Recombinant Saccharomonospora viridis Pup--protein ligase(pafA)

Code CSB-YP512063SVE
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Source Yeast
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Code CSB-EP512063SVE
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Source E.coli
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Code CSB-EP512063SVE-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP512063SVE
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Source Baculovirus
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Code CSB-MP512063SVE
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names pafA
Uniprot No. C7MWV3
Alternative Names pafA; Svir_21990; Pup--protein ligase; EC 6.3.1.19; Proteasome accessory factor A; Pup-conjugating enzyme
Species Saccharomonospora viridis (strain ATCC 15386 / DSM 43017 / JCM 3036 / NBRC 12207 / P101)
Expression Region 1-452
Target Protein Sequence MQRRIFGIET EFGVTCTFHG QRRLSPDEVA RYLFRRVVSW GRSSNVFLSN GSRLYLDVGS HPEYATAECD DLAQLVTHDK AGERILEDLL IDAERRLAEE GIGGDIFLFK NNTDSAGNSY GCHENYLVTR AGEFSRVADV LLPFLVTRQL VCGAGKVLQT PRGGVYCLSQ RAEHIWEGVS SATTRSRPII NTRDEPHADA ERYRRLHVIV GDSNMAEPTT LLKVGSVHLV LQMIEEGVQF RDFTLDNPIR AIREISHDLT GRRQVRLAGG REASALEIQR EYYARAVQHV ESGDPSPTTQ YLIDLWGRAL DAVEQQDFSS IDTEIDWAIK HRLVERYRSK HNLTLSDPRV AQLDLAYHDI RRGRGVFDLL QRKGMVRRIT DDGEIELAKD SPPQTTRAKL RGDFIAAAQE AGRDFTVDWV HLKLNDQAQR TVLCKDPFRS VDERVERLIN SL
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Target Data

Function Catalyzes the covalent attachment of the prokaryotic ubiquitin-like protein modifier Pup to the proteasomal substrate proteins, thereby targeting them for proteasomal degradation. This tagging system is termed pupylation. The ligation reaction involves the side-chain carboxylate of the C-terminal glutamate of Pup and the side-chain amino group of a substrate lysine.
Protein Families Pup ligase/Pup deamidase family, Pup-conjugating enzyme subfamily
Database Links

KEGG: svi:Svir_21990

STRING: 471857.Svir_21990

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