Recombinant Rickettsia bellii Queuine tRNA-ribosyltransferase(tgt)

Product Type Recombinant Protein
Uniprot No. Q1RH25
Abbreviation tgt
Species Rickettsia bellii (strain RML369-C)
Gene Names tgt
Protein Names Recommended name: Queuine tRNA-ribosyltransferase EC= 2.4.2.29 Alternative name(s): Guanine insertion enzyme tRNA-guanine transglycosylase
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Catalyzes the base-exchange of a guanine (G) residue with the queuine precursor 7-aminomethyl-7-deazaguanine (PreQ1) at position 34 (anticodon wobble position) in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). Catalysis occurs through a double-displacement mechanism. The nucleophile active site attacks the C1' of nucleotide 34 to detach the guanine base from the RNA, forming a covalent enzyme-RNA intermediate. The proton acceptor active site deprotonates the incoming PreQ1, allowing a nucleophilic attack on the C1' of the ribose to form the product. After dissociation, two additional enzymatic reactions on the tRNA convert PreQ1 to queuine (Q), resulting in the hypermodified nucleoside queuosine (7-(((4,5-cis-dihydroxy-2-cyclopenten-1-yl)amino)methyl)-7-deazaguanosine).
Protein Families Queuine tRNA-ribosyltransferase family
Database Links

KEGG: rbe:RBE_1258

Product Details for Different Expression Systems

Product Name Recombinant Rickettsia bellii Queuine tRNA-ribosyltransferase(tgt),Yeast
Code CSB-YP630147RAAI
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MSKFSFNINH QYKKARSGII TTAHGNIRTP AFMPVGTRGT VKAMLPESVA ETGADILLGN TYHLMLQPSA ERIAKLGGLH KFMNWGKPIL TDSGGFQVMS LSKLRKITEE GVSFNSHING DKYLLTPERS TEIQHLLGST ITMAFDECTP YPATFEEAKT SMQLTTRWAH RSREAFVKRD GYAQFGIIQG STYEELREQS AKDLIELDFE GYAIGGLAVG EGQELMFKVL APDFLPQNKP RYLMGVGKPA DIIGAVSRGV DMFDCVIPTR SGRNGQAFTK YGTVNIRNSK YAEDNDPLEA DCLCPACQNY SKAYLHHLVR IGEILGAMLM TWHNLTYFQN LMSRIREYIA LGKDFDFTT
Expression Region 1-359
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Rickettsia bellii Queuine tRNA-ribosyltransferase(tgt),E.coli
Code CSB-EP630147RAAI
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MSKFSFNINH QYKKARSGII TTAHGNIRTP AFMPVGTRGT VKAMLPESVA ETGADILLGN TYHLMLQPSA ERIAKLGGLH KFMNWGKPIL TDSGGFQVMS LSKLRKITEE GVSFNSHING DKYLLTPERS TEIQHLLGST ITMAFDECTP YPATFEEAKT SMQLTTRWAH RSREAFVKRD GYAQFGIIQG STYEELREQS AKDLIELDFE GYAIGGLAVG EGQELMFKVL APDFLPQNKP RYLMGVGKPA DIIGAVSRGV DMFDCVIPTR SGRNGQAFTK YGTVNIRNSK YAEDNDPLEA DCLCPACQNY SKAYLHHLVR IGEILGAMLM TWHNLTYFQN LMSRIREYIA LGKDFDFTT
Expression Region 1-359
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Rickettsia bellii Queuine tRNA-ribosyltransferase(tgt),Baculovirus
Code CSB-BP630147RAAI
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MSKFSFNINH QYKKARSGII TTAHGNIRTP AFMPVGTRGT VKAMLPESVA ETGADILLGN TYHLMLQPSA ERIAKLGGLH KFMNWGKPIL TDSGGFQVMS LSKLRKITEE GVSFNSHING DKYLLTPERS TEIQHLLGST ITMAFDECTP YPATFEEAKT SMQLTTRWAH RSREAFVKRD GYAQFGIIQG STYEELREQS AKDLIELDFE GYAIGGLAVG EGQELMFKVL APDFLPQNKP RYLMGVGKPA DIIGAVSRGV DMFDCVIPTR SGRNGQAFTK YGTVNIRNSK YAEDNDPLEA DCLCPACQNY SKAYLHHLVR IGEILGAMLM TWHNLTYFQN LMSRIREYIA LGKDFDFTT
Expression Region 1-359
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Rickettsia bellii Queuine tRNA-ribosyltransferase(tgt),Mammalian cell
Code CSB-MP630147RAAI
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MSKFSFNINH QYKKARSGII TTAHGNIRTP AFMPVGTRGT VKAMLPESVA ETGADILLGN TYHLMLQPSA ERIAKLGGLH KFMNWGKPIL TDSGGFQVMS LSKLRKITEE GVSFNSHING DKYLLTPERS TEIQHLLGST ITMAFDECTP YPATFEEAKT SMQLTTRWAH RSREAFVKRD GYAQFGIIQG STYEELREQS AKDLIELDFE GYAIGGLAVG EGQELMFKVL APDFLPQNKP RYLMGVGKPA DIIGAVSRGV DMFDCVIPTR SGRNGQAFTK YGTVNIRNSK YAEDNDPLEA DCLCPACQNY SKAYLHHLVR IGEILGAMLM TWHNLTYFQN LMSRIREYIA LGKDFDFTT
Expression Region 1-359
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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