Product Details

Purity

>85% (SDS-PAGE)

Target Names

DED1

Uniprot No.

P06634

Alternative Names

DED1; SPP81; YOR204WATP-dependent RNA helicase DED1; EC 3.6.4.13; DEAD box protein 1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

ATP-binding RNA helicase involved in translation initiation. Remodels RNA in response to ADP and ATP concentrations by facilitating disruption, but also formation of RNA duplexes. Has weak ATP-dependent affinity for dsRNA, but strong ATP-dependent affinity for ssRNA. Acts as a virus host factor involved in the replication of the MBV and the L-A viruses by promoting the negative-strand RNA synthesis. May be involved in recognition of the preinitiation complex and DNA binding of the RNA polymerase III and play a role in mRNA splicing.

Gene References into Functions

Ded1p affects translation initiation by controlling the use of near-cognate initiation codons that are proximal to mRNA structure in 5' untranslated regions PMID: 29950728
Oligomerization represents a previously unappreciated level of regulation for Ded1p DEAD-box helicase activities. PMID: 26212457
Individual members of the Ded1/DDX3 subfamily perform multiple functions in RNA metabolism in both nucleus and cytoplasm. [review] PMID: 25039764
The authors conclude that Ded1 is an mRNP cofactor of the cap complex that may function to remodel the different mRNPs and thereby regulate the expression of the mRNAs. PMID: 25013175
DEAD-box helicase Ded1p forms exceptionally long-lived complexes with RNA PMID: 24367975
Ded1p and glyceraldehyde-3-phosphate dehydrogenase enhance tombusvirus (+)-strand synthesis PMID: 22359508
Ded1 is a translation initiation factor that assembles and remodels an intermediate complex in translation initiation. PMID: 21925384
motif III and the binding energy of gamma-PO(4) of ATP are used to coordinate motifs I, II, and VI and the two RecA-like domains to create a high-affinity single-stranded RNA binding site PMID: 20026132
DED1 establishes an ATP-dependent steady state between unwinding & annealing, which enables the enzyme to modulate balance between the 2 opposing activities through ATP & ADP levels, by facilitating not only disruption but also formation of RNA duplexes. PMID: 16216083
In vitro measurements of DED1 disassembly of model ribonucleoproteins were made. PMID: 16556937
Ded1 uses the single-stranded region to gain access to the duplex, strand separation is directly initiated from the duplex region and no covalent connection between the single strand and the duplex region is required. PMID: 17072313
Ded1p is a bifunctional protein that can affect both translation initiation and P-body formation. PMID: 18162578
Intragenic suppressors of the F405L mutation in Ded1 mapped close to regions of the protein involved in ATP or RNA binding in DEAD-box crystal structures, which implicated a defect at this level. PMID: 18332124

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Subcellular Location

Cytoplasm.

Protein Families

DEAD box helicase family, DDX3/DED1 subfamily

Database Links

KEGG: sce:YOR204W

STRING: 4932.YOR204W

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Recombinant Saccharomyces cerevisiae ATP-dependent RNA helicase DED1 (DED1), partial

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