Recombinant Rat Somatostatin (Sst)

Code CSB-YP022723RA
MSDS
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Source Yeast
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Code CSB-EP022723RA
MSDS
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Source E.coli
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Code CSB-EP022723RA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP022723RA
MSDS
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Source Baculovirus
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Code CSB-MP022723RA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Sst
Uniprot No.
Alternative Names
Sst; Smst; Somatostatin; somatotropin release-inhibiting factor; SRIF) [Cleaved into: Antrin; Somatostatin-28; Somatostatin-14; Neuronostatin; NST)]
Species
Rattus norvegicus (Rat)
Protein Length
Cytoplasmic domain
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Inhibits the secretion of pituitary hormones, including that of growth hormone/somatotropin (GH1), PRL, ACTH, luteinizing hormone (LH) and TSH. Also impairs ghrelin- and GnRH-stimulated secretion of GH1 and LH; the inhibition of ghrelin-stimulated secretion of GH1 can be further increased by neuronostatin.; May enhance low-glucose-induced glucagon release by pancreatic alpha cells. This effect may be mediated by binding to GPR107 and PKA activation. May regulate cardiac contractile function. May compromise cardiomyocyte viability. In the central nervous system, may impair memory retention and may affect hippocampal excitability. May also have anxiolytic and anorexigenic effects. May play a role in arterial pressure regulation. May inhibit basal, but not ghrelin- or GnRH-stimulated secretion of GH1 or LH, but does not affect the release of other pituitary hormones, including PRL, ACTH, FSH or TSH. Potentiates inhibitory action of somatostatin on ghrelin-stimulated secretion of GH1, but not that on GnRH-stimulated secretion of LH.
Gene References into Functions
  1. Results suggest that somatostatin suppresses GABA release from the amacrine cells by inhibiting presynaptic Ca2+ channels, in part through activating sst5/sst2 receptors, a process that is mediated by the intracellular cAMP-PKA signaling pathway PMID: 24846611
  2. These results suggest that a local release of somatostatin facilitates the excitability of trigeminal nocicepitve C1 neuronal activity via inhibition of GABAergic neurons PMID: 24321530
  3. when activated by SST-14, SSTR2A internalizes and recycles via the Golgi, which requires ECE-1 degradation of SST-14 and receptor dissociation from beta-arrestins PMID: 23913690
  4. Helicobacter pylori is capable of inhibiting acid secretion directly as well as indirectly by activating intramural CGRP sensory neurons coupled to stimulation of SST and inhibition of histamine secretion. PMID: 23392237
  5. Data suggest that neuronostatin action to reduce food intake and water intake is mediated through melanocortin 3/4 receptors, not through oxytocin receptors. PMID: 20600426
  6. both ferret odor exposure and elevated plus maze exposure resulted in increased somatostatin PMID: 22917777
  7. This study provides structural evidence that SST immunoreactivity in the pre-BotC is highly expressed in somas, terminals, and primary dendrites, and overlapped with small fusiform NK1R-ir neurons. PMID: 22765158
  8. Surviving somatostatin interneurons display increased mRNA levels at late intervals (3 months) after kainic acid-induced epilepsy. PMID: 22437342
  9. Neuronostatin, a novel peptide encoded by somatostatin gene, regulates cardiac contractile function and cardiomyocyte survival. PMID: 22170057
  10. These findings suggest that somatostatin directly inhibits the activity of rat GnRH neurons through volume transmission in the organum vasculosum of the lamina terminalis region. PMID: 22147011
  11. 5-HT(3) receptor-mediated somatostatin-dependent secretoinhibitory pathway is suppressed in the water-immersion restraint stressed rats PMID: 21291881
  12. Pancreatic islet CCK1/2 receptors initiate somatosatin secretion through constitutive secretion controlled at the level of protein synthesis. PMID: 19959964
  13. pancreatic release of somatostatin inhibited by ghrelin PMID: 11834435
  14. data suggest that methionine enkephalin, neurotensin and somatostatin are released in the caudal trigeminal nucleus after electrical stimulation of the trigeminal ganglion, an experimental model of migraine PMID: 12080490
  15. High temperature can affect gastrin, somatostatin and motilin production in the gastric antral mucosa of rats with gastric ulcer. PMID: 12376287
  16. The remarkable increase in somatostatin immunoreactivity in the aged rat suprachiasmatic nucleus reveals that not all suprachiasmatic neuropeptidergic systems decline with age, and shows an age-related role for somatostatin in the suprachiasmatic nucleus. PMID: 12470886
  17. Data suggest that distention of isolated rat stomach stimulates somatostatin release via cholinergic and non-cholinergic TTX-insensitive pathways, and that these pathways are responsible for distention-induced inhibition of gastrin release. PMID: 12508367
  18. antral atrial natriuretic peptide (ANP) and somatostatin (SST) secretion linked by paracrine feedback pathways: endogenous ANP acting via the NPR-A receptor stimulates SST secretion; endogenous SST acting via the sst2 receptor inhibits ANP secretion PMID: 12527142
