Recombinant Rat Serpin H1 (Serpinh1)

Code CSB-YP021087RA
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP021087RA
MSDS
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Source E.coli
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Code CSB-EP021087RA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP021087RA
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP021087RA
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Serpinh1
Uniprot No.
Alternative Names
Serpinh1; Cbp1; Hsp47; Serpin H1; 47 kDa heat shock protein; Collagen-binding protein; Colligin; GP46
Species
Rattus norvegicus (Rat)
Expression Region
18-417
Target Protein Sequence
AEV KKPVEAAAPG TAEKLSSKAT TLAERSTGLA FSLYQAMAKD QAVENILLSP LVVASSLGLV SLGGKATTAS QAKAVLSAEK LRDEEVHTGL GELVRSLSNS TARNVTWKLG SRLYGPSSVS FADDFVRSSK QHYNCEHSKI NFRDKRSALQ SINEWASQTT DGKLPEVTKD VERTDGALLV NAMFFKPHWD EKFHHKMVDN RGFMVTRSYT VGVTMMHRTG LYNYYDDEKE KLQLVEMPLA HKLSSLIILM PHHVEPLERL EKLLTKEQLK TWMGKMQKKA VAISLPKGVV EVTHDLQKHL AGLGLTEAID KNKADLSRMS GKKDLYLASV FHATAFEWDT EGNPFDQDIY GREELRSPKL FYADHPFIFL VRDNQSGSLL FIGRLVRPKG DKMRDEL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Gene References into Functions
  1. The results suggest that Hsp47 turnover may increase during heat stress or that Hsp47 consumption exceeds its production. PMID: 25526199
  2. miR-29b down-regulates heat shock protein 47 and LOX expression. PMID: 24650661
  3. HSP47 specifically modulates TGF-beta1-induced extracellular matrix protein synthesis in HK-2 cells PMID: 22718885
  4. Heat shock protein 47 (HSP47) antisense oligonucleotides reduce cardiac remodeling and improve cardiac function in a rat model of myocardial infarction. PMID: 21412710
  5. Involvement of HSP47 in progression of peritoneal fibrosis. Inhibition of HSP47 expression might merit further clinical investigation for treatment of peritoneal fibrosis in continuous ambulatory peritoneal dialysis. PMID: 12911538
  6. Microgravity differentially modulated the expression of the molecular chaperone HSP47 in osteoblasts PMID: 15033773
  7. expression of HSP47, a collagen-specific molecular chaperone, responds to gravitational changes, including microgravity and hypergravity in vitro and in vivo PMID: 17054723

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Subcellular Location
Endoplasmic reticulum lumen.
Protein Families
Serpin family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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