Recombinant Replication protein E1(E1) ,partial

Code CSB-YP322157HNS
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Source Yeast
Code CSB-EP322157HNS
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Source E.coli
Code CSB-EP322157HNS-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Code CSB-BP322157HNS
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Source Baculovirus
Code CSB-MP322157HNS
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Source Mammalian cell

Product Details

Purity >85% (SDS-PAGE)
Target Names E1
Uniprot No. P22419
Species Human papillomavirus type 47
Expression Region 1-605
Target Protein Sequence MADSKGSTSK EGFGDWCILE ADCSDVEDDL GQLFERDTDS DISDLLDNCD LDQGNSRELF HQQECKQSEE QLQKLKRKYL SPKAVAQLSP RLESISLSPQ QKSKRRLFAE QDSGLELTFN NEAEDVTPEV EVPAIDSRPD DDEGGSGDVD IHYTALLRSS NQKATLLAKF KQAFGVGFNE LTRQFKSYKT CCNHWVVSVY AVHDDLFESS KQLLQQHCDY IWVRGIDAMS LYLLCFKAGK NRGTVHKLIT TMLNVHEQQI LSEPPKLRNT AAALFWYKGC MGPGVFTHGP YPEWIAQLTI LGHKSAEASA FDLSVMVQWA FDNNLFEEAD IAYGYARLAP EDSNAVAWLA HNNQAKYVRE CAMMVRYYKK GQMRDMSMSE WIYTRIHEVE GEGQWSSIVK FLRYQEINFI SFLAALKDLL HSVPKRNCIL FHGPPNTGKS SFGMSLIKVL RGRVLSFVNS KSQFWLQPLG ECKIALLDDV TDPCWVYMDQ YLRNGLDGHF VSLDCKYRAP MQTKFPPLIL TSNINVHAET NYRYLHSRIK GFEFKNPFPM KADNTPQFEL TDQSWKSFFT RLWTHLDLSD QEDEGEHGES QRAFQCSART ANEHL
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.
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Target Data

Function ATP-dependent DNA helicase required for initiation of viral DNA replication. It forms a complex with the viral E2 protein. The E1-E2 complex binds to the replication origin which contains binding sites for both proteins. During the initial step, a dimer of E1 interacts with a dimer of protein E2 leading to a complex that binds the viral origin of replication with high specificity. Then, a second dimer of E1 displaces the E2 dimer in an ATP-dependent manner to form the E1 tetramer. Following this, two E1 monomers are added to each half of the site, which results in the formation of two E1 trimers on the viral ori. Subsequently, two hexamers will be created. The double hexamer acts as a bi-directional helicase machinery and unwinds the viral DNA and then recruits the host DNA polymerase to start replication.
Subcellular Location Host nucleus
Protein Families Papillomaviridae E1 protein family

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