Recombinant Ribosomal RNA large subunit methyltransferase N(rlmN)

Product Type Recombinant Protein
Uniprot No. A4G4J9
Species Herminiimonas arsenicoxydans
Gene Names rlmN
Protein Names Recommended name: Ribosomal RNA large subunit methyltransferase N EC= 2.1.1.192 Alternative name(s): 23S rRNA (adenine(2503)-C(2))-methyltransferase 23S rRNA m2A2503 methyltransferase
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Specifically methylates position 2 of adenine 2503 in 23S rRNA and position 2 of adenine 37 in tRNAs. m2A2503 modification seems to play a crucial role in the proofreading step occurring at the peptidyl transferase center and thus would serve to optimize ribosomal fidelity.
Subcellular Location Cytoplasm
Protein Families Radical SAM superfamily, RlmN family
Database Links

KEGG: har:HEAR1261

STRING: 204773.HEAR1261

Product Details for Different Expression Systems

Product Name Recombinant Ribosomal RNA large subunit methyltransferase N(rlmN),Yeast
Code CSB-YP389998HSJ
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MTTTLTNLLD LDPAQLIAYC GELGEKPFRA KQLQRWIHQF GASDFDAMTD LAKSLRDKLK TRAMIAAPAV ISDHTSSDGT RKWLIDVGQG NAVETVFIPE ENRGTLCIST QAGCAVNCRF CSTGKQGFNR NLSVGEIIGQ LWMAEFELRR TKGIEPGPKG ERQITNVVMM GMGEPLLNYE PTVTALKLML DDNAYGLSRR RVTLSTSGVV PMIDKLSQDC AVALAVSLHA SNDALRDGLV PLNKKYPLQE LMAACKRYLE FAPRDFVTFE YCMLDGVNDS DQHARELLTL VRDVPCKFNL IPFNPFPESG LTRSNNPRIK AFAQVLMDGG LVTTIRKTRG DDIDAACGQL AGEVQDRTRV QDRMKKMAEY QEKFGKNFGR IVEIPS
Expression Region 1-386
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Ribosomal RNA large subunit methyltransferase N(rlmN),E.coli
Code CSB-EP389998HSJ
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MTTTLTNLLD LDPAQLIAYC GELGEKPFRA KQLQRWIHQF GASDFDAMTD LAKSLRDKLK TRAMIAAPAV ISDHTSSDGT RKWLIDVGQG NAVETVFIPE ENRGTLCIST QAGCAVNCRF CSTGKQGFNR NLSVGEIIGQ LWMAEFELRR TKGIEPGPKG ERQITNVVMM GMGEPLLNYE PTVTALKLML DDNAYGLSRR RVTLSTSGVV PMIDKLSQDC AVALAVSLHA SNDALRDGLV PLNKKYPLQE LMAACKRYLE FAPRDFVTFE YCMLDGVNDS DQHARELLTL VRDVPCKFNL IPFNPFPESG LTRSNNPRIK AFAQVLMDGG LVTTIRKTRG DDIDAACGQL AGEVQDRTRV QDRMKKMAEY QEKFGKNFGR IVEIPS
Expression Region 1-386
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Ribosomal RNA large subunit methyltransferase N(rlmN),Baculovirus
Code CSB-BP389998HSJ
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MTTTLTNLLD LDPAQLIAYC GELGEKPFRA KQLQRWIHQF GASDFDAMTD LAKSLRDKLK TRAMIAAPAV ISDHTSSDGT RKWLIDVGQG NAVETVFIPE ENRGTLCIST QAGCAVNCRF CSTGKQGFNR NLSVGEIIGQ LWMAEFELRR TKGIEPGPKG ERQITNVVMM GMGEPLLNYE PTVTALKLML DDNAYGLSRR RVTLSTSGVV PMIDKLSQDC AVALAVSLHA SNDALRDGLV PLNKKYPLQE LMAACKRYLE FAPRDFVTFE YCMLDGVNDS DQHARELLTL VRDVPCKFNL IPFNPFPESG LTRSNNPRIK AFAQVLMDGG LVTTIRKTRG DDIDAACGQL AGEVQDRTRV QDRMKKMAEY QEKFGKNFGR IVEIPS
Expression Region 1-386
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Ribosomal RNA large subunit methyltransferase N(rlmN),Mammalian cell
Code CSB-MP389998HSJ
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MTTTLTNLLD LDPAQLIAYC GELGEKPFRA KQLQRWIHQF GASDFDAMTD LAKSLRDKLK TRAMIAAPAV ISDHTSSDGT RKWLIDVGQG NAVETVFIPE ENRGTLCIST QAGCAVNCRF CSTGKQGFNR NLSVGEIIGQ LWMAEFELRR TKGIEPGPKG ERQITNVVMM GMGEPLLNYE PTVTALKLML DDNAYGLSRR RVTLSTSGVV PMIDKLSQDC AVALAVSLHA SNDALRDGLV PLNKKYPLQE LMAACKRYLE FAPRDFVTFE YCMLDGVNDS DQHARELLTL VRDVPCKFNL IPFNPFPESG LTRSNNPRIK AFAQVLMDGG LVTTIRKTRG DDIDAACGQL AGEVQDRTRV QDRMKKMAEY QEKFGKNFGR IVEIPS
Expression Region 1-386
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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