Recombinant Saccharomonospora viridis Pup--protein ligase(pafA)

Product Type Recombinant Protein
Uniprot No. C7MWV3
Species Saccharomonospora viridis (strain ATCC 15386 / DSM 43017 / JCM 3036 / NBRC 12207 / P101)
Gene Names pafA
Protein Names Recommended name: Pup--protein ligase EC= 6.3.2.n2 Alternative name(s): Proteasome accessory factor A Pup-conjugating enzyme
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Function Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine.
Protein Families Pup ligase/Pup deamidase family, Pup-conjugating enzyme subfamily
Database Links

KEGG: svi:Svir_21990

STRING: 471857.Svir_21990

Product Details for Different Expression Systems

Product Name Recombinant Saccharomonospora viridis Pup--protein ligase(pafA),Yeast
Code CSB-YP512063SVE
Size Pls inquiry other sizes
Source Yeast
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MQRRIFGIET EFGVTCTFHG QRRLSPDEVA RYLFRRVVSW GRSSNVFLSN GSRLYLDVGS HPEYATAECD DLAQLVTHDK AGERILEDLL IDAERRLAEE GIGGDIFLFK NNTDSAGNSY GCHENYLVTR AGEFSRVADV LLPFLVTRQL VCGAGKVLQT PRGGVYCLSQ RAEHIWEGVS SATTRSRPII NTRDEPHADA ERYRRLHVIV GDSNMAEPTT LLKVGSVHLV LQMIEEGVQF RDFTLDNPIR AIREISHDLT GRRQVRLAGG REASALEIQR EYYARAVQHV ESGDPSPTTQ YLIDLWGRAL DAVEQQDFSS IDTEIDWAIK HRLVERYRSK HNLTLSDPRV AQLDLAYHDI RRGRGVFDLL QRKGMVRRIT DDGEIELAKD SPPQTTRAKL RGDFIAAAQE AGRDFTVDWV HLKLNDQAQR TVLCKDPFRS VDERVERLIN SL
Expression Region 1-452
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
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Product Name Recombinant Saccharomonospora viridis Pup--protein ligase(pafA),E.coli
Code CSB-EP512063SVE
Size Pls inquiry other sizes
Source E.coli
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-SUMO-tagged and C-terminal Myc-tagged
N-terminal His-B2M-tagged and C-terminal Myc-tagged
N-terminal His-GST-tagged and C-terminal Myc-tagged
N-terminal His-tagged
N-terminal His-SUMO-tagged
N-terminal His-B2M-tagged
N-terminal His-GST-tagged
N-terminal GST-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MQRRIFGIET EFGVTCTFHG QRRLSPDEVA RYLFRRVVSW GRSSNVFLSN GSRLYLDVGS HPEYATAECD DLAQLVTHDK AGERILEDLL IDAERRLAEE GIGGDIFLFK NNTDSAGNSY GCHENYLVTR AGEFSRVADV LLPFLVTRQL VCGAGKVLQT PRGGVYCLSQ RAEHIWEGVS SATTRSRPII NTRDEPHADA ERYRRLHVIV GDSNMAEPTT LLKVGSVHLV LQMIEEGVQF RDFTLDNPIR AIREISHDLT GRRQVRLAGG REASALEIQR EYYARAVQHV ESGDPSPTTQ YLIDLWGRAL DAVEQQDFSS IDTEIDWAIK HRLVERYRSK HNLTLSDPRV AQLDLAYHDI RRGRGVFDLL QRKGMVRRIT DDGEIELAKD SPPQTTRAKL RGDFIAAAQE AGRDFTVDWV HLKLNDQAQR TVLCKDPFRS VDERVERLIN SL
Expression Region 1-452
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Saccharomonospora viridis Pup--protein ligase(pafA),Baculovirus
Code CSB-BP512063SVE
Size Pls inquiry other sizes
Source Baculovirus
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MQRRIFGIET EFGVTCTFHG QRRLSPDEVA RYLFRRVVSW GRSSNVFLSN GSRLYLDVGS HPEYATAECD DLAQLVTHDK AGERILEDLL IDAERRLAEE GIGGDIFLFK NNTDSAGNSY GCHENYLVTR AGEFSRVADV LLPFLVTRQL VCGAGKVLQT PRGGVYCLSQ RAEHIWEGVS SATTRSRPII NTRDEPHADA ERYRRLHVIV GDSNMAEPTT LLKVGSVHLV LQMIEEGVQF RDFTLDNPIR AIREISHDLT GRRQVRLAGG REASALEIQR EYYARAVQHV ESGDPSPTTQ YLIDLWGRAL DAVEQQDFSS IDTEIDWAIK HRLVERYRSK HNLTLSDPRV AQLDLAYHDI RRGRGVFDLL QRKGMVRRIT DDGEIELAKD SPPQTTRAKL RGDFIAAAQE AGRDFTVDWV HLKLNDQAQR TVLCKDPFRS VDERVERLIN SL
Expression Region 1-452
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat
Product Name Recombinant Saccharomonospora viridis Pup--protein ligase(pafA),Mammalian cell
Code CSB-MP512063SVE
Size Pls inquiry other sizes
Source Mammalian cell
Purity >85% (SDS-PAGE)
Tag Info The following tags are available.
N-terminal His-tagged and C-terminal Myc-tagged
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Sequence MQRRIFGIET EFGVTCTFHG QRRLSPDEVA RYLFRRVVSW GRSSNVFLSN GSRLYLDVGS HPEYATAECD DLAQLVTHDK AGERILEDLL IDAERRLAEE GIGGDIFLFK NNTDSAGNSY GCHENYLVTR AGEFSRVADV LLPFLVTRQL VCGAGKVLQT PRGGVYCLSQ RAEHIWEGVS SATTRSRPII NTRDEPHADA ERYRRLHVIV GDSNMAEPTT LLKVGSVHLV LQMIEEGVQF RDFTLDNPIR AIREISHDLT GRRQVRLAGG REASALEIQR EYYARAVQHV ESGDPSPTTQ YLIDLWGRAL DAVEQQDFSS IDTEIDWAIK HRLVERYRSK HNLTLSDPRV AQLDLAYHDI RRGRGVFDLL QRKGMVRRIT DDGEIELAKD SPPQTTRAKL RGDFIAAAQE AGRDFTVDWV HLKLNDQAQR TVLCKDPFRS VDERVERLIN SL
Expression Region 1-452
Protein Description full length protein
Storage Buffer Tris-based buffer,50% glycerol
Still Have Questions? Leave a Message or Start an on-line Chat

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