Recombinant Rat Rho guanine nucleotide exchange factor 7 (Arhgef7)

Code CSB-YP002056RA
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Source Yeast
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Code CSB-EP002056RA
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Source E.coli
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Code CSB-EP002056RA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP002056RA
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Source Baculovirus
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Code CSB-MP002056RA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Arhgef7
Uniprot No.
Alternative Names
Arhgef7; Pak3bp; Pixb; Rho guanine nucleotide exchange factor 7; Beta-Pix; PAK-interacting exchange factor beta
Species
Rattus norvegicus (Rat)
Expression Region
1-646
Target Protein Sequence
MTDNANSQLV VRAKFNFQQT NEDELSFSKG DVIHVTRVEE GGWWEGTHNG RTGWFPSNYV REIKPSEKPV SPKSGTLKSP PKGFDTTAIN KSYYNVVLQN ILETEHEYSK ELQSVLSTYL WPLQTSEKLS SANTSYLMGN LEEISSFQQV LVQSLEECTK SPEAQQRVGG CFLSLMPQMR TLYLAYCANH PSAVSVLTEH SEDLGEFMET KGASSPGILV LTTGLSKPFM RLDKYPTLLK ELERHMEDYH PDRQDIQKSM TAFKNLSAQC QEVRKRKELE LQILTEPIRS WEGDDIKTLG SVTYMSQVTI QCAGSEEKNE RYLLLFPNLL LMLSASPRMS GFIYQGKLPT TGMTITKLED SENHRNAFEI SGSMIERILV SCNNQQDLHE WVEHLQRQTK VTSVSNPTIK PHSVPSHTLP SHPLTPSSKH ADSKPVALTP AYHTLPHPSH HGTPHTTISW GPLEPPKTPK PWSLSCLRPA PPLRPSAALC YKEDLSRSPK TMKKLLPKRK PERKPSDEEF AVRKSTAALE EDAQILKVIE AYCTSAKTRQ TLNSSSRKES APQVLLPEEE KIIVEETKSN GQTVIEEKSL VDTVYALKDE VQELRQDNKK MKKSLEEEQR ARKDLEKLVR KVLKNMNDPA WDETNL
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions. May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons.
Gene References into Functions
  1. Therefore, we propose that PKCgamma positively modulates dopamine release through beta2PIX phosphorylation. PMID: 25009260
  2. betaPix is not involved in the acute effect of ET-1 on channel open probability but instead mediates chronic decrease of number of active channels. PMID: 20338996
  3. has a role in bFGF-induced neurite outgrowth via the ERK cascade PMID: 12226077
  4. These results identify p66Shc and FOXO3a as novel partners of beta(1)Pix and represent the first direct evidence of beta(1)Pix in cell proliferation via Erk/p66Shc-dependent and Akt-independent mechanisms. PMID: 18385518
  5. Arhgef7-specific siRNA treatment significantly inhibited Angiotensin II -induced vascular smooth muscle cell migration. PMID: 19322025

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Subcellular Location
Cell junction, focal adhesion. Cell projection, ruffle. Cytoplasm, cell cortex. Cell projection, lamellipodium. Note=Detected at cell adhesions. A small proportion is detected at focal adhesions.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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