) and ANP (Nppa) genes, encoding brain and atrial natriuretic peptides, in adult rat ventricular myocytes in response to endothelin-1. PMID: 17371849
REST protein is the factor governing the maintenance of the neurosecretory process and the properties of dense-core vesicles in PC12 cells. PMID: 18298477
Cocaine- and amphetamine-regulated transcript (CART) gene expression in neuroendocrine cells is strictly controlled by neuron-restrictive silencer element (NRSE/REST), via a mechanism dependent upon the CART NRSE. PMID: 18485095
the recovery of neurosecretion depends on the reciprocal level of BHC80 and REST, with BHC80 working as a negative modulator of REST repression PMID: 19439607
RE1 silencing transcription factor (REST) plays a contributory role in the neuron-specific expression of the alpha-internexin, NF-H and NF-M genes PMID: 19457133
REST-RE-1 is required for the transcriptional upregulation of Kcc2 that contributes to the shift in ontogenetic chloride concentration and GABA action in cortical neurons. PMID: 19923298

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Subcellular Location

Nucleus. Cytoplasm.; [Isoform 6]: Nucleus.

Tissue Specificity

Expressed in the hippocampus including the granule cell layer of the dentate gyrus, the pyramidal cell layers of CA1 and CA3, the apical and basilar dendrite layers of the stratum radiatum and stratum oriens of CA1, the stratum lucidum and stratum oriens

Database Links

KEGG: rno:83618

STRING: 10116.ENSRNOP00000002837

UniGene: Rn.10879

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Recombinant Rat RE1-silencing transcription factor (Rest), partial

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