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Recombinant Saccharomyces cerevisiae ATP-dependent helicase NAM7 (NAM7), partial

Recombinant Saccharomyces cerevisiae ATP-dependent helicase NAM7 (NAM7), partial

Code	CSB-YP330024SVG
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP330024SVG
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP330024SVG-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP330024SVG
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP330024SVG
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

NAM7

Uniprot No.

P30771

Alternative Names

NAM7; IFS2; MOF4; UPF1; YMR080C; YM9582.05C; ATP-dependent helicase NAM7; EC 3.6.4.-; Nonsense-mediated mRNA decay protein 1; Nuclear accommodation of mitochondria 7 protein; Up-frameshift suppressor 1

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Probable helicase involved in mitochondrial functions. Required for rapid turnover of mRNAs containing a premature translational termination codon.

Gene References into Functions

The propose that the Sse1/Hsp70 complex maintains the solubility of FLAG-Pgk1-300, thereby stimulating its Upf-dependent degradation by the proteasomes. PMID: 28483531
The study provides evidence that two tyrosines within a phospho-motif of Upf1 act redundantly to promote ATP hydrolysis, Nonsense-Mediated mRNA Decay efficiency and translation termination fidelity. PMID: 24198248
The cysteine- and histidine-rich (CH) domain and the RNA helicase domain of yeast Upf1 can engage in two new types of molecular interactions: an intramolecular interaction between these two domains and self-association of each of these domains. PMID: 24100012
The upf1Delta mutant contained elevated copper levels in the vacuole relative to wild-type yeast cells, after both strains were exposed to elevated copper levels. Copper tolerance by the upf1Delta mutant is dependent on the presence of CTR2. PMID: 23450501
Results indicate that Upf1 plays a key role in a mechanism coupling termination and ribosome release at a premature termination codon to subsequent ribosome reutilization for another round of translation initiation. PMID: 20675403
Upf1 stimulates degradation of the product derived from aberrant messenger RNA containing a specific nonsense mutation by the proteasome PMID: 19798102
interaction between Upf1p and the nuclear pore proteins, Nup100p and Nup116p, is significant because it suggests a mechanism to ensure that Upf1p associates with newly synthesized mRNA as it is transported from the nucleus to the cytoplasm PMID: 15716093
Upf1p associates with direct substrates of the NMD pathway PMID: 18087042