Recombinant Rat Sodium channel protein type 5 subunit alpha (Scn5a), partial

Code CSB-YP020843RA
MSDS
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Source Yeast
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Code CSB-EP020843RA
MSDS
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Source E.coli
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Code CSB-EP020843RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP020843RA
MSDS
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Source Baculovirus
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Code CSB-MP020843RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Scn5a
Uniprot No.
Alternative Names
Scn5a; Sodium channel protein type 5 subunit alpha; Sodium channel protein cardiac muscle subunit alpha; Sodium channel protein type V subunit alpha; Voltage-gated sodium channel subunit alpha Nav1.5
Species
Rattus norvegicus (Rat)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient. It is a tetrodotoxin-resistant Na(+) channel isoform. This channel is responsible for the initial upstroke of the action potential. Channel inactivation is regulated by intracellular calcium levels.
Gene References into Functions
  1. The failing heart has ~30% lower SCN5A levels. PMID: 28205593
  2. The upregulation of PKC-gamma may directly or indirectly downregulate the expression of Nav1.5 isoforms in spared nerve injury rat models, which may further involve in the pathological process of neuropathic pain. PMID: 29393394
  3. Clinically relevant cardiotoxicity of domperidone and metoclopramide corresponds to a rather potent and local anesthetic-like inhibition of cardiac Na channels including Nav1.5. PMID: 27861438
  4. Nav1.5 N-terminal domain binding to alpha1-syntrophin increases membrane density of human Kir2.1, Kir2.2 and Nav1.5 channels PMID: 26786162
  5. Scn5a is expressed in jejunum and colon smooth muscle and it contributes to smooth muscle electrophysiology. PMID: 26459913
  6. UBR3/6 regulate cardiomyocyte Nav 1.5 channel protein levels via the ubiquitin-proteasome pathway. PMID: 26059563
  7. Flecainide (1 mum) rescues the RyR2-P2328S atrial arrhythmogenic phenotype by restoring compromised INa and lambda, changes recently attributed to increased sarcoplasmic reticular Ca(2+) release. PMID: 25850710
  8. As in the brain, epilepsy was associated with an enhanced expression of the neuronal isoform NaV1.1 in cardiomyocyte. Epilepsy was also associated with a lower INa activation threshold resulting in increased cell excitability. PMID: 26067667
  9. Provocation of an autoimmune response against NaV1.5 induces cardiac conduction defects. PMID: 23684688
  10. The voltage gated sodium channel Na(v)1.5, which is responsible for initiation of the action potential, was found to interact with perinexal Cx43 but not with ZO-1. PMID: 22811280
  11. Nav1.5 (rN1) has a newly identified exon for alternative splicing that is differentfrom rat heart and is more widely expressed in rat brain than previously thought. PMID: 22331407
  12. Na(v)1.5-dependent increase in Na(+influx activates CaMKII, which in turn phosphorylates Na(v)1.5, further promoting Na(+) influx. Pharmacological inhibition of either CaMKII or Na(v)1.5 ameliorates cardiac dysfunction caused by excessive Na(+) influx. PMID: 21677263
  13. These results suggest that Nav1.5 channels in human and rat brains are encoded by new variants of Nav1.5/SCN5A and Nav1.5 is more widely distributed and expressed than previously thought. PMID: 19376164
  14. Altering the oxidized to reduced NAD(H) balance can activate mitochondrial reactive oxygen species production, leading to reduced I(Na) via SCN5A PMID: 20724705
  15. In cultured adult rat skeletal muscle fibers, an increase of the Na(V)1.5 channel type was observed. These channels exhibited increased activation time constant (tau(m)) and reduced conductance. PMID: 20517693
  16. novel isoforms of Na(v)1.8 and Na(v)1.5 are each generated by alternative splicing at CAG/CAG motifs, which result in the absence or presence of predicted glutamine residues within the interdomain cytoplasmic loop IDII/III PMID: 15047701
  17. Nav1.5 is expressed in gastric epithelial cells and function as growth modulators. PMID: 16331678
  18. Na(V)1.5 was abundant in the atrial & ventricular myocardium & the left bundle branch, but absent in the open node, penetrating AV bundle, AV ring bundle, & common bundle. A reduced level was found in the inferior nodal extension & transitional zone. PMID: 16966585
  19. Skeletal myotubes adopt a cardiac-like phenotype in cell culture conditions and that the expression of Na(v)1.5 acts as an underlying molecular mechanism. PMID: 17900547
  20. ANG II can decrease scn5a transcription and current. This effect appears to be through production of H(2)O(2) resulting in NF-kappaB binding to the Na(+) channel promoter. PMID: 18032528
  21. PKP2 associates with Na(V)1.5, and that knockdown of PKP2 expression alters the properties of the sodium current, and the velocity of action potential propagation in cultured cardiomyocytes. PMID: 19661460
  22. Mutations of GPD1-L may downregulate Na(v)1.5 by altering the oxidized to reduced NAD(H) balance. PMID: 19745168

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Cytoplasm, perinuclear region. Cell membrane, sarcolemma, T-tubule.
Protein Families
Sodium channel (TC 1.A.1.10) family, Nav1.5/SCN5A subfamily
Tissue Specificity
Strongly expressed in the heart. Also expressed in adult and fetal brain, spinal cord, testis, and at moderate levels in kidney, adrenal gland, lung, skeletal muscle, spleen, stomach and bladder. Isoform 2 is expressed in brain.
Database Links
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