Recombinant Rhodobacter sphaeroides Queuine tRNA-ribosyltransferase(tgt)

Code CSB-YP019146RIJ
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Source Yeast
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Code CSB-EP019146RIJ
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Source E.coli
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Code CSB-EP019146RIJ-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP019146RIJ
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Source Baculovirus
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Code CSB-MP019146RIJ
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names tgt
Uniprot No. A3PJT9
Alternative Names tgt; Rsph17029_1495; Queuine tRNA-ribosyltransferase; EC 2.4.2.29; Guanine insertion enzyme; tRNA-guanine transglycosylase
Species Rhodobacter sphaeroides (strain ATCC 17029 / ATH 2.4.9)
Expression Region 1-376
Target Protein Sequence MTQRFSFELT ATDGRARTGV ISTPRGEIRT PAFMPVGTAG TVKAMLPENV RATGADILLG NTYHLMLRPT AERVARLGGL HRFMNWDRPI LTDSGGFQVM SLADLRKLSE EGVTFRSHID GSKHHLSPER SMEIQRLLGS DIVMAFDECP ALPATEEAVA QSMRLSMRWA RRSREAFGDR PGHALFGIMQ GGVTRDLREE SAAALREIGF EGYAIGGLAV GEGQEAMFGV LDYAPGFLPE DRPRYLMGVG KPDDIVGAVE RGVDMMDCVL PSRSGRTGQA WTRRGQVNIK NARHMDDPRP LDEACSCPAC RSYSRAYLHH VFRAQEIIAS MLLTWHNLHY YQELMQGLRT AIAAGRLGEF VAAFHAARAE GDIEPL
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Protein Families Queuine tRNA-ribosyltransferase family
Database Links

KEGG: rsh:Rsph17029_1495

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