Recombinant Rhodobacter capsulatus FeMo cofactor biosynthesis protein nifB (nifB)

Code CSB-YP322363RLC
MSDS
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Source Yeast
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Code CSB-EP322363RLC
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Source E.coli
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Code CSB-EP322363RLC-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP322363RLC
MSDS
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Source Baculovirus
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Code CSB-MP322363RLC
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
nifB
Uniprot No.
Alternative Names
nifB; FeMo cofactor biosynthesis protein NifB; EC 4.-.-.-; Nitrogenase cofactor maturase NifB; Radical SAM assemblase NifB
Species
Rhodobacter capsulatus (Rhodopseudomonas capsulata)
Expression Region
1-452
Target Protein Sequence
MDPATWAKVK DHPCYSEEAH HHFARMHVSV APACNIQCNY CNRKYDCSNE SRPGVVSERL TPEEAARKVI AVANEVPQLS VLGIAGPGDS AYDWLKTKET FRLVTAQIPD IKLCLSSNGL ALPDHLDELV EMNVDHVTIT INMIDPEVGA KIYPWIFYNR KRIYGVEASR ILHERQMAAL DGLVARGILV KVNSVLIPGI NDAHLLEVNR EVKRRGAFLH NIMPLISAPE HGTHFGLTGQ RGPTAAELLA VQEACAGGAN LMKHCRQCRA DAVGLLGEDR GQEFTLDLVP EAPVYDPAAR ETYRDWVGAE REDRRAAAEA AQAATEAACA ASSPKLLVAV TTQGGGRINQ HFGHATEFQV FEVDATGVRF AFHRRCDNYC VDGGGAEDRL DRVIAALDGI DTVLVAKIGD CPREGLASAG ITARDSWAHD YIEPAILALY RERMTQKQAI TA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the biosynthesis of the iron-molybdenum cofactor (FeMo-co or M-cluster) found in the dinitrogenase enzyme of the nitrogenase complex in nitrogen-fixing microorganisms. NifB catalyzes the crucial step of radical SAM-dependent carbide insertion that occurs concomitant with the insertion of a 9th sulfur and the rearrangement/coupling of two [4Fe-4S] clusters into a [8Fe-9S-C] cluster, the precursor to the M-cluster.
Protein Families
Radical SAM superfamily, NifB family
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