  19. adrenomedullin, acting via intramural fundic neurons, stimulates somatostatin and thus inhibits histamine and acid secretion PMID: 12573799
  20. Presence and cellular localization of somatostatin in the three lipid-containing lacrimal glands of the rat. PMID: 12613892
  21. ghrelin secretion from the stomach is regulated by gastric somatostatin PMID: 12615065
  22. fasting for 48 hours increases somatostatin synthesis and receptor subtype expression PMID: 12832106
  23. Somatostatin is involved in the transcriptional regulation of the GH gene, and that the effect of SRIF is mediated through pertussis toxin-sensitive G protein PMID: 14664705
  24. L-glutamate triggers somatostatin secretion from delta-cells by way of the GluR4c-flip receptor under low-glucose conditions. The released somatostatin may complete the feedback inhibition of alpha-cells. PMID: 15220198
  25. A second pathway is required to induce changes in srif and sst2 expression, since constitutively active calcineurin alone is not sufficient to induce these changes. PMID: 16000155
  26. Somatostatin-immunoreactive terminals particularly targeted intrinsic cardiac neurons that were immunoreactive for calbindin PMID: 16374620
  27. These findings suggest that inhibitory inputs from SOM+ interneurons may interact with excitatory inputs to pyramidal cell distal dendrites in the BLa. These interactions might affect synaptic plasticity related to emotional learning. PMID: 17120289
  28. only bistratified cells express the neuropeptide somatostatin PMID: 17122364
  29. Sex-specific, transient transcription of the somatostatin gene may causally relate to the estrogen-dependent organization of the Sexually Dimorphic Nucleus-Preoptic area. PMID: 17138650
  30. The adrenergic system is not involved in producing HISS-dependent insulin resistance in response to hemorrhage. Somatostatin appears to be the hormonal regulator of this response; that the somatostatin derives from a neural origin within the liver PMID: 17202832
  31. RT-PCR and immunocytochemistry studies support the presence of somatostatin in rat Kupffer cells. PMID: 17481746
  32. our data anticipate the possibility that carboxypeptidase-E, prohormone convertase 1, and prohormone convertase 2 might be potential target for the maturation of somatostatin PMID: 17543468
  33. Somatostatin and glucagon pulses were coupled in antisynchronous fashion, supporting the idea that the delta-cells have a local inhibitory effect on glucagon release. PMID: 17628719
  34. Present results strongly support the neuroinflammation as a potential factor involved in the age-related degeneration of somatostatin GABAergic cells. PMID: 17666053
  35. SS-14 is not involved in the control of the physiologic involution of the thymus, though it induces thymic weight loss through the reduction of true lymphoid tissue; SS-14 modulates thymocytes development and maturation PMID: 17761280
  36. Endogenously released somatostatin may induce slow inhibitory postsynaptic currents through the activation of GIRK channels in substantia gelatinosa neurons; this slow synaptic transmission might play an important role in spinal antinociception. PMID: 18356203
  37. Taken together, these results provide first evidence that the presence of SST is a unique feature for the selective sparing of medium sized aspiny interneurons in excitotoxicity. PMID: 18483877
  38. Data show that SOM neurons in the claustrum do not reveal any significant changes in their numerical density during the postnatal period. PMID: 18598846
  39. Inhibitory effect of Sst on prostaglandin E2 synthesis by primary neonatal rat glial cells is reported. PMID: 18619496
  40. SST-14 was cointernalized with SSTR3, was uncoupled from receptor, & was sorted into an endocytic degradation pathway, whereas octreotide was recycled as an intact peptide. Chronic stimulation of SSTR3 induced time-dependent downregulation of receptor. PMID: 18702662
  41. Somatostatin secretion in perfused rat pancreas by obestatin PMID: 18773927
  42. Release of regulatory gut peptides somatostatin, by acid and hyperosmolal solutions in the intestine in conscious rats PMID: 18992283
  43. These data support involvement of SRIF2B (SSTR4) receptors in the negative contractile effects of SRIF-14, while one or more of the three SRIF1 receptor subtypes (SSTR2, 3 or 5) may contribute to the positive contractile effects of SRIF-14. PMID: 19088447
  44. Somatostatin has no effect on basal and IL-1beta-stimulated PGE(2) release, whereas cortistatin reduced baseline prostanoids production and abolished stimulation elicited by IL-1beta. PMID: 19552966
  45. These results provide evidence that somatostatin tonically inhibits the peripheral nerve terminals of small-diameter cutaneous afferent fibers. PMID: 19596279

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Subcellular Location
Secreted.
Protein Families
Somatostatin family
Tissue Specificity
Somatostatin is detected in neurons throughout the brain, including in the lateral septum, nucleus accumbens, amygdaloid complex, hypothalamic periventricular nucleus, hippocampus, cortex, cerebellum and several brainstem nuclei (at protein level). Neuron
Database Links
